In vitro evaluation of poly (lactic acid-co-glycolic acid)/ atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration
The current study evaluated the 3D culture of the PLGA incorporated with atelocollagen and fibrin seeded with the annulus cells of intervertebral disc. The evaluation was performed in three consecutive weeks observing at the morphology, compression modulus, histology, immunohistology, sulphated glyc...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Society for Biomaterials and Artificial Organs - India
2020
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/82477/ http://irep.iium.edu.my/82477/19/82477_In%20vitro%20evaluation%20of%20poly_Scopus.pdf http://irep.iium.edu.my/82477/25/82477_In%20vitro%20evaluation%20of%20poly.pdf |
| _version_ | 1848789304920244224 |
|---|---|
| author | Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Zulkifly, Ahmad Hafiz Sha'ban, Munirah |
| author_facet | Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Zulkifly, Ahmad Hafiz Sha'ban, Munirah |
| author_sort | Mohamad, Mohd Yusof |
| building | IIUM Repository |
| collection | Online Access |
| description | The current study evaluated the 3D culture of the PLGA incorporated with atelocollagen and fibrin seeded with the annulus cells of intervertebral disc. The evaluation was performed in three consecutive weeks observing at the morphology, compression modulus, histology, immunohistology, sulphated glycosaminoglycan (SGAG) and DNA content. A comparison was made between the AF seeded onto the PLGA (PLGA-AF), PLGA with atelocollagen (PAAF), PLGA with fibrin (PF-AF) and PLGA with both atelocollagen and fibrin (PAF-AF). The constructs displayed glistening morphology with high wet weight and cellular viability by week 3. The PAF-AF and PA-AF demonstrated significant compression modulus. The presence of collagen type I were detected in all the constructs. The histological findings showed extensive AF cellular distribution. Significant quantitative sGAG was detected for the PAF-AF constructs but with no significant differences on the DNA content. Overall, the PAF-AF constructs support the cellular proliferation of the AF |
| first_indexed | 2025-11-14T17:54:36Z |
| format | Article |
| id | iium-82477 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:54:36Z |
| publishDate | 2020 |
| publisher | Society for Biomaterials and Artificial Organs - India |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-824772021-02-10T03:20:39Z http://irep.iium.edu.my/82477/ In vitro evaluation of poly (lactic acid-co-glycolic acid)/ atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Zulkifly, Ahmad Hafiz Sha'ban, Munirah R Medicine (General) The current study evaluated the 3D culture of the PLGA incorporated with atelocollagen and fibrin seeded with the annulus cells of intervertebral disc. The evaluation was performed in three consecutive weeks observing at the morphology, compression modulus, histology, immunohistology, sulphated glycosaminoglycan (SGAG) and DNA content. A comparison was made between the AF seeded onto the PLGA (PLGA-AF), PLGA with atelocollagen (PAAF), PLGA with fibrin (PF-AF) and PLGA with both atelocollagen and fibrin (PAF-AF). The constructs displayed glistening morphology with high wet weight and cellular viability by week 3. The PAF-AF and PA-AF demonstrated significant compression modulus. The presence of collagen type I were detected in all the constructs. The histological findings showed extensive AF cellular distribution. Significant quantitative sGAG was detected for the PAF-AF constructs but with no significant differences on the DNA content. Overall, the PAF-AF constructs support the cellular proliferation of the AF Society for Biomaterials and Artificial Organs - India 2020-07-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82477/19/82477_In%20vitro%20evaluation%20of%20poly_Scopus.pdf application/pdf en http://irep.iium.edu.my/82477/25/82477_In%20vitro%20evaluation%20of%20poly.pdf Mohamad, Mohd Yusof and Mohamed Amin, Muhammad Azri Ifwat and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah (2020) In vitro evaluation of poly (lactic acid-co-glycolic acid)/ atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration. Trends in Biomaterials and Artificial Organs, 34 (3). pp. 118-123. ISSN 0971-1198 https://www.biomaterials.org.in/tibao/index.php/tibao/article/view/389 |
| spellingShingle | R Medicine (General) Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Zulkifly, Ahmad Hafiz Sha'ban, Munirah In vitro evaluation of poly (lactic acid-co-glycolic acid)/ atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration |
| title | In vitro evaluation of poly (lactic acid-co-glycolic acid)/
atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration |
| title_full | In vitro evaluation of poly (lactic acid-co-glycolic acid)/
atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration |
| title_fullStr | In vitro evaluation of poly (lactic acid-co-glycolic acid)/
atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration |
| title_full_unstemmed | In vitro evaluation of poly (lactic acid-co-glycolic acid)/
atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration |
| title_short | In vitro evaluation of poly (lactic acid-co-glycolic acid)/
atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration |
| title_sort | in vitro evaluation of poly (lactic acid-co-glycolic acid)/
atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration |
| topic | R Medicine (General) |
| url | http://irep.iium.edu.my/82477/ http://irep.iium.edu.my/82477/ http://irep.iium.edu.my/82477/19/82477_In%20vitro%20evaluation%20of%20poly_Scopus.pdf http://irep.iium.edu.my/82477/25/82477_In%20vitro%20evaluation%20of%20poly.pdf |