Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL...
| Main Authors: | , , , , , |
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| Format: | Book Chapter |
| Language: | English English |
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Trans Tech Publications Ltd
2020
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| Online Access: | http://irep.iium.edu.my/82587/ http://irep.iium.edu.my/82587/1/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_scopus.pdf http://irep.iium.edu.my/82587/7/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_ft.pdf |
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| author | Bakar, Nur Husnina Iffah Lee, Thong Chuan Mohd-Hairul, Ab Rahim Ahmad Raus, Raha Basri, Dayang Fredalina Adzahar, Noor Suhana |
| author_facet | Bakar, Nur Husnina Iffah Lee, Thong Chuan Mohd-Hairul, Ab Rahim Ahmad Raus, Raha Basri, Dayang Fredalina Adzahar, Noor Suhana |
| author_sort | Bakar, Nur Husnina Iffah |
| building | IIUM Repository |
| collection | Online Access |
| description | A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1- dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract. |
| first_indexed | 2025-11-14T17:54:55Z |
| format | Book Chapter |
| id | iium-82587 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:54:55Z |
| publishDate | 2020 |
| publisher | Trans Tech Publications Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-825872020-09-02T07:55:50Z http://irep.iium.edu.my/82587/ Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis Bakar, Nur Husnina Iffah Lee, Thong Chuan Mohd-Hairul, Ab Rahim Ahmad Raus, Raha Basri, Dayang Fredalina Adzahar, Noor Suhana TP248.13 Biotechnology A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1- dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract. Trans Tech Publications Ltd 2020-03-27 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/82587/1/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_scopus.pdf application/pdf en http://irep.iium.edu.my/82587/7/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_ft.pdf Bakar, Nur Husnina Iffah and Lee, Thong Chuan and Mohd-Hairul, Ab Rahim and Ahmad Raus, Raha and Basri, Dayang Fredalina and Adzahar, Noor Suhana (2020) Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis. In: Materials Science Forum. Trans Tech Publications Ltd, Kuantan, Malaysia, pp. 265-270. ISBN 978-303571664-1 https://www.scientific.net/MSF.981.265 10.4028/www.scientific.net/MSF.981.265 |
| spellingShingle | TP248.13 Biotechnology Bakar, Nur Husnina Iffah Lee, Thong Chuan Mohd-Hairul, Ab Rahim Ahmad Raus, Raha Basri, Dayang Fredalina Adzahar, Noor Suhana Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title | Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title_full | Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title_fullStr | Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title_full_unstemmed | Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title_short | Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis |
| title_sort | real-time cytotoxicity assay of water extracts hibiscus rosa-sinensis |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/82587/ http://irep.iium.edu.my/82587/ http://irep.iium.edu.my/82587/ http://irep.iium.edu.my/82587/1/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_scopus.pdf http://irep.iium.edu.my/82587/7/82587_Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis_ft.pdf |