Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate
In the present study, optimizing critical nutritional constituents was attempted as a primary strategy to improve biosurfactant production from Klebsiella pneumoniae WMF02 in liquid state fermentation utilizing sludge palm oil as a substrate. One-factor-at-a-time (OFAT) optimization was employed to...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
INSI Publications
2012
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/9757/ http://irep.iium.edu.my/9757/1/9757_Optimum%20medium%20components%20for%20biosurfactant.pdf http://irep.iium.edu.my/9757/2/9757_Optimum%20medium%20components%20for%20biosurfactant_SCOPUS.pdf |
| _version_ | 1848777154718859264 |
|---|---|
| author | Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir |
| author_facet | Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir |
| author_sort | Jamal, Parveen |
| building | IIUM Repository |
| collection | Online Access |
| description | In the present study, optimizing critical nutritional constituents was attempted as a primary strategy to improve biosurfactant production from Klebsiella pneumoniae WMF02 in liquid state fermentation utilizing sludge palm oil as a substrate. One-factor-at-a-time (OFAT) optimization was employed to evaluate the effects of sludge palm oil (SPO), sucrose, MgSO4, FeSO4, NaNO3 and K2HPO4 on surface tension reductivity. Sucrose was selected as a co-substrate over glucose in production media. The optimal levels of the aforementioned variables were (g/l) sucrose 5.0, MgSO4 0.4, FeSO4 0.3, NaNO3 2.0, and K2HPO4 4.0, with SPO concentration of 4% (v/v). The optimized medium shows surface tension reduction from 36.2 mN/m (non-optimized medium) to 25.70 mN/m. Preliminary biosurfactant identification indicated that the biosurfactant produced was phospholipids in nature. |
| first_indexed | 2025-11-14T14:41:29Z |
| format | Article |
| id | iium-9757 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T14:41:29Z |
| publishDate | 2012 |
| publisher | INSI Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-97572022-02-17T01:50:43Z http://irep.iium.edu.my/9757/ Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir TA164 Bioengineering TA170 Environmental engineering. Sustainable engineering TP248.13 Biotechnology In the present study, optimizing critical nutritional constituents was attempted as a primary strategy to improve biosurfactant production from Klebsiella pneumoniae WMF02 in liquid state fermentation utilizing sludge palm oil as a substrate. One-factor-at-a-time (OFAT) optimization was employed to evaluate the effects of sludge palm oil (SPO), sucrose, MgSO4, FeSO4, NaNO3 and K2HPO4 on surface tension reductivity. Sucrose was selected as a co-substrate over glucose in production media. The optimal levels of the aforementioned variables were (g/l) sucrose 5.0, MgSO4 0.4, FeSO4 0.3, NaNO3 2.0, and K2HPO4 4.0, with SPO concentration of 4% (v/v). The optimized medium shows surface tension reduction from 36.2 mN/m (non-optimized medium) to 25.70 mN/m. Preliminary biosurfactant identification indicated that the biosurfactant produced was phospholipids in nature. INSI Publications 2012-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/9757/1/9757_Optimum%20medium%20components%20for%20biosurfactant.pdf application/pdf en http://irep.iium.edu.my/9757/2/9757_Optimum%20medium%20components%20for%20biosurfactant_SCOPUS.pdf Jamal, Parveen and Wan Nawawi, Wan Mohd Fazli and Alam, Md. Zahangir (2012) Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate. Australian Journal of Basic and Applied Sciences, 6 (1). pp. 100-108. ISSN 1991-8178 http://www.insipub.com/ajbas/2012/January/100-108.pdf |
| spellingShingle | TA164 Bioengineering TA170 Environmental engineering. Sustainable engineering TP248.13 Biotechnology Jamal, Parveen Wan Nawawi, Wan Mohd Fazli Alam, Md. Zahangir Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate |
| title | Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate |
| title_full | Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate |
| title_fullStr | Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate |
| title_full_unstemmed | Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate |
| title_short | Optimum medium components for biosurfactant production by Klebsiella pneumoniae WMF02 utilizing sludge palm oil as a substrate |
| title_sort | optimum medium components for biosurfactant production by klebsiella pneumoniae wmf02 utilizing sludge palm oil as a substrate |
| topic | TA164 Bioengineering TA170 Environmental engineering. Sustainable engineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/9757/ http://irep.iium.edu.my/9757/ http://irep.iium.edu.my/9757/1/9757_Optimum%20medium%20components%20for%20biosurfactant.pdf http://irep.iium.edu.my/9757/2/9757_Optimum%20medium%20components%20for%20biosurfactant_SCOPUS.pdf |