Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate
In the present study, optimizing critical nutritional constituents was attempted as a primary strategy to improve biosurfactant production from Klebsiella pneumoniae WMF02 in liquid state fermentation utilizing sludge palm oil as a substrate. One-factor-at-a-time (OFAT) optimization was employed to...
| Main Authors: | , , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/9758/ http://irep.iium.edu.my/9758/1/wan.pdf |
| _version_ | 1848777154967371776 |
|---|---|
| author | Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir |
| author_facet | Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir |
| author_sort | Jamal, Parveen |
| building | IIUM Repository |
| collection | Online Access |
| description | In the present study, optimizing critical nutritional constituents was attempted as a primary strategy to improve biosurfactant production from Klebsiella pneumoniae WMF02 in liquid state fermentation utilizing sludge palm oil as a substrate. One-factor-at-a-time (OFAT) optimization was employed to evaluate the effects of sludge palm oil (SPO), sucrose, MgSO4, FeSO4, NaNO3 and K2HPO4 on surface tension reductivity. Sucrose was selected as a co-substrate over glucose in production media. The optimal levels of the aforementioned variables were (g/l) sucrose 5.0, MgSO4 0.4, FeSO4 0.3, NaNO3 2.0, and K2HPO4 4.0, with SPO concentration of 4% (v/v). The optimized medium shows surface tension reduction from 36.2 mN/m (non-optimized medium) to 25.70 mN/m. Preliminary biosurfactant identification indicated that the biosurfactant produced was phospholipids in nature. |
| first_indexed | 2025-11-14T14:41:29Z |
| format | Proceeding Paper |
| id | iium-9758 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:41:29Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-97582011-12-14T03:21:52Z http://irep.iium.edu.my/9758/ Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir TA164 Bioengineering TA170 Environmental engineering. Sustainable engineering TP248.13 Biotechnology In the present study, optimizing critical nutritional constituents was attempted as a primary strategy to improve biosurfactant production from Klebsiella pneumoniae WMF02 in liquid state fermentation utilizing sludge palm oil as a substrate. One-factor-at-a-time (OFAT) optimization was employed to evaluate the effects of sludge palm oil (SPO), sucrose, MgSO4, FeSO4, NaNO3 and K2HPO4 on surface tension reductivity. Sucrose was selected as a co-substrate over glucose in production media. The optimal levels of the aforementioned variables were (g/l) sucrose 5.0, MgSO4 0.4, FeSO4 0.3, NaNO3 2.0, and K2HPO4 4.0, with SPO concentration of 4% (v/v). The optimized medium shows surface tension reduction from 36.2 mN/m (non-optimized medium) to 25.70 mN/m. Preliminary biosurfactant identification indicated that the biosurfactant produced was phospholipids in nature. 2011 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/9758/1/wan.pdf Jamal, Parveen and Wan Nawawi, Wan Mohd Fazli and Alam, Md. Zahangir (2011) Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
| spellingShingle | TA164 Bioengineering TA170 Environmental engineering. Sustainable engineering TP248.13 Biotechnology Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate |
| title | Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate |
| title_full | Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate |
| title_fullStr | Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate |
| title_full_unstemmed | Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate |
| title_short | Optimum Medium Components for Biosurfactant Production by Klebsiella pneumoniae WMF02 Utilizing Sludge Palm Oil as a Substrate |
| title_sort | optimum medium components for biosurfactant production by klebsiella pneumoniae wmf02 utilizing sludge palm oil as a substrate |
| topic | TA164 Bioengineering TA170 Environmental engineering. Sustainable engineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/9758/ http://irep.iium.edu.my/9758/ http://irep.iium.edu.my/9758/1/wan.pdf |