Tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) by a metal acclimatized bacterial consortium culture

This investigation reports the tolerance and biodegradation of benzene, toluene, ethylbenzene and xylene isomers (BTEX) by a heavy metal-adapted environmental bacterial consortium, known as consortium culture (CC). Higher tolerance was observed with benzene (IC50 value up to 191.25 mg/L), foll...

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Main Authors: Fellie, E. A., Sannasi, P., Wong, K. K, Salmijah, S., Kader, J.
Format: Article
Language:English
Published: 2012
Subjects:
Online Access:http://eprints.intimal.edu.my/141/
http://eprints.intimal.edu.my/141/1/26_ft.pdf
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author Fellie, E. A.
Sannasi, P.
Wong, K. K
Salmijah, S.
Kader, J.
author_facet Fellie, E. A.
Sannasi, P.
Wong, K. K
Salmijah, S.
Kader, J.
author_sort Fellie, E. A.
building INTI Institutional Repository
collection Online Access
description This investigation reports the tolerance and biodegradation of benzene, toluene, ethylbenzene and xylene isomers (BTEX) by a heavy metal-adapted environmental bacterial consortium, known as consortium culture (CC). Higher tolerance was observed with benzene (IC50 value up to 191.25 mg/L), followed by toluene (IC50 = 139.67 mg/L), xylene (IC50 = 97.04 mg/L) and ethylbenzene (IC50 =96.99 mg/L). Significant decrease (p < 0.05) in the specific growth rate (µ), however was observed as the concentrations of each individual BTEX were increased from 10 mg/L to 500 mg/L. Growth of CC was completely inhibited at 250 mg/L ethylbenzene and 500 mg/L xylene. Toxicity followed the trend: B<T<X<E. Biodegradation of individual BTEX compound was monitored by gas chromatography. The GC-FID chromatographic profiles showed the capability of CC to significantly biodegrade (p < 0.05) benzene (61.66 %), toluene (55.91 %), ethylbenzene (37.15 %), pxylene (43.66 %), m-xylene (47.86 %) and o-xylene (41.03 %) at an initial concentration of 50 mg/L after 48 hours. These findings confirm the ability of CC to withstand biodegrade and utilize BTEX as the sole source of carbon and energy in the following order: B>T>X>E.
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spelling intimal-1412016-04-06T10:11:03Z http://eprints.intimal.edu.my/141/ Tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) by a metal acclimatized bacterial consortium culture Fellie, E. A. Sannasi, P. Wong, K. K Salmijah, S. Kader, J. TP Chemical technology This investigation reports the tolerance and biodegradation of benzene, toluene, ethylbenzene and xylene isomers (BTEX) by a heavy metal-adapted environmental bacterial consortium, known as consortium culture (CC). Higher tolerance was observed with benzene (IC50 value up to 191.25 mg/L), followed by toluene (IC50 = 139.67 mg/L), xylene (IC50 = 97.04 mg/L) and ethylbenzene (IC50 =96.99 mg/L). Significant decrease (p < 0.05) in the specific growth rate (µ), however was observed as the concentrations of each individual BTEX were increased from 10 mg/L to 500 mg/L. Growth of CC was completely inhibited at 250 mg/L ethylbenzene and 500 mg/L xylene. Toxicity followed the trend: B<T<X<E. Biodegradation of individual BTEX compound was monitored by gas chromatography. The GC-FID chromatographic profiles showed the capability of CC to significantly biodegrade (p < 0.05) benzene (61.66 %), toluene (55.91 %), ethylbenzene (37.15 %), pxylene (43.66 %), m-xylene (47.86 %) and o-xylene (41.03 %) at an initial concentration of 50 mg/L after 48 hours. These findings confirm the ability of CC to withstand biodegrade and utilize BTEX as the sole source of carbon and energy in the following order: B>T>X>E. 2012 Article PeerReviewed text en http://eprints.intimal.edu.my/141/1/26_ft.pdf Fellie, E. A. and Sannasi, P. and Wong, K. K and Salmijah, S. and Kader, J. (2012) Tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) by a metal acclimatized bacterial consortium culture. Research Journal of Biotechnology, 7 (3). pp. 52-58. ISSN 2278-4535
spellingShingle TP Chemical technology
Fellie, E. A.
Sannasi, P.
Wong, K. K
Salmijah, S.
Kader, J.
Tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) by a metal acclimatized bacterial consortium culture
title Tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) by a metal acclimatized bacterial consortium culture
title_full Tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) by a metal acclimatized bacterial consortium culture
title_fullStr Tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) by a metal acclimatized bacterial consortium culture
title_full_unstemmed Tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) by a metal acclimatized bacterial consortium culture
title_short Tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (BTEX) by a metal acclimatized bacterial consortium culture
title_sort tolerance and biodegradation of benzene, toluene, ethylbenzene and xylenes (btex) by a metal acclimatized bacterial consortium culture
topic TP Chemical technology
url http://eprints.intimal.edu.my/141/
http://eprints.intimal.edu.my/141/1/26_ft.pdf