Identification of haptoglobin as a potential biomarker in young adults with acute myocardial infarction by proteomic analysis
Background: Acute myocardial infarction (AMI) molecular research in young adults is still limited. The aim of this study is to identify AMI proteomic biomarker(s) in young adults. Methods: This study comprised of two phases namely discovery and verification. In the discovery phase, proteins in the p...
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| Format: | Article |
| Language: | English English |
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Penerbit Universiti Sains Malaysia
2020
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| Online Access: | http://irep.iium.edu.my/82650/ http://irep.iium.edu.my/82650/2/82650_Identification%20of%20haptoglobin%20as%20a%20potential%20biomarkers_ft.pdf http://irep.iium.edu.my/82650/1/82650_Identification%20of%20haptoglobin%20as%20a%20potential%20biomarkers_scopus.pdf |
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| author | Mohamed Bakrim, Norbaiyah Mohd. Shah, Aida Nur Sharini Talib, Norlelawati A. Ab Rahman, Jamalludin Abdullah, Aszrin |
| author_facet | Mohamed Bakrim, Norbaiyah Mohd. Shah, Aida Nur Sharini Talib, Norlelawati A. Ab Rahman, Jamalludin Abdullah, Aszrin |
| author_sort | Mohamed Bakrim, Norbaiyah |
| building | IIUM Repository |
| collection | Online Access |
| description | Background: Acute myocardial infarction (AMI) molecular research in young adults is still limited. The aim of this study is to identify AMI proteomic biomarker(s) in young adults. Methods: This study comprised of two phases namely discovery and verification. In the discovery phase, proteins in the pooled plasma samples from young male adults between 18 and 45 years (10 AMI patients and 10 controls) were separated using two-dimensional electrophoresis. The protein spots that were expressed differently in the AMI patients were identified via matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. The plasma concentrations of these proteins were quantified using enzyme-linked immunosorbent assay during the verification phase (40 AMI patients and 80 controls). Results: Haptoglobin (Hp), apolipoprotein AI (Apo AI) and apolipoprotein AIV (Apo AIV) were up-regulated in the discovery phase. In the verification phase, the plasma concentration of Hp was significantly higher in AMI patients than the controls (P < 0.001). Logistic regression showed an association between Hp and AMI in young adults (odds ratio [OR] = 1.016, 95% CI: 1.002– 1.030, P = 0.025) independent of other AMI risk factors. Hp was significantly correlated with high sensitivity C-reactive protein (hs-CRP) (r = 0.424, P < 0.001). Conclusion: In young adults with AMI, plasma Hp concentrations were elevated and it is independently associated with AMI. A positive correlation with hs-CRP suggests Hp could be a potential biomarker of AMI in young adults. |
| first_indexed | 2025-11-14T17:55:09Z |
| format | Article |
| id | iium-82650 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:55:09Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-826502021-02-19T06:53:08Z http://irep.iium.edu.my/82650/ Identification of haptoglobin as a potential biomarker in young adults with acute myocardial infarction by proteomic analysis Mohamed Bakrim, Norbaiyah Mohd. Shah, Aida Nur Sharini Talib, Norlelawati A. Ab Rahman, Jamalludin Abdullah, Aszrin QH301 Biology R Medicine (General) Background: Acute myocardial infarction (AMI) molecular research in young adults is still limited. The aim of this study is to identify AMI proteomic biomarker(s) in young adults. Methods: This study comprised of two phases namely discovery and verification. In the discovery phase, proteins in the pooled plasma samples from young male adults between 18 and 45 years (10 AMI patients and 10 controls) were separated using two-dimensional electrophoresis. The protein spots that were expressed differently in the AMI patients were identified via matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. The plasma concentrations of these proteins were quantified using enzyme-linked immunosorbent assay during the verification phase (40 AMI patients and 80 controls). Results: Haptoglobin (Hp), apolipoprotein AI (Apo AI) and apolipoprotein AIV (Apo AIV) were up-regulated in the discovery phase. In the verification phase, the plasma concentration of Hp was significantly higher in AMI patients than the controls (P < 0.001). Logistic regression showed an association between Hp and AMI in young adults (odds ratio [OR] = 1.016, 95% CI: 1.002– 1.030, P = 0.025) independent of other AMI risk factors. Hp was significantly correlated with high sensitivity C-reactive protein (hs-CRP) (r = 0.424, P < 0.001). Conclusion: In young adults with AMI, plasma Hp concentrations were elevated and it is independently associated with AMI. A positive correlation with hs-CRP suggests Hp could be a potential biomarker of AMI in young adults. Penerbit Universiti Sains Malaysia 2020-04-30 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82650/2/82650_Identification%20of%20haptoglobin%20as%20a%20potential%20biomarkers_ft.pdf application/pdf en http://irep.iium.edu.my/82650/1/82650_Identification%20of%20haptoglobin%20as%20a%20potential%20biomarkers_scopus.pdf Mohamed Bakrim, Norbaiyah and Mohd. Shah, Aida Nur Sharini and Talib, Norlelawati A. and Ab Rahman, Jamalludin and Abdullah, Aszrin (2020) Identification of haptoglobin as a potential biomarker in young adults with acute myocardial infarction by proteomic analysis. Malaysian Journal of Medical Sciences, 27 (2). pp. 64-76. ISSN 1394-195X E-ISSN 2180-4303 http://www.mjms.usm.my/newsletter/mjms27022020.html 10.21315/mjms2020.27.2.8 |
| spellingShingle | QH301 Biology R Medicine (General) Mohamed Bakrim, Norbaiyah Mohd. Shah, Aida Nur Sharini Talib, Norlelawati A. Ab Rahman, Jamalludin Abdullah, Aszrin Identification of haptoglobin as a potential biomarker in young adults with acute myocardial infarction by proteomic analysis |
| title | Identification of haptoglobin as a potential biomarker in young adults with acute myocardial infarction by proteomic analysis |
| title_full | Identification of haptoglobin as a potential biomarker in young adults with acute myocardial infarction by proteomic analysis |
| title_fullStr | Identification of haptoglobin as a potential biomarker in young adults with acute myocardial infarction by proteomic analysis |
| title_full_unstemmed | Identification of haptoglobin as a potential biomarker in young adults with acute myocardial infarction by proteomic analysis |
| title_short | Identification of haptoglobin as a potential biomarker in young adults with acute myocardial infarction by proteomic analysis |
| title_sort | identification of haptoglobin as a potential biomarker in young adults with acute myocardial infarction by proteomic analysis |
| topic | QH301 Biology R Medicine (General) |
| url | http://irep.iium.edu.my/82650/ http://irep.iium.edu.my/82650/ http://irep.iium.edu.my/82650/ http://irep.iium.edu.my/82650/2/82650_Identification%20of%20haptoglobin%20as%20a%20potential%20biomarkers_ft.pdf http://irep.iium.edu.my/82650/1/82650_Identification%20of%20haptoglobin%20as%20a%20potential%20biomarkers_scopus.pdf |