Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes

Zinc oxide nanoparticles (ZnO NPs) have been used in the treatment of bacterial infections due to their antibacterial properties. The present study was aimed to explore the antibacterial effects of ZnO NPs on Streptococcus pyogenes. The antibacterial properties were evaluated by investigating the gr...

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Main Authors: Liang, Sharolynne, Xiao Tong, Wong*, Ling Shing, Lim, Yang Mooi, Lee, Poh Foong, Sinouvassane, Djearamane
Format: Article
Published: Elsevier 2020
Subjects:
Online Access:http://eprints.intimal.edu.my/1401/
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author Liang, Sharolynne, Xiao Tong
Wong*, Ling Shing
Lim, Yang Mooi
Lee, Poh Foong
Sinouvassane, Djearamane
author_facet Liang, Sharolynne, Xiao Tong
Wong*, Ling Shing
Lim, Yang Mooi
Lee, Poh Foong
Sinouvassane, Djearamane
author_sort Liang, Sharolynne, Xiao Tong
building INTI Institutional Repository
collection Online Access
description Zinc oxide nanoparticles (ZnO NPs) have been used in the treatment of bacterial infections due to their antibacterial properties. The present study was aimed to explore the antibacterial effects of ZnO NPs on Streptococcus pyogenes. The antibacterial properties were evaluated by investigating the growth inhibition and oxidative stress effects of ZnO NPs on S. pyogenes. The bacterial growth inhibition was determined through the percentage of reduction in the turbidity of bacterial suspension and bacterial colony upon treating with ZnO NPs, while the oxidative stress of ZnO NPs on bacterial cells were assessed by measuring the levels of reactive oxygen species (ROS) and lipid peroxidation. The results showed the dose dependent and significant (p < 0.05) growth inhibition and oxidative stress effects on S. pyogenes for 10 to 100 μg/mL ZnO NPs at 24 h. Fourier-transform infrared spectrometry (FTIR) analysis confirmed the binding of ZnO NPs to polypeptides and glycogen from the bacterial cell wall. Further, the scanning electron microscopy (SEM) results showed the attachment of ZnO NPs to bacterial cell wall and the resultant cell wall disruption and cell damage. The study results confirmed the antimicrobial effects of ZnO NPs on S. pyogenes.
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publishDate 2020
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spelling intimal-14012020-09-01T03:41:05Z http://eprints.intimal.edu.my/1401/ Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes Liang, Sharolynne, Xiao Tong Wong*, Ling Shing Lim, Yang Mooi Lee, Poh Foong Sinouvassane, Djearamane Q Science (General) QD Chemistry TP Chemical technology Zinc oxide nanoparticles (ZnO NPs) have been used in the treatment of bacterial infections due to their antibacterial properties. The present study was aimed to explore the antibacterial effects of ZnO NPs on Streptococcus pyogenes. The antibacterial properties were evaluated by investigating the growth inhibition and oxidative stress effects of ZnO NPs on S. pyogenes. The bacterial growth inhibition was determined through the percentage of reduction in the turbidity of bacterial suspension and bacterial colony upon treating with ZnO NPs, while the oxidative stress of ZnO NPs on bacterial cells were assessed by measuring the levels of reactive oxygen species (ROS) and lipid peroxidation. The results showed the dose dependent and significant (p < 0.05) growth inhibition and oxidative stress effects on S. pyogenes for 10 to 100 μg/mL ZnO NPs at 24 h. Fourier-transform infrared spectrometry (FTIR) analysis confirmed the binding of ZnO NPs to polypeptides and glycogen from the bacterial cell wall. Further, the scanning electron microscopy (SEM) results showed the attachment of ZnO NPs to bacterial cell wall and the resultant cell wall disruption and cell damage. The study results confirmed the antimicrobial effects of ZnO NPs on S. pyogenes. Elsevier 2020-10-01 Article PeerReviewed Liang, Sharolynne, Xiao Tong and Wong*, Ling Shing and Lim, Yang Mooi and Lee, Poh Foong and Sinouvassane, Djearamane (2020) Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes. South African Journal of Chemical Engineering, 34. pp. 63-71. ISSN 1026-9185 https://doi.org/10.1016/j.sajce.2020.05.009
spellingShingle Q Science (General)
QD Chemistry
TP Chemical technology
Liang, Sharolynne, Xiao Tong
Wong*, Ling Shing
Lim, Yang Mooi
Lee, Poh Foong
Sinouvassane, Djearamane
Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes
title Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes
title_full Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes
title_fullStr Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes
title_full_unstemmed Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes
title_short Effects of Zinc Oxide nanoparticles on Streptococcus pyogenes
title_sort effects of zinc oxide nanoparticles on streptococcus pyogenes
topic Q Science (General)
QD Chemistry
TP Chemical technology
url http://eprints.intimal.edu.my/1401/
http://eprints.intimal.edu.my/1401/