The Effects of pH and Cell Density to the Responses of Immobilized Cyanobacteria for Copper Detection
Rapid urbanization and industrialization lead to heavy metal pollution which poses a serious threat to human’s health. Thus, there is a continuing need to develop a device that can be easily used to monitor and detect the presence of heavy metal pollution. Whole cells e.g. cyanobacteria have be...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Engineering and Technology Publishing
2014
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| Subjects: | |
| Online Access: | http://eprints.intimal.edu.my/392/ http://eprints.intimal.edu.my/392/1/The%20Effects%20of%20pH%20and%20Cell%20Density%20to%20the....pdf |
| Summary: | Rapid urbanization and industrialization lead to
heavy metal pollution which poses a serious threat to
human’s health. Thus, there is a continuing need to develop
a device that can be easily used to monitor and detect the
presence of heavy metal pollution. Whole cells e.g.
cyanobacteria have been reported as potential bio-indicators
for environmental heavy metals detection. However, the
intensity of the biological responses of these cells is affected
by pH and the cell density. In this paper, the effect of pH
and cell density to the response of cyanobacteria Anabaena
cylindrica to copper (Cu) is reported. The cyanobacteria
were cultured and immobilized with agarose in cuvettes
before the exposure to Cu solution (1.0 mg/L). The
measurement was carried out with OD= 680 nm using a
spectrophotometer, which indicated the changes of
chlorophyll. The most significant response of the cells was
measured at pH 8 and the optimal density of the cell for the
detection was 500,000 cell/mL. This study was an important
stage to determine the potential of A. cylindrica to be used in
heavy metal detection. |
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