Thymoquinone content in marketed black seed oil in Malaysia

Thymoquinone (TQ) is the major active compound in black seed oil (BSO). Many pharmacological effects of TQ, such as anti-inflammatory, hypoglycemic, antioxidant, immune stimulator, and anticancer, have been reported. TQ can be considered as a biomarker for BSO, but its content in the commercial pr...

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Main Authors: Alkhatib, Hamzeh, Mawazi, SaeidMezail, Al-Mahmood, SinanMohammed Abdullah, Zaiter, Ahmad, Doolaanea, Abd Almonem
Format: Article
Language:English
Published: Wolters Kluwer 2020
Subjects:
Online Access:http://irep.iium.edu.my/82819/
http://irep.iium.edu.my/82819/1/Thymoquinone%20content%20in%20marketed%20black%20seed%20oil%20in%20Malaysia%20Alkhatib%20H%2C%20Mawazi%20SM%2C%20Al-Mahmood%20SM%2C%20Zaiter%20A%2C%20Doolaanea%20AA%20-%20J%20Pharm%20Bioall%20Sci.pdf
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author Alkhatib, Hamzeh
Mawazi, SaeidMezail
Al-Mahmood, SinanMohammed Abdullah
Zaiter, Ahmad
Doolaanea, Abd Almonem
author_facet Alkhatib, Hamzeh
Mawazi, SaeidMezail
Al-Mahmood, SinanMohammed Abdullah
Zaiter, Ahmad
Doolaanea, Abd Almonem
author_sort Alkhatib, Hamzeh
building IIUM Repository
collection Online Access
description Thymoquinone (TQ) is the major active compound in black seed oil (BSO). Many pharmacological effects of TQ, such as anti-inflammatory, hypoglycemic, antioxidant, immune stimulator, and anticancer, have been reported. TQ can be considered as a biomarker for BSO, but its content in the commercial products is rarely reported. TQ content varies based on the oil source and extraction method. This study aimed to quantify the TQ content in the commercial BSO products in Malaysia and to evaluate whether the products can be used as a source of TQ for therapeutic benefits. TQ was quantified using an established high-performance liquid chromatography (HPLC) method. TQ human equivalent dose (HED) was calculated based on reported animal studies from literature, and theoretical BSO amount containing the TQ dose was calculated based on the HPLC analysis. TQ content in the commercial BSO products ranged from 0.07% wt/wt to 1.88% wt/wt. The product with the highest TQ concentration is approximately 27-fold higher than the product with the lowest TQ concentration. Consequently, theoretical BSO amounts needed for specific diseases varied and some products cannot provide practical amount of TQ. This study recommends the regulation of TQ content in BSO and suggests that the BSO might be fortified with extra TQ to be effectively used in some diseases.
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institution International Islamic University Malaysia
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spelling iium-828192021-01-05T03:00:15Z http://irep.iium.edu.my/82819/ Thymoquinone content in marketed black seed oil in Malaysia Alkhatib, Hamzeh Mawazi, SaeidMezail Al-Mahmood, SinanMohammed Abdullah Zaiter, Ahmad Doolaanea, Abd Almonem RS Pharmacy and materia medica Thymoquinone (TQ) is the major active compound in black seed oil (BSO). Many pharmacological effects of TQ, such as anti-inflammatory, hypoglycemic, antioxidant, immune stimulator, and anticancer, have been reported. TQ can be considered as a biomarker for BSO, but its content in the commercial products is rarely reported. TQ content varies based on the oil source and extraction method. This study aimed to quantify the TQ content in the commercial BSO products in Malaysia and to evaluate whether the products can be used as a source of TQ for therapeutic benefits. TQ was quantified using an established high-performance liquid chromatography (HPLC) method. TQ human equivalent dose (HED) was calculated based on reported animal studies from literature, and theoretical BSO amount containing the TQ dose was calculated based on the HPLC analysis. TQ content in the commercial BSO products ranged from 0.07% wt/wt to 1.88% wt/wt. The product with the highest TQ concentration is approximately 27-fold higher than the product with the lowest TQ concentration. Consequently, theoretical BSO amounts needed for specific diseases varied and some products cannot provide practical amount of TQ. This study recommends the regulation of TQ content in BSO and suggests that the BSO might be fortified with extra TQ to be effectively used in some diseases. Wolters Kluwer 2020-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82819/1/Thymoquinone%20content%20in%20marketed%20black%20seed%20oil%20in%20Malaysia%20Alkhatib%20H%2C%20Mawazi%20SM%2C%20Al-Mahmood%20SM%2C%20Zaiter%20A%2C%20Doolaanea%20AA%20-%20J%20Pharm%20Bioall%20Sci.pdf Alkhatib, Hamzeh and Mawazi, SaeidMezail and Al-Mahmood, SinanMohammed Abdullah and Zaiter, Ahmad and Doolaanea, Abd Almonem (2020) Thymoquinone content in marketed black seed oil in Malaysia. Journal of Pharmacy And Bioallied Sciences, 12 (3). pp. 284-288. ISSN 0975-7406 http://doi.org/10.4103/jpbs.JPBS_208_20 doi:10.4103/jpbs.JPBS_208_20
spellingShingle RS Pharmacy and materia medica
Alkhatib, Hamzeh
Mawazi, SaeidMezail
Al-Mahmood, SinanMohammed Abdullah
Zaiter, Ahmad
Doolaanea, Abd Almonem
Thymoquinone content in marketed black seed oil in Malaysia
title Thymoquinone content in marketed black seed oil in Malaysia
title_full Thymoquinone content in marketed black seed oil in Malaysia
title_fullStr Thymoquinone content in marketed black seed oil in Malaysia
title_full_unstemmed Thymoquinone content in marketed black seed oil in Malaysia
title_short Thymoquinone content in marketed black seed oil in Malaysia
title_sort thymoquinone content in marketed black seed oil in malaysia
topic RS Pharmacy and materia medica
url http://irep.iium.edu.my/82819/
http://irep.iium.edu.my/82819/
http://irep.iium.edu.my/82819/
http://irep.iium.edu.my/82819/1/Thymoquinone%20content%20in%20marketed%20black%20seed%20oil%20in%20Malaysia%20Alkhatib%20H%2C%20Mawazi%20SM%2C%20Al-Mahmood%20SM%2C%20Zaiter%20A%2C%20Doolaanea%20AA%20-%20J%20Pharm%20Bioall%20Sci.pdf