Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2
Fruit bromelain is a proteolytic enzyme harbouring cysteine catalytic residue found abundantly in pineapple fruit. The expression of cysteine proteases is usually regulated during fruit ripening. In the present study, we aimed to study the expression and proteolytic activity level of fruit bromelain...
| Main Authors: | , , |
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| Format: | Book Chapter |
| Language: | English English |
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Trans Tech Publications Ltd
2020
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| Online Access: | http://irep.iium.edu.my/82591/ http://irep.iium.edu.my/82591/1/82591_Gene%20expression%20analysis%20of%20fruit%20bromelain_scopus.pdf http://irep.iium.edu.my/82591/7/82591_Gene%20expression%20analysis%20of%20fruit_MYRA.pdf |
| _version_ | 1848789325545734144 |
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| author | Pang, Wei Cheng Ramli, Aizi Nor Mazila Abdul Hamid, Azzmer Azzar |
| author_facet | Pang, Wei Cheng Ramli, Aizi Nor Mazila Abdul Hamid, Azzmer Azzar |
| author_sort | Pang, Wei Cheng |
| building | IIUM Repository |
| collection | Online Access |
| description | Fruit bromelain is a proteolytic enzyme harbouring cysteine catalytic residue found abundantly in pineapple fruit. The expression of cysteine proteases is usually regulated during fruit ripening. In the present study, we aimed to study the expression and proteolytic activity level of fruit bromelain during the ripening stage of A. comosus cultivar MD 2. The gene expression of fruit bromelain was investigated via relative gene expression analysis using qPCR while the proteolytic activity of fruit bromelain was analysed via enzymatic assay using casein as a substrate. The qPCR analysis revealed that the expression of fruit bromelain was down-regulated 10-fold in ripe pineapple fruits. Besides that, the unripe pineapple fruits [1.9101 ± 0.0831 U/mL] had a higher proteolytic activity than the ripe MD 2 pineapple fruits [1.1333 ± 0.0896 U/mL]. This result showed that the function of fruit bromelain may be related to the protection of young pineapple fruits during the fruit development stage. |
| first_indexed | 2025-11-14T17:54:56Z |
| format | Book Chapter |
| id | iium-82591 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:54:56Z |
| publishDate | 2020 |
| publisher | Trans Tech Publications Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-825912021-01-19T03:47:24Z http://irep.iium.edu.my/82591/ Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2 Pang, Wei Cheng Ramli, Aizi Nor Mazila Abdul Hamid, Azzmer Azzar TP248.13 Biotechnology Fruit bromelain is a proteolytic enzyme harbouring cysteine catalytic residue found abundantly in pineapple fruit. The expression of cysteine proteases is usually regulated during fruit ripening. In the present study, we aimed to study the expression and proteolytic activity level of fruit bromelain during the ripening stage of A. comosus cultivar MD 2. The gene expression of fruit bromelain was investigated via relative gene expression analysis using qPCR while the proteolytic activity of fruit bromelain was analysed via enzymatic assay using casein as a substrate. The qPCR analysis revealed that the expression of fruit bromelain was down-regulated 10-fold in ripe pineapple fruits. Besides that, the unripe pineapple fruits [1.9101 ± 0.0831 U/mL] had a higher proteolytic activity than the ripe MD 2 pineapple fruits [1.1333 ± 0.0896 U/mL]. This result showed that the function of fruit bromelain may be related to the protection of young pineapple fruits during the fruit development stage. Trans Tech Publications Ltd 2020-03-12 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/82591/1/82591_Gene%20expression%20analysis%20of%20fruit%20bromelain_scopus.pdf application/pdf en http://irep.iium.edu.my/82591/7/82591_Gene%20expression%20analysis%20of%20fruit_MYRA.pdf Pang, Wei Cheng and Ramli, Aizi Nor Mazila and Abdul Hamid, Azzmer Azzar (2020) Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2. In: Materials Science Forum. Trans Tech Publications Ltd, Kuantan, Pahang, pp. 209-214. ISBN 978-303571664-1 https://www.scientific.net/MSF.981.209 10.4028/www.scientific.net/MSF.981.209 |
| spellingShingle | TP248.13 Biotechnology Pang, Wei Cheng Ramli, Aizi Nor Mazila Abdul Hamid, Azzmer Azzar Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2 |
| title | Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2 |
| title_full | Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2 |
| title_fullStr | Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2 |
| title_full_unstemmed | Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2 |
| title_short | Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2 |
| title_sort | gene expression analysis of fruit bromelain in ripening of ananas comosus cultivar md 2 |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/82591/ http://irep.iium.edu.my/82591/ http://irep.iium.edu.my/82591/ http://irep.iium.edu.my/82591/1/82591_Gene%20expression%20analysis%20of%20fruit%20bromelain_scopus.pdf http://irep.iium.edu.my/82591/7/82591_Gene%20expression%20analysis%20of%20fruit_MYRA.pdf |