The Effects of pH and Cell Density to the Responses of Immobilized Cyanobacteria for Copper Detection
Rapid urbanization and industrialization lead to heavy metal pollution which poses a serious threat to human’s health. Thus, there is a continuing need to develop a device that can be easily used to monitor and detect the presence of heavy metal pollution. Whole cells e.g. cyanobacteria have be...
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| Format: | Article |
| Language: | English |
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Engineering and Technology Publishing
2014
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| Online Access: | http://eprints.intimal.edu.my/392/ http://eprints.intimal.edu.my/392/1/The%20Effects%20of%20pH%20and%20Cell%20Density%20to%20the....pdf |
| _version_ | 1848766465498415104 |
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| author | Kunasakaran, Rubban Vijayan, Sughan Wong, Ling Shing Chai, Mee Kin |
| author_facet | Kunasakaran, Rubban Vijayan, Sughan Wong, Ling Shing Chai, Mee Kin |
| author_sort | Kunasakaran, Rubban |
| building | INTI Institutional Repository |
| collection | Online Access |
| description | Rapid urbanization and industrialization lead to
heavy metal pollution which poses a serious threat to
human’s health. Thus, there is a continuing need to develop
a device that can be easily used to monitor and detect the
presence of heavy metal pollution. Whole cells e.g.
cyanobacteria have been reported as potential bio-indicators
for environmental heavy metals detection. However, the
intensity of the biological responses of these cells is affected
by pH and the cell density. In this paper, the effect of pH
and cell density to the response of cyanobacteria Anabaena
cylindrica to copper (Cu) is reported. The cyanobacteria
were cultured and immobilized with agarose in cuvettes
before the exposure to Cu solution (1.0 mg/L). The
measurement was carried out with OD= 680 nm using a
spectrophotometer, which indicated the changes of
chlorophyll. The most significant response of the cells was
measured at pH 8 and the optimal density of the cell for the
detection was 500,000 cell/mL. This study was an important
stage to determine the potential of A. cylindrica to be used in
heavy metal detection. |
| first_indexed | 2025-11-14T11:51:35Z |
| format | Article |
| id | intimal-392 |
| institution | INTI International University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:51:35Z |
| publishDate | 2014 |
| publisher | Engineering and Technology Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | intimal-3922016-09-23T02:34:08Z http://eprints.intimal.edu.my/392/ The Effects of pH and Cell Density to the Responses of Immobilized Cyanobacteria for Copper Detection Kunasakaran, Rubban Vijayan, Sughan Wong, Ling Shing Chai, Mee Kin TP Chemical technology Rapid urbanization and industrialization lead to heavy metal pollution which poses a serious threat to human’s health. Thus, there is a continuing need to develop a device that can be easily used to monitor and detect the presence of heavy metal pollution. Whole cells e.g. cyanobacteria have been reported as potential bio-indicators for environmental heavy metals detection. However, the intensity of the biological responses of these cells is affected by pH and the cell density. In this paper, the effect of pH and cell density to the response of cyanobacteria Anabaena cylindrica to copper (Cu) is reported. The cyanobacteria were cultured and immobilized with agarose in cuvettes before the exposure to Cu solution (1.0 mg/L). The measurement was carried out with OD= 680 nm using a spectrophotometer, which indicated the changes of chlorophyll. The most significant response of the cells was measured at pH 8 and the optimal density of the cell for the detection was 500,000 cell/mL. This study was an important stage to determine the potential of A. cylindrica to be used in heavy metal detection. Engineering and Technology Publishing 2014 Article PeerReviewed text en http://eprints.intimal.edu.my/392/1/The%20Effects%20of%20pH%20and%20Cell%20Density%20to%20the....pdf Kunasakaran, Rubban and Vijayan, Sughan and Wong, Ling Shing and Chai, Mee Kin (2014) The Effects of pH and Cell Density to the Responses of Immobilized Cyanobacteria for Copper Detection. Journal of Life Sciences and Technologies, 2 (2). ISSN 2301-3672 http://www.jolst.net/ 10.12720/jolst.2.2.78-81 |
| spellingShingle | TP Chemical technology Kunasakaran, Rubban Vijayan, Sughan Wong, Ling Shing Chai, Mee Kin The Effects of pH and Cell Density to the Responses of Immobilized Cyanobacteria for Copper Detection |
| title | The Effects of pH and Cell Density to the
Responses of Immobilized Cyanobacteria for
Copper Detection |
| title_full | The Effects of pH and Cell Density to the
Responses of Immobilized Cyanobacteria for
Copper Detection |
| title_fullStr | The Effects of pH and Cell Density to the
Responses of Immobilized Cyanobacteria for
Copper Detection |
| title_full_unstemmed | The Effects of pH and Cell Density to the
Responses of Immobilized Cyanobacteria for
Copper Detection |
| title_short | The Effects of pH and Cell Density to the
Responses of Immobilized Cyanobacteria for
Copper Detection |
| title_sort | effects of ph and cell density to the
responses of immobilized cyanobacteria for
copper detection |
| topic | TP Chemical technology |
| url | http://eprints.intimal.edu.my/392/ http://eprints.intimal.edu.my/392/ http://eprints.intimal.edu.my/392/ http://eprints.intimal.edu.my/392/1/The%20Effects%20of%20pH%20and%20Cell%20Density%20to%20the....pdf |