QUANTIFICATION OF ASTAXANTHIN PRODUCTION BY Haematococcus pluvialis INDUCED BY COPPPER NANOPARTICLES

Haematococcus pluvialis is green microalgae that is found in harsh-free regions like Africa, Europe and North America. It has the ability to produce high yield of astaxanthin under stress as such as when exposed to high temperature, pH, salinity, light and nitrogen deficiency. Astaxanthin is an unsa...

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Main Author: Thinakren, Batumalai
Format: Thesis
Language:English
Published: 2018
Subjects:
Online Access:http://eprints.intimal.edu.my/1132/
http://eprints.intimal.edu.my/1132/1/BBTEI%20178.pdf
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author Thinakren, Batumalai
author_facet Thinakren, Batumalai
author_sort Thinakren, Batumalai
building INTI Institutional Repository
collection Online Access
description Haematococcus pluvialis is green microalgae that is found in harsh-free regions like Africa, Europe and North America. It has the ability to produce high yield of astaxanthin under stress as such as when exposed to high temperature, pH, salinity, light and nitrogen deficiency. Astaxanthin is an unsaturated antioxidant that can also be found in various marine animals and microorganism. Astaxanthin were produced under stress condition. The aim of this study is to (i) develop H. pluvialis culture and (ii) investigate the effect of Cu nanoparticles to the production of astaxanthin in H. pluvialis. The H. pluvialis cells were grown in laboratory condition using circadian light, plus their growth patterns and cell count were measured using cell count chamber and light microscope and further analysed under 470 nm in the spectrophotometer in every 2 -3 days. The H. pluvialis cells were induced with different concentration of Cu nanoparticle at 100 mg/L, 200 mg/L and 300 mg/L. The astaxanthin were extracted and quantified at 470 nm using a spectrophotometer in every 4 - 5 days. The data were statistically analyzed using simple tools in Microsoft Excel 2016. Copper nanoparticles cannot enhance the production of astaxanthin in H. pluvialis due to high toxic level.
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spelling intimal-11322018-10-03T02:26:56Z http://eprints.intimal.edu.my/1132/ QUANTIFICATION OF ASTAXANTHIN PRODUCTION BY Haematococcus pluvialis INDUCED BY COPPPER NANOPARTICLES Thinakren, Batumalai TP Chemical technology Haematococcus pluvialis is green microalgae that is found in harsh-free regions like Africa, Europe and North America. It has the ability to produce high yield of astaxanthin under stress as such as when exposed to high temperature, pH, salinity, light and nitrogen deficiency. Astaxanthin is an unsaturated antioxidant that can also be found in various marine animals and microorganism. Astaxanthin were produced under stress condition. The aim of this study is to (i) develop H. pluvialis culture and (ii) investigate the effect of Cu nanoparticles to the production of astaxanthin in H. pluvialis. The H. pluvialis cells were grown in laboratory condition using circadian light, plus their growth patterns and cell count were measured using cell count chamber and light microscope and further analysed under 470 nm in the spectrophotometer in every 2 -3 days. The H. pluvialis cells were induced with different concentration of Cu nanoparticle at 100 mg/L, 200 mg/L and 300 mg/L. The astaxanthin were extracted and quantified at 470 nm using a spectrophotometer in every 4 - 5 days. The data were statistically analyzed using simple tools in Microsoft Excel 2016. Copper nanoparticles cannot enhance the production of astaxanthin in H. pluvialis due to high toxic level. 2018-01-01 Thesis NonPeerReviewed text en http://eprints.intimal.edu.my/1132/1/BBTEI%20178.pdf Thinakren, Batumalai (2018) QUANTIFICATION OF ASTAXANTHIN PRODUCTION BY Haematococcus pluvialis INDUCED BY COPPPER NANOPARTICLES. Other thesis, INTI INTERNATIONAL UNIVERSITY.
spellingShingle TP Chemical technology
Thinakren, Batumalai
QUANTIFICATION OF ASTAXANTHIN PRODUCTION BY Haematococcus pluvialis INDUCED BY COPPPER NANOPARTICLES
title QUANTIFICATION OF ASTAXANTHIN PRODUCTION BY Haematococcus pluvialis INDUCED BY COPPPER NANOPARTICLES
title_full QUANTIFICATION OF ASTAXANTHIN PRODUCTION BY Haematococcus pluvialis INDUCED BY COPPPER NANOPARTICLES
title_fullStr QUANTIFICATION OF ASTAXANTHIN PRODUCTION BY Haematococcus pluvialis INDUCED BY COPPPER NANOPARTICLES
title_full_unstemmed QUANTIFICATION OF ASTAXANTHIN PRODUCTION BY Haematococcus pluvialis INDUCED BY COPPPER NANOPARTICLES
title_short QUANTIFICATION OF ASTAXANTHIN PRODUCTION BY Haematococcus pluvialis INDUCED BY COPPPER NANOPARTICLES
title_sort quantification of astaxanthin production by haematococcus pluvialis induced by coppper nanoparticles
topic TP Chemical technology
url http://eprints.intimal.edu.my/1132/
http://eprints.intimal.edu.my/1132/1/BBTEI%20178.pdf