Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay
This study aimed to investigate the cytotoxicity of Lagerstroemia speciosa (L.) leaves crude extract. It has been reported to show various bioactivities. The phytochemical screening of the extract has been carried qualitatively. The cytotoxic effect was determined through in vitro MTT assay of M...
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| Format: | Article |
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International Journal of Drug Delivery Technology
2020
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| Online Access: | http://irep.iium.edu.my/82575/ http://irep.iium.edu.my/82575/7/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa%20%28L.%29%20leaves%20extract%20by%20MCF-7%20cell%20line%20and%20brine%20shrimp%20lethality%20bioassay.pdf http://irep.iium.edu.my/82575/13/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa.pdf |
| _version_ | 1848789322844602368 |
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| author | Azad, Abul Kalam Helal Uddin, A.B.M. |
| author_facet | Azad, Abul Kalam Helal Uddin, A.B.M. |
| author_sort | Azad, Abul Kalam |
| building | IIUM Repository |
| collection | Online Access |
| description | This study aimed to investigate the cytotoxicity of Lagerstroemia speciosa (L.) leaves crude extract. It has been reported to
show various bioactivities. The phytochemical screening of the extract has been carried qualitatively. The cytotoxic effect
was determined through in vitro MTT assay of MCF-7 cell line, and brine shrimp lethality bioassay. The presence or absence
of alkaloid, carbohydrate, glycoside, saponin, terpene, steroid, phenol, and flavonoid in the extract was determined through
the qualitative tests. The extract showed cell viability of 100% (1.95–3.9 μL/mL), 96% (1.95 μL/mL), ≈ 95% (3.9–15.62 μL/
mL) and 88% (250 μL/mL) while the mortality of brine shrimp nauplii was from 5% to 10% (7.8 – 125 μL/mL) respectively.
For both assays, DMSO of 1 & 0.1% were used as vehicle controls, while the potassium dichromate as the positive control
for the brine shrimp only. These results proved the leaves extract to be non-toxic. |
| first_indexed | 2025-11-14T17:54:53Z |
| format | Article |
| id | iium-82575 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:54:53Z |
| publishDate | 2020 |
| publisher | International Journal of Drug Delivery Technology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-825752022-11-07T02:24:41Z http://irep.iium.edu.my/82575/ Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay Azad, Abul Kalam Helal Uddin, A.B.M. QD Chemistry RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry This study aimed to investigate the cytotoxicity of Lagerstroemia speciosa (L.) leaves crude extract. It has been reported to show various bioactivities. The phytochemical screening of the extract has been carried qualitatively. The cytotoxic effect was determined through in vitro MTT assay of MCF-7 cell line, and brine shrimp lethality bioassay. The presence or absence of alkaloid, carbohydrate, glycoside, saponin, terpene, steroid, phenol, and flavonoid in the extract was determined through the qualitative tests. The extract showed cell viability of 100% (1.95–3.9 μL/mL), 96% (1.95 μL/mL), ≈ 95% (3.9–15.62 μL/ mL) and 88% (250 μL/mL) while the mortality of brine shrimp nauplii was from 5% to 10% (7.8 – 125 μL/mL) respectively. For both assays, DMSO of 1 & 0.1% were used as vehicle controls, while the potassium dichromate as the positive control for the brine shrimp only. These results proved the leaves extract to be non-toxic. International Journal of Drug Delivery Technology 2020-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82575/7/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa%20%28L.%29%20leaves%20extract%20by%20MCF-7%20cell%20line%20and%20brine%20shrimp%20lethality%20bioassay.pdf application/pdf en http://irep.iium.edu.my/82575/13/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa.pdf Azad, Abul Kalam and Helal Uddin, A.B.M. (2020) Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay. International Journal of Drug Delivery Technology, 10 (1). pp. 40-44. ISSN 0975-4415 https://ijddt.com/volume10issue1/ 10.25258/ijddt.10.1.6 |
| spellingShingle | QD Chemistry RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Azad, Abul Kalam Helal Uddin, A.B.M. Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay |
| title | Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa
(L.) leaves extract by MCF-7 cell line and brine shrimp lethality
bioassay |
| title_full | Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa
(L.) leaves extract by MCF-7 cell line and brine shrimp lethality
bioassay |
| title_fullStr | Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa
(L.) leaves extract by MCF-7 cell line and brine shrimp lethality
bioassay |
| title_full_unstemmed | Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa
(L.) leaves extract by MCF-7 cell line and brine shrimp lethality
bioassay |
| title_short | Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa
(L.) leaves extract by MCF-7 cell line and brine shrimp lethality
bioassay |
| title_sort | phytochemical and cytotoxicity evaluation of lagerstroemia speciosa
(l.) leaves extract by mcf-7 cell line and brine shrimp lethality
bioassay |
| topic | QD Chemistry RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry |
| url | http://irep.iium.edu.my/82575/ http://irep.iium.edu.my/82575/ http://irep.iium.edu.my/82575/ http://irep.iium.edu.my/82575/7/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa%20%28L.%29%20leaves%20extract%20by%20MCF-7%20cell%20line%20and%20brine%20shrimp%20lethality%20bioassay.pdf http://irep.iium.edu.my/82575/13/82575_Phytochemical%20and%20cytotoxicity%20evaluation%20of%20lagerstroemia%20speciosa.pdf |