Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
The effects of Cinnamomum burmannii and Tinospora crispa on differentiation and glucose uptake in 3T3-L1 adipocyte cells were studied. Cells were stimulated to differentiate with differentiation cocktail containing Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), 0.25 µM...
| Main Authors: | , , , , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/8306/ http://irep.iium.edu.my/8306/1/antidiabetic.pdf |
| _version_ | 1848776983599644672 |
|---|---|
| author | Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Solachuddin Jauhari, Arief Ichwan Kaderi, Mohd Arifin Darnis, Deny Susanti |
| author_facet | Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Solachuddin Jauhari, Arief Ichwan Kaderi, Mohd Arifin Darnis, Deny Susanti |
| author_sort | Bakhtiar, M. Taher |
| building | IIUM Repository |
| collection | Online Access |
| description | The effects of Cinnamomum burmannii and Tinospora crispa on differentiation and glucose uptake in 3T3-L1 adipocyte cells were studied. Cells were stimulated to differentiate with differentiation cocktail containing Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), 0.25 µM dexamethasone, 0.5 mM 3-isobutyl-1-methyl-xanthine and 167 µM insulin. 3T3-L1 preadipocytes were treated with various concentrations of the extracts in the presence of differentiation cocktail. Insulin was used as a positive control. The ability of the extracts to induce differentiation in preadipocytes was examined by measuring fat accumulation using oil red O staining. Our results showed that C. burmannii and T. crispa was statistically significant in inducing preadipocyte into mature adipocyte at dose of 0.1 mg/mL (p < 0.001) and 0.5 mg/mL (p < 0.05), respectively. Glucose uptake activity was investigated in differentiated adipocytes using a radioactive-labelled glucose by Liquid Scintillation Counter. The activities in the glucose uptake between untreated and treated cells showed that both plants were statistically significant compared metformin (1 mM), but not significant compared to sodium orthovanadate (5 mM). In conclusion, these results suggest that both plants were effective in inducing adipocyte differentiation and stimulate glucose uptake.
|
| first_indexed | 2025-11-14T14:38:45Z |
| format | Proceeding Paper |
| id | iium-8306 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:38:45Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-83062022-06-08T06:54:03Z http://irep.iium.edu.my/8306/ Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Solachuddin Jauhari, Arief Ichwan Kaderi, Mohd Arifin Darnis, Deny Susanti RS Pharmacy and materia medica The effects of Cinnamomum burmannii and Tinospora crispa on differentiation and glucose uptake in 3T3-L1 adipocyte cells were studied. Cells were stimulated to differentiate with differentiation cocktail containing Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), 0.25 µM dexamethasone, 0.5 mM 3-isobutyl-1-methyl-xanthine and 167 µM insulin. 3T3-L1 preadipocytes were treated with various concentrations of the extracts in the presence of differentiation cocktail. Insulin was used as a positive control. The ability of the extracts to induce differentiation in preadipocytes was examined by measuring fat accumulation using oil red O staining. Our results showed that C. burmannii and T. crispa was statistically significant in inducing preadipocyte into mature adipocyte at dose of 0.1 mg/mL (p < 0.001) and 0.5 mg/mL (p < 0.05), respectively. Glucose uptake activity was investigated in differentiated adipocytes using a radioactive-labelled glucose by Liquid Scintillation Counter. The activities in the glucose uptake between untreated and treated cells showed that both plants were statistically significant compared metformin (1 mM), but not significant compared to sodium orthovanadate (5 mM). In conclusion, these results suggest that both plants were effective in inducing adipocyte differentiation and stimulate glucose uptake. 2011 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/8306/1/antidiabetic.pdf Bakhtiar, M. Taher and Mohamed Amiroudine, Mohamed Zaffar Ali and Solachuddin Jauhari, Arief Ichwan and Kaderi, Mohd Arifin and Darnis, Deny Susanti (2011) Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes. In: International Conference on Natural products (ICNP 2011), 14-16 November 2011, Palm garden Resort. http://www.icnp2011.upm.edu.my/ |
| spellingShingle | RS Pharmacy and materia medica Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Solachuddin Jauhari, Arief Ichwan Kaderi, Mohd Arifin Darnis, Deny Susanti Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes |
| title | Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
|
| title_full | Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
|
| title_fullStr | Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
|
| title_full_unstemmed | Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
|
| title_short | Antidiabetic activities of Cinnamomum zeylanicum and Tinospora crispa on adipocytes
|
| title_sort | antidiabetic activities of cinnamomum zeylanicum and tinospora crispa on adipocytes |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/8306/ http://irep.iium.edu.my/8306/ http://irep.iium.edu.my/8306/1/antidiabetic.pdf |