Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch

This study reports enhanced degradation of crude oil hydrocarbons by a bacterial consortium culture (CC) immobilized onto sawdust (SD) and oil palms empty fruit bunch (OPEFB). The bacterial surface hydrophobic property of the bacterial CC was 60.3 ± 2.20 %, which suggested high bacterial cell attach...

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Main Authors: Hazaimeh, Mohammad, Abd Mutalib, Sahilah, Sannasi Abdullah, Palsan, Wong, Kok Kee, Surif, Salmijah
Format: Article
Language:English
Published: Springer Berlin Heidelberg 2014
Subjects:
Online Access:http://eprints.intimal.edu.my/41/
http://eprints.intimal.edu.my/41/1/Enhancedcrudeoilhydrocarbondegradationbyself-immobilized%20bacterial%20consortium%20culture%20on%20sawdust%20and%20oil%20palm%20empty%20fruit%20bunch%20%281%29.pdf
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author Hazaimeh, Mohammad
Abd Mutalib, Sahilah
Sannasi Abdullah, Palsan
Wong, Kok Kee
Surif, Salmijah
author_facet Hazaimeh, Mohammad
Abd Mutalib, Sahilah
Sannasi Abdullah, Palsan
Wong, Kok Kee
Surif, Salmijah
author_sort Hazaimeh, Mohammad
building INTI Institutional Repository
collection Online Access
description This study reports enhanced degradation of crude oil hydrocarbons by a bacterial consortium culture (CC) immobilized onto sawdust (SD) and oil palms empty fruit bunch (OPEFB). The bacterial surface hydrophobic property of the bacterial CC was 60.3 ± 2.20 %, which suggested high bacterial cell attachment onto the carrier materials. The free bacterial CC exhibited the ability to produce exopolysaccharide (EPS) in minimal salt medium (MSM). The highest value of EPS produced by free bacteria CC was 61.3 ± 2.10 %. Due to their surface hydrophobic properties and ability to produce EPS, the bacterial CC was self-immobilized onto OPEFB and SD. The immobilized bacterial CC was then used to degrade crude oil hydrocarbons. The results showed that bacterial CC immobilized onto OPEFB and SD increased degradation of crude oil by 17.52 % and, 15.85 % respectively, at week 6 of incubation, and shortened the time to complete degradation by 25 % (from 8 to 6 weeks) compared to free bacterial CC. Immobilized bacterial CC enhanced biosurfactant production, as indicated by the emulsification index (E24%). Thus, the present study demonstrated that bacterial CC immobilized onto carrier materials increases crude oil degradation by increasing production of biosurfactants.
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spelling intimal-412016-10-18T07:08:54Z http://eprints.intimal.edu.my/41/ Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch Hazaimeh, Mohammad Abd Mutalib, Sahilah Sannasi Abdullah, Palsan Wong, Kok Kee Surif, Salmijah QR Microbiology This study reports enhanced degradation of crude oil hydrocarbons by a bacterial consortium culture (CC) immobilized onto sawdust (SD) and oil palms empty fruit bunch (OPEFB). The bacterial surface hydrophobic property of the bacterial CC was 60.3 ± 2.20 %, which suggested high bacterial cell attachment onto the carrier materials. The free bacterial CC exhibited the ability to produce exopolysaccharide (EPS) in minimal salt medium (MSM). The highest value of EPS produced by free bacteria CC was 61.3 ± 2.10 %. Due to their surface hydrophobic properties and ability to produce EPS, the bacterial CC was self-immobilized onto OPEFB and SD. The immobilized bacterial CC was then used to degrade crude oil hydrocarbons. The results showed that bacterial CC immobilized onto OPEFB and SD increased degradation of crude oil by 17.52 % and, 15.85 % respectively, at week 6 of incubation, and shortened the time to complete degradation by 25 % (from 8 to 6 weeks) compared to free bacterial CC. Immobilized bacterial CC enhanced biosurfactant production, as indicated by the emulsification index (E24%). Thus, the present study demonstrated that bacterial CC immobilized onto carrier materials increases crude oil degradation by increasing production of biosurfactants. Springer Berlin Heidelberg 2014 Article PeerReviewed text en http://eprints.intimal.edu.my/41/1/Enhancedcrudeoilhydrocarbondegradationbyself-immobilized%20bacterial%20consortium%20culture%20on%20sawdust%20and%20oil%20palm%20empty%20fruit%20bunch%20%281%29.pdf Hazaimeh, Mohammad and Abd Mutalib, Sahilah and Sannasi Abdullah, Palsan and Wong, Kok Kee and Surif, Salmijah (2014) Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch. Annals of Microbiology, 64 (4). pp. 1769-1777. ISSN 1869-2044 10.1007/s13213-014-0821-3
spellingShingle QR Microbiology
Hazaimeh, Mohammad
Abd Mutalib, Sahilah
Sannasi Abdullah, Palsan
Wong, Kok Kee
Surif, Salmijah
Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch
title Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch
title_full Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch
title_fullStr Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch
title_full_unstemmed Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch
title_short Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch
title_sort enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch
topic QR Microbiology
url http://eprints.intimal.edu.my/41/
http://eprints.intimal.edu.my/41/
http://eprints.intimal.edu.my/41/1/Enhancedcrudeoilhydrocarbondegradationbyself-immobilized%20bacterial%20consortium%20culture%20on%20sawdust%20and%20oil%20palm%20empty%20fruit%20bunch%20%281%29.pdf