Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion
A study was undertaken to observe the effect of D-glucose and L-glutamine and the combination of both nutrients at various concentrations towards the growth and insulin production by BRIN-BD11 cells. It was found that at a final concentration of 4 g/L D-glucose and 0.3 g/L L-glutamine in RPMI-1640 m...
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| Format: | Proceeding Paper |
| Language: | English |
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2009
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| Online Access: | http://irep.iium.edu.my/8909/ http://irep.iium.edu.my/8909/1/Seminar_on_Research_Findings_Series_No_2__2009__-_Prof_Ismail.pdf |
| _version_ | 1848777076352483328 |
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| author | Abdul Karim, Mohamed Ismail |
| author_facet | Abdul Karim, Mohamed Ismail |
| author_sort | Abdul Karim, Mohamed Ismail |
| building | IIUM Repository |
| collection | Online Access |
| description | A study was undertaken to observe the effect of D-glucose and L-glutamine and the combination of both nutrients at various concentrations towards the growth and insulin production by BRIN-BD11 cells. It was found that at a final concentration of 4 g/L D-glucose and 0.3 g/L L-glutamine in RPMI-1640 media gave maximum cell growth and insulin production of 1.75 x 106 cells/ml from 2.5 x 105 cells/ml and 100.77 µg/L, respectively. The result of this study indicates that D-glucose and L-glutamine promote good cell growth and insulin secretion of BRIN-BD11 cells as a step towards mass propagation of transplantable insulinoma cells and insulin production. |
| first_indexed | 2025-11-14T14:40:14Z |
| format | Proceeding Paper |
| id | iium-8909 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:40:14Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-89092012-04-04T06:54:39Z http://irep.iium.edu.my/8909/ Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion Abdul Karim, Mohamed Ismail TP248.13 Biotechnology A study was undertaken to observe the effect of D-glucose and L-glutamine and the combination of both nutrients at various concentrations towards the growth and insulin production by BRIN-BD11 cells. It was found that at a final concentration of 4 g/L D-glucose and 0.3 g/L L-glutamine in RPMI-1640 media gave maximum cell growth and insulin production of 1.75 x 106 cells/ml from 2.5 x 105 cells/ml and 100.77 µg/L, respectively. The result of this study indicates that D-glucose and L-glutamine promote good cell growth and insulin secretion of BRIN-BD11 cells as a step towards mass propagation of transplantable insulinoma cells and insulin production. 2009 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/8909/1/Seminar_on_Research_Findings_Series_No_2__2009__-_Prof_Ismail.pdf Abdul Karim, Mohamed Ismail (2009) Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion. In: Seminar on Research Findings Series No. 2, Kuala Lumpur, Malaysia. (Unpublished) |
| spellingShingle | TP248.13 Biotechnology Abdul Karim, Mohamed Ismail Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion |
| title | Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion |
| title_full | Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion |
| title_fullStr | Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion |
| title_full_unstemmed | Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion |
| title_short | Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion |
| title_sort | optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/8909/ http://irep.iium.edu.my/8909/1/Seminar_on_Research_Findings_Series_No_2__2009__-_Prof_Ismail.pdf |