Screening of Aspergillus for citric acid production from Palm Oil Mill Effluent

In this study, screening of potential microbes, especially Aspergillus, for citric acid production from Palm Oil Mill Effluent (POME) is carried out to improve the product yield. The fermentation of the raw material POME for the production of citric acid was conducted by the liquid state fermentatio...

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Main Authors: Jamal, Parveen, Alam, Md. Zahangir, Mohd Salleh, Mohammad Ramlan, Nadzir, M. M.
Format: Article
Language:English
Published: Asian Network for Scientific Information 2005
Subjects:
Online Access:http://irep.iium.edu.my/9734/
http://irep.iium.edu.my/9734/1/Screening_of_Aspergillus_for_Citric_Acid_Production_from_Palm_Oil_Mill_Effluent.pdf
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author Jamal, Parveen
Alam, Md. Zahangir
Mohd Salleh, Mohammad Ramlan
Nadzir, M. M.
author_facet Jamal, Parveen
Alam, Md. Zahangir
Mohd Salleh, Mohammad Ramlan
Nadzir, M. M.
author_sort Jamal, Parveen
building IIUM Repository
collection Online Access
description In this study, screening of potential microbes, especially Aspergillus, for citric acid production from Palm Oil Mill Effluent (POME) is carried out to improve the product yield. The fermentation of the raw material POME for the production of citric acid was conducted by the liquid state fermentation process. A total of ten strains of Aspergillus were selected for the screening test of which six strains were isolated from Sewage Treatment Plant Sludge (STP Sludge), purified and identified up to genus level and four strains of Aspergillus were from lab stock. All strains were screened under controlled fermentation conditions such as pH range of 2-3, temperature 30°C and agitation 150 rpm, using 1% (w/w) of substrate (POME), 2% (w/w) co-substrate (wheat flour) with inoculum size of 2% (106 spore mL 1). These strains were examined in terms of maximum citric acid production, biosolid production (TSS%) and Chemical Oxygen Demand (COD) removal. The strain Aspergillus (A103) produced the highest concentration of citric acid (0.28 g L 1), TSS (12.7 g L 1) and COD removal (72%) followed by A1020, A-SS106 and others on 2-4 days of fermentation.
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spelling iium-97342013-07-19T07:13:22Z http://irep.iium.edu.my/9734/ Screening of Aspergillus for citric acid production from Palm Oil Mill Effluent Jamal, Parveen Alam, Md. Zahangir Mohd Salleh, Mohammad Ramlan Nadzir, M. M. TA164 Bioengineering TA170 Environmental engineering. Sustainable engineering TP248.13 Biotechnology In this study, screening of potential microbes, especially Aspergillus, for citric acid production from Palm Oil Mill Effluent (POME) is carried out to improve the product yield. The fermentation of the raw material POME for the production of citric acid was conducted by the liquid state fermentation process. A total of ten strains of Aspergillus were selected for the screening test of which six strains were isolated from Sewage Treatment Plant Sludge (STP Sludge), purified and identified up to genus level and four strains of Aspergillus were from lab stock. All strains were screened under controlled fermentation conditions such as pH range of 2-3, temperature 30°C and agitation 150 rpm, using 1% (w/w) of substrate (POME), 2% (w/w) co-substrate (wheat flour) with inoculum size of 2% (106 spore mL 1). These strains were examined in terms of maximum citric acid production, biosolid production (TSS%) and Chemical Oxygen Demand (COD) removal. The strain Aspergillus (A103) produced the highest concentration of citric acid (0.28 g L 1), TSS (12.7 g L 1) and COD removal (72%) followed by A1020, A-SS106 and others on 2-4 days of fermentation. Asian Network for Scientific Information 2005 Article PeerReviewed application/pdf en http://irep.iium.edu.my/9734/1/Screening_of_Aspergillus_for_Citric_Acid_Production_from_Palm_Oil_Mill_Effluent.pdf Jamal, Parveen and Alam, Md. Zahangir and Mohd Salleh, Mohammad Ramlan and Nadzir, M. M. (2005) Screening of Aspergillus for citric acid production from Palm Oil Mill Effluent. Biotechnology, 4 (4). pp. 275-278. ISSN 1682-296X http://scialert.net/abstract/?doi=biotech.2005.275.278 10.3923/biotech.2005.275.278
spellingShingle TA164 Bioengineering
TA170 Environmental engineering. Sustainable engineering
TP248.13 Biotechnology
Jamal, Parveen
Alam, Md. Zahangir
Mohd Salleh, Mohammad Ramlan
Nadzir, M. M.
Screening of Aspergillus for citric acid production from Palm Oil Mill Effluent
title Screening of Aspergillus for citric acid production from Palm Oil Mill Effluent
title_full Screening of Aspergillus for citric acid production from Palm Oil Mill Effluent
title_fullStr Screening of Aspergillus for citric acid production from Palm Oil Mill Effluent
title_full_unstemmed Screening of Aspergillus for citric acid production from Palm Oil Mill Effluent
title_short Screening of Aspergillus for citric acid production from Palm Oil Mill Effluent
title_sort screening of aspergillus for citric acid production from palm oil mill effluent
topic TA164 Bioengineering
TA170 Environmental engineering. Sustainable engineering
TP248.13 Biotechnology
url http://irep.iium.edu.my/9734/
http://irep.iium.edu.my/9734/
http://irep.iium.edu.my/9734/
http://irep.iium.edu.my/9734/1/Screening_of_Aspergillus_for_Citric_Acid_Production_from_Palm_Oil_Mill_Effluent.pdf