Effect of dentin pre-treatment on smear layer thickness. an ultrastructural study

The aim of this study was to investigate the morphological changes of etched dentin by sodium hypochlorite (NaOCl) pre-treatment method applied for different durations and smear layer (SL) thickness. Sixty human molars were used in this study and sectioned at the coronal part to expose a flat dentin...

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Main Authors: Mahdan, Mohd. Haidil Akmal, Samani, Nur Sarahniza, Mohd Ridzuan, Syakirah, Maideen, Mohd. Maizam
Format: Article
Language:English
English
Published: Microscopy Society of Malaysia 2020
Subjects:
Online Access:http://irep.iium.edu.my/82614/
http://irep.iium.edu.my/82614/2/82614_Effect%20of%20dentin%20pre-treatment%20on%20smear%20layer%20thickness_ft.pdf
http://irep.iium.edu.my/82614/1/82614_Effect%20of%20dentin%20pre-treatment%20on%20smear%20layer%20thickness_scopus.pdf
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author Mahdan, Mohd. Haidil Akmal
Samani, Nur Sarahniza
Mohd Ridzuan, Syakirah
Maideen, Mohd. Maizam
author_facet Mahdan, Mohd. Haidil Akmal
Samani, Nur Sarahniza
Mohd Ridzuan, Syakirah
Maideen, Mohd. Maizam
author_sort Mahdan, Mohd. Haidil Akmal
building IIUM Repository
collection Online Access
description The aim of this study was to investigate the morphological changes of etched dentin by sodium hypochlorite (NaOCl) pre-treatment method applied for different durations and smear layer (SL) thickness. Sixty human molars were used in this study and sectioned at the coronal part to expose a flat dentin disc. They were ground with #180-or #600-grit silicon carbide papers, to produce thick and thin SL respectively. All specimens were assigned into 6 groups; G1: control; G2: Acid Etch (AE); G3, G4, G5 and G6: AE + 10% NaOCl for 15, 30, 60, and 120s, respectively. Specimens were observed under a Scanning Electron Microscope and the photomicrographs were classified according to the following scores: 0: presence of SL; 1: No smear layer + non-altered collagen fibrils; 2: No smear layer + slightly altered collagen fibrils; 3: No smear layer + severely altered collagen fibrils; and 4: No smear layer and absence of collagen fibrils. Kruskal Wallis showed all groups treated with NaOCl solution were significantly different, and alteration regarding the collagen fibrils network. The higher the duration of NaOCl pre-treatment, the more alteration of collagen fibrils occured.
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spelling iium-826142021-02-17T06:18:41Z http://irep.iium.edu.my/82614/ Effect of dentin pre-treatment on smear layer thickness. an ultrastructural study Mahdan, Mohd. Haidil Akmal Samani, Nur Sarahniza Mohd Ridzuan, Syakirah Maideen, Mohd. Maizam R Medicine (General) RK Dentistry The aim of this study was to investigate the morphological changes of etched dentin by sodium hypochlorite (NaOCl) pre-treatment method applied for different durations and smear layer (SL) thickness. Sixty human molars were used in this study and sectioned at the coronal part to expose a flat dentin disc. They were ground with #180-or #600-grit silicon carbide papers, to produce thick and thin SL respectively. All specimens were assigned into 6 groups; G1: control; G2: Acid Etch (AE); G3, G4, G5 and G6: AE + 10% NaOCl for 15, 30, 60, and 120s, respectively. Specimens were observed under a Scanning Electron Microscope and the photomicrographs were classified according to the following scores: 0: presence of SL; 1: No smear layer + non-altered collagen fibrils; 2: No smear layer + slightly altered collagen fibrils; 3: No smear layer + severely altered collagen fibrils; and 4: No smear layer and absence of collagen fibrils. Kruskal Wallis showed all groups treated with NaOCl solution were significantly different, and alteration regarding the collagen fibrils network. The higher the duration of NaOCl pre-treatment, the more alteration of collagen fibrils occured. Microscopy Society of Malaysia 2020 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82614/2/82614_Effect%20of%20dentin%20pre-treatment%20on%20smear%20layer%20thickness_ft.pdf application/pdf en http://irep.iium.edu.my/82614/1/82614_Effect%20of%20dentin%20pre-treatment%20on%20smear%20layer%20thickness_scopus.pdf Mahdan, Mohd. Haidil Akmal and Samani, Nur Sarahniza and Mohd Ridzuan, Syakirah and Maideen, Mohd. Maizam (2020) Effect of dentin pre-treatment on smear layer thickness. an ultrastructural study. Malaysian Journal of Microscopy, 16 (1). pp. 94-104. ISSN 1823-7010
spellingShingle R Medicine (General)
RK Dentistry
Mahdan, Mohd. Haidil Akmal
Samani, Nur Sarahniza
Mohd Ridzuan, Syakirah
Maideen, Mohd. Maizam
Effect of dentin pre-treatment on smear layer thickness. an ultrastructural study
title Effect of dentin pre-treatment on smear layer thickness. an ultrastructural study
title_full Effect of dentin pre-treatment on smear layer thickness. an ultrastructural study
title_fullStr Effect of dentin pre-treatment on smear layer thickness. an ultrastructural study
title_full_unstemmed Effect of dentin pre-treatment on smear layer thickness. an ultrastructural study
title_short Effect of dentin pre-treatment on smear layer thickness. an ultrastructural study
title_sort effect of dentin pre-treatment on smear layer thickness. an ultrastructural study
topic R Medicine (General)
RK Dentistry
url http://irep.iium.edu.my/82614/
http://irep.iium.edu.my/82614/2/82614_Effect%20of%20dentin%20pre-treatment%20on%20smear%20layer%20thickness_ft.pdf
http://irep.iium.edu.my/82614/1/82614_Effect%20of%20dentin%20pre-treatment%20on%20smear%20layer%20thickness_scopus.pdf