Rapid Detection of Heavy Metals with the Response of Carotenoids in Daucus Carota
In this paper, a rapid detection method for heavy metals (Cu, Pb and Zn) is reported. The method was based on the response of natural cell-bounded carotenoids in Daucus carota to short term exposure of heavy metals. The cells were cultured for 90 days in dark. The presence of carotenoids in cel...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
IJESD
2014
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| Subjects: | |
| Online Access: | http://eprints.intimal.edu.my/388/ http://eprints.intimal.edu.my/388/1/Rapid%20Detection%20of%20Heavy%20Metals%20with%20the%20Response%20of...pdf |
| Summary: | In this paper, a rapid detection method for heavy
metals (Cu, Pb and Zn) is reported. The method was based on
the response of natural cell-bounded carotenoids in Daucus
carota to short term exposure of heavy metals. The cells were
cultured for 90 days in dark. The presence of carotenoids in
cells was confirmed spectrometrically with optical density (OD)
measured at λ= 450 nm. The responses of the cells to 0.01, 0.10,
and 1.00 ppm of heavy metals were confirmed by the increase of
OD after the short term exposure, with the lowest detection
were recorded at 0.01, 0.10, and 0.01 ppm respectively. The OD
increase might be a result of the synthesis of carotenoids, which
was probably triggered by the increase of oxidative stress. With
an average optimum response time of 40 minutes, the cell could
be potentially utilized in biosensor. |
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