A New Method for Heavy Metals and Aluminium Detection using Biopolymer-based Optical Biosensor

A biopolymer-based biosensor for heavy metals and aluminium (Al) detection was constructed with naturally occurring β-carotene in palm kernel oil used as the biological reporter. The biosensor was designed with β-carotene entrapped by polyurethane- a polymer formed through prepolymerization of...

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Main Authors: Ling, Shing Wong, Chee, Sien Wong
Format: Article
Language:English
Published: IEEE 2015
Subjects:
Online Access:http://eprints.intimal.edu.my/11/
http://eprints.intimal.edu.my/11/1/A%20New%20Method%20for%20Heavy%20Metals%20and%20Aluminium%20Detection%20using%20Biopolymer-based%20Optical%20Biosensor.pdf
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author Ling, Shing Wong
Chee, Sien Wong
author_facet Ling, Shing Wong
Chee, Sien Wong
author_sort Ling, Shing Wong
building INTI Institutional Repository
collection Online Access
description A biopolymer-based biosensor for heavy metals and aluminium (Al) detection was constructed with naturally occurring β-carotene in palm kernel oil used as the biological reporter. The biosensor was designed with β-carotene entrapped by polyurethane- a polymer formed through prepolymerization of palm kernel oil. The presence of copper (Cu), lead (Pb), zinc (Zn), and Al was detected through the emulsification of β- carotene, which caused the change of optical density (OD) at λ = 450 nm. The results showed the OD increased with the presence of heavy metals and Al within 0.1 mg/L – 10.0 mg/L. The biosensor was constructed without extra steps to immobilize the biological component and it was simple to use with one step detection. Together with high reproducibility and fast response to Cu within 15 minutes, the biosensor showed good potential to be developed as a method to detect the presence of heavy metals and Al.
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spelling intimal-112016-09-14T03:39:27Z http://eprints.intimal.edu.my/11/ A New Method for Heavy Metals and Aluminium Detection using Biopolymer-based Optical Biosensor Ling, Shing Wong Chee, Sien Wong TP Chemical technology A biopolymer-based biosensor for heavy metals and aluminium (Al) detection was constructed with naturally occurring β-carotene in palm kernel oil used as the biological reporter. The biosensor was designed with β-carotene entrapped by polyurethane- a polymer formed through prepolymerization of palm kernel oil. The presence of copper (Cu), lead (Pb), zinc (Zn), and Al was detected through the emulsification of β- carotene, which caused the change of optical density (OD) at λ = 450 nm. The results showed the OD increased with the presence of heavy metals and Al within 0.1 mg/L – 10.0 mg/L. The biosensor was constructed without extra steps to immobilize the biological component and it was simple to use with one step detection. Together with high reproducibility and fast response to Cu within 15 minutes, the biosensor showed good potential to be developed as a method to detect the presence of heavy metals and Al. IEEE 2015-01 Article PeerReviewed text en http://eprints.intimal.edu.my/11/1/A%20New%20Method%20for%20Heavy%20Metals%20and%20Aluminium%20Detection%20using%20Biopolymer-based%20Optical%20Biosensor.pdf Ling, Shing Wong and Chee, Sien Wong (2015) A New Method for Heavy Metals and Aluminium Detection using Biopolymer-based Optical Biosensor. IEEE Sensors Journal, 15 (1). pp. 471-475. ISSN 1530-437X 10.1109/JSEN.2014.2345583
spellingShingle TP Chemical technology
Ling, Shing Wong
Chee, Sien Wong
A New Method for Heavy Metals and Aluminium Detection using Biopolymer-based Optical Biosensor
title A New Method for Heavy Metals and Aluminium Detection using Biopolymer-based Optical Biosensor
title_full A New Method for Heavy Metals and Aluminium Detection using Biopolymer-based Optical Biosensor
title_fullStr A New Method for Heavy Metals and Aluminium Detection using Biopolymer-based Optical Biosensor
title_full_unstemmed A New Method for Heavy Metals and Aluminium Detection using Biopolymer-based Optical Biosensor
title_short A New Method for Heavy Metals and Aluminium Detection using Biopolymer-based Optical Biosensor
title_sort new method for heavy metals and aluminium detection using biopolymer-based optical biosensor
topic TP Chemical technology
url http://eprints.intimal.edu.my/11/
http://eprints.intimal.edu.my/11/
http://eprints.intimal.edu.my/11/1/A%20New%20Method%20for%20Heavy%20Metals%20and%20Aluminium%20Detection%20using%20Biopolymer-based%20Optical%20Biosensor.pdf