Lipid peroxidation in chronic cerebral hypoperfusion- induced neurodegeneration in rats

Introduction: Reduced cerebral blood fl ow is associated with neurodegenerative disorders and dementia, in particular. Experimental evidence has demonstrated the initiating role of chronic cerebral hypoperfusion in neuronal damage to the hippocampus, the cerebral cortex, the white matter areas and...

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Main Authors: Saxena, Anil Kumar, Abdul Majeed, Saif Saad, Oothuman, Pakeer, Mustafa Mahmoud, Mohammed Imad Al-Deen
Format: Article
Language:English
Published: Faculty of Medicine, International Islamic University Malaysia 2011
Subjects:
Online Access:http://irep.iium.edu.my/9981/
http://irep.iium.edu.my/9981/1/download.pdf
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author Saxena, Anil Kumar
Abdul Majeed, Saif Saad
Oothuman, Pakeer
Mustafa Mahmoud, Mohammed Imad Al-Deen
author_facet Saxena, Anil Kumar
Abdul Majeed, Saif Saad
Oothuman, Pakeer
Mustafa Mahmoud, Mohammed Imad Al-Deen
author_sort Saxena, Anil Kumar
building IIUM Repository
collection Online Access
description Introduction: Reduced cerebral blood fl ow is associated with neurodegenerative disorders and dementia, in particular. Experimental evidence has demonstrated the initiating role of chronic cerebral hypoperfusion in neuronal damage to the hippocampus, the cerebral cortex, the white matter areas and the visual system. Permanent, bilateral occlusion of the common carotid arteries of rats (two vessel occlusion - 2VO) has been introduced for the reproduction of chronic cerebral hypoperfusion as it occurs in Alzheimer’s disease and human aging. Increased generation of free radicals through lipid peroxidation can damage neuronal cell membrane. Markers of lipid peroxidation have been found to be elevated in brain tissues and body fluids in neurodegenerative diseases, including Alzheimer’s disease, Parkinson disease and amyotrophic lateral sclerosis. Materials and Methods: Malondialdehyde (MDA), final product of lipid peroxidation, was estimated by thiobarbituric acid-reactive substances (TBARS) assay kit at eight weeks after induction of 2VO in the rats and control group. Results: Our study revealed a highly signifi cant (p<0.001) increase in the mean MDA concentration (12.296 ± 1.113 ìM) in 2VO rats as compared to the control group (5.286 ± 0.363 ìM) rats. Conclusion: Therapeutic strategies to modulate lipid peroxidation early throughout the course of the disease may be promising in slowing or possibly preventing neurodegenerative disorders.
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spelling iium-99812013-04-11T03:17:38Z http://irep.iium.edu.my/9981/ Lipid peroxidation in chronic cerebral hypoperfusion- induced neurodegeneration in rats Saxena, Anil Kumar Abdul Majeed, Saif Saad Oothuman, Pakeer Mustafa Mahmoud, Mohammed Imad Al-Deen RC321 Neuroscience. Biological psychiatry. Neuropsychiatry Introduction: Reduced cerebral blood fl ow is associated with neurodegenerative disorders and dementia, in particular. Experimental evidence has demonstrated the initiating role of chronic cerebral hypoperfusion in neuronal damage to the hippocampus, the cerebral cortex, the white matter areas and the visual system. Permanent, bilateral occlusion of the common carotid arteries of rats (two vessel occlusion - 2VO) has been introduced for the reproduction of chronic cerebral hypoperfusion as it occurs in Alzheimer’s disease and human aging. Increased generation of free radicals through lipid peroxidation can damage neuronal cell membrane. Markers of lipid peroxidation have been found to be elevated in brain tissues and body fluids in neurodegenerative diseases, including Alzheimer’s disease, Parkinson disease and amyotrophic lateral sclerosis. Materials and Methods: Malondialdehyde (MDA), final product of lipid peroxidation, was estimated by thiobarbituric acid-reactive substances (TBARS) assay kit at eight weeks after induction of 2VO in the rats and control group. Results: Our study revealed a highly signifi cant (p<0.001) increase in the mean MDA concentration (12.296 ± 1.113 ìM) in 2VO rats as compared to the control group (5.286 ± 0.363 ìM) rats. Conclusion: Therapeutic strategies to modulate lipid peroxidation early throughout the course of the disease may be promising in slowing or possibly preventing neurodegenerative disorders. Faculty of Medicine, International Islamic University Malaysia 2011-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/9981/1/download.pdf Saxena, Anil Kumar and Abdul Majeed, Saif Saad and Oothuman, Pakeer and Mustafa Mahmoud, Mohammed Imad Al-Deen (2011) Lipid peroxidation in chronic cerebral hypoperfusion- induced neurodegeneration in rats. International Medical Journal Malaysia, 10 (2). pp. 3-6. ISSN 1823-4631 http://www.e-imj.com/
spellingShingle RC321 Neuroscience. Biological psychiatry. Neuropsychiatry
Saxena, Anil Kumar
Abdul Majeed, Saif Saad
Oothuman, Pakeer
Mustafa Mahmoud, Mohammed Imad Al-Deen
Lipid peroxidation in chronic cerebral hypoperfusion- induced neurodegeneration in rats
title Lipid peroxidation in chronic cerebral hypoperfusion- induced neurodegeneration in rats
title_full Lipid peroxidation in chronic cerebral hypoperfusion- induced neurodegeneration in rats
title_fullStr Lipid peroxidation in chronic cerebral hypoperfusion- induced neurodegeneration in rats
title_full_unstemmed Lipid peroxidation in chronic cerebral hypoperfusion- induced neurodegeneration in rats
title_short Lipid peroxidation in chronic cerebral hypoperfusion- induced neurodegeneration in rats
title_sort lipid peroxidation in chronic cerebral hypoperfusion- induced neurodegeneration in rats
topic RC321 Neuroscience. Biological psychiatry. Neuropsychiatry
url http://irep.iium.edu.my/9981/
http://irep.iium.edu.my/9981/
http://irep.iium.edu.my/9981/1/download.pdf