ANTIBIOGRAM PATTERNS OF STAPHYLOCOCCAL SPECIES ISOLATED FROM HEALTHY INDIVIDUALS IN NILAI, NEGERI SEMBILAN, MALAYSIA

Antibiotic resistance is an alarming phenomenon worldwide, challenging the effectiveness of antibiotics which are used to treat infectious diseases. In this study, pure cultures of forty skin and nasal samples from healthy individuals in Nilai, Negeri Sembilan, were screened. Samples were inoculat...

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Main Authors:	Himashi, Imanda Gurudeniya, Subramaniam, Geetha
Format:	Article
Language:	English
Published:	Global Science Publications 2018
Subjects:	R Medicine (General)
Online Access:	http://eprints.intimal.edu.my/1250/ http://eprints.intimal.edu.my/1250/1/AJ-45%20Dr%20Geetha%20Dec%202018.pdf

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author	Himashi, Imanda Gurudeniya Subramaniam, Geetha
author_facet	Himashi, Imanda Gurudeniya Subramaniam, Geetha
author_sort	Himashi, Imanda Gurudeniya
building	INTI Institutional Repository
collection	Online Access
description	Antibiotic resistance is an alarming phenomenon worldwide, challenging the effectiveness of antibiotics which are used to treat infectious diseases. In this study, pure cultures of forty skin and nasal samples from healthy individuals in Nilai, Negeri Sembilan, were screened. Samples were inoculated on the nutrient agar plates to obtain single colonies, followed by sub-culturing and series of confirmatory tests such as Gram staining, catalase test, Mannitol Salt Agar, as well as cefoxitin disk-diffusion test to obtain pure MRSA and MRSE isolates. A total of 13 MRSA strains and one MRSE strain were obtained and subjected to antibiotic susceptibility testing on Mueller Hinton agar using Kirby-Bauer disk diffusion technique. All cefoxitin-resistant isolateswere resistant to penicillin G, erythromycin, linezolid, and vancomycin; 78% of them were resistant to teicoplanin, clindamycin, quinupristin/dalfopristin; 56% showed resistance to nalidixic acid and cefazolin while 33% of them showed resistance to cefuroxime. All isolates (100%) were susceptible to doxycycline, chloramphenicol, and ciprofloxacin. Eight different antibiogram patterns were obtained from these 14 isolates with resistance towards 11 out of 16 types of antibiotics used in this study. This finding which is a surveillance control method of antibiotic-resistant crisis is important as the results could provide an indication of the resistance patterns circulating among healthy individuals, and could contribute towards a more definitive antibiotic therapy of staphylococcal species infections in Malaysia
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format	Article
id	intimal-1250
institution	INTI International University
institution_category	Local University
language	English
last_indexed	2025-11-14T11:55:04Z
publishDate	2018
publisher	Global Science Publications
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spelling	intimal-12502018-11-23T06:48:34Z http://eprints.intimal.edu.my/1250/ ANTIBIOGRAM PATTERNS OF STAPHYLOCOCCAL SPECIES ISOLATED FROM HEALTHY INDIVIDUALS IN NILAI, NEGERI SEMBILAN, MALAYSIA Himashi, Imanda Gurudeniya Subramaniam, Geetha R Medicine (General) Antibiotic resistance is an alarming phenomenon worldwide, challenging the effectiveness of antibiotics which are used to treat infectious diseases. In this study, pure cultures of forty skin and nasal samples from healthy individuals in Nilai, Negeri Sembilan, were screened. Samples were inoculated on the nutrient agar plates to obtain single colonies, followed by sub-culturing and series of confirmatory tests such as Gram staining, catalase test, Mannitol Salt Agar, as well as cefoxitin disk-diffusion test to obtain pure MRSA and MRSE isolates. A total of 13 MRSA strains and one MRSE strain were obtained and subjected to antibiotic susceptibility testing on Mueller Hinton agar using Kirby-Bauer disk diffusion technique. All cefoxitin-resistant isolateswere resistant to penicillin G, erythromycin, linezolid, and vancomycin; 78% of them were resistant to teicoplanin, clindamycin, quinupristin/dalfopristin; 56% showed resistance to nalidixic acid and cefazolin while 33% of them showed resistance to cefuroxime. All isolates (100%) were susceptible to doxycycline, chloramphenicol, and ciprofloxacin. Eight different antibiogram patterns were obtained from these 14 isolates with resistance towards 11 out of 16 types of antibiotics used in this study. This finding which is a surveillance control method of antibiotic-resistant crisis is important as the results could provide an indication of the resistance patterns circulating among healthy individuals, and could contribute towards a more definitive antibiotic therapy of staphylococcal species infections in Malaysia Global Science Publications 2018-12-01 Article PeerReviewed text en http://eprints.intimal.edu.my/1250/1/AJ-45%20Dr%20Geetha%20Dec%202018.pdf Himashi, Imanda Gurudeniya and Subramaniam, Geetha (2018) ANTIBIOGRAM PATTERNS OF STAPHYLOCOCCAL SPECIES ISOLATED FROM HEALTHY INDIVIDUALS IN NILAI, NEGERI SEMBILAN, MALAYSIA. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 20 (4). pp. 1343-1348. ISSN 0972-3005
spellingShingle	R Medicine (General) Himashi, Imanda Gurudeniya Subramaniam, Geetha ANTIBIOGRAM PATTERNS OF STAPHYLOCOCCAL SPECIES ISOLATED FROM HEALTHY INDIVIDUALS IN NILAI, NEGERI SEMBILAN, MALAYSIA
title	ANTIBIOGRAM PATTERNS OF STAPHYLOCOCCAL SPECIES ISOLATED FROM HEALTHY INDIVIDUALS IN NILAI, NEGERI SEMBILAN, MALAYSIA
title_full	ANTIBIOGRAM PATTERNS OF STAPHYLOCOCCAL SPECIES ISOLATED FROM HEALTHY INDIVIDUALS IN NILAI, NEGERI SEMBILAN, MALAYSIA
title_fullStr	ANTIBIOGRAM PATTERNS OF STAPHYLOCOCCAL SPECIES ISOLATED FROM HEALTHY INDIVIDUALS IN NILAI, NEGERI SEMBILAN, MALAYSIA
title_full_unstemmed	ANTIBIOGRAM PATTERNS OF STAPHYLOCOCCAL SPECIES ISOLATED FROM HEALTHY INDIVIDUALS IN NILAI, NEGERI SEMBILAN, MALAYSIA
title_short	ANTIBIOGRAM PATTERNS OF STAPHYLOCOCCAL SPECIES ISOLATED FROM HEALTHY INDIVIDUALS IN NILAI, NEGERI SEMBILAN, MALAYSIA
title_sort	antibiogram patterns of staphylococcal species isolated from healthy individuals in nilai, negeri sembilan, malaysia
topic	R Medicine (General)
url	http://eprints.intimal.edu.my/1250/ http://eprints.intimal.edu.my/1250/1/AJ-45%20Dr%20Geetha%20Dec%202018.pdf

ANTIBIOGRAM PATTERNS OF STAPHYLOCOCCAL SPECIES ISOLATED FROM HEALTHY INDIVIDUALS IN NILAI, NEGERI SEMBILAN, MALAYSIA

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