Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides
Considering the significant evidential values of fingerprints in underwater criminal investigations and the need to visualise them using a user- and environmentally-friendly reagent, development of a novel, rapid and relatively greener nanobio-based reagent (NBR) is deemed beneficial. Lipase from th...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
Elsevier Ltd
2020
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| Online Access: | http://irep.iium.edu.my/82448/ http://irep.iium.edu.my/82448/1/82448_Characterisation%20and%20computational%20analysis%20of%20a%20novel_ft.pdf http://irep.iium.edu.my/82448/2/82448_Characterisation%20and%20computational%20analysis%20of%20a%20novel_scopus.pdf http://irep.iium.edu.my/82448/3/82448_Characterisation%20and%20computational%20analysis%20of%20a%20novel_wos.pdf |
| _version_ | 1848789299102744576 |
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| author | Azman, Aida Rasyidah Mahat, Naji Arafat Abdul Wahab, Roswanira Ahmad, Wan Azlina Mohamed Huri, Mohamad Afiq Abdul Hamid, Azzmer Azzar Adamu, Aliyu Mat Saat, Geshina Ayu |
| author_facet | Azman, Aida Rasyidah Mahat, Naji Arafat Abdul Wahab, Roswanira Ahmad, Wan Azlina Mohamed Huri, Mohamad Afiq Abdul Hamid, Azzmer Azzar Adamu, Aliyu Mat Saat, Geshina Ayu |
| author_sort | Azman, Aida Rasyidah |
| building | IIUM Repository |
| collection | Online Access |
| description | Considering the significant evidential values of fingerprints in underwater criminal investigations and the need to visualise them using a user- and environmentally-friendly reagent, development of a novel, rapid and relatively greener nanobio-based reagent (NBR) is deemed beneficial. Lipase from the commercial Candida rugosa immobilised onto acid-functionalised multi-walled carbon nanotubes (NBR) was used as the safer and cheap lipid-sensing reagent to visualise groomed whole/split fingerprints on non-porous objects immersed in stagnant tap water for up to 30 days under a laboratory-controlled setting. Attenuated Total Reflectance – Fourier Transform Spectrometry, Field Emission Scanning Electron Microscopy and bioinformatics (molecular docking and molecular dynamics simulations) were employed to characterise and confirm the attachment of NBR onto the lipid constituents of wet fingerprints. Chromatographic results further confirmed the presence of n-hexadecanoic and octadecanoic acids on fingerprints up to 30 days of immersion. Thus, NBR may potentially be useful as the future state-of-the-art fingerprint visualisation technology. |
| first_indexed | 2025-11-14T17:54:30Z |
| format | Article |
| id | iium-82448 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:54:30Z |
| publishDate | 2020 |
| publisher | Elsevier Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-824482020-09-14T06:43:47Z http://irep.iium.edu.my/82448/ Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides Azman, Aida Rasyidah Mahat, Naji Arafat Abdul Wahab, Roswanira Ahmad, Wan Azlina Mohamed Huri, Mohamad Afiq Abdul Hamid, Azzmer Azzar Adamu, Aliyu Mat Saat, Geshina Ayu Q Science (General) RA Public aspects of medicine Considering the significant evidential values of fingerprints in underwater criminal investigations and the need to visualise them using a user- and environmentally-friendly reagent, development of a novel, rapid and relatively greener nanobio-based reagent (NBR) is deemed beneficial. Lipase from the commercial Candida rugosa immobilised onto acid-functionalised multi-walled carbon nanotubes (NBR) was used as the safer and cheap lipid-sensing reagent to visualise groomed whole/split fingerprints on non-porous objects immersed in stagnant tap water for up to 30 days under a laboratory-controlled setting. Attenuated Total Reflectance – Fourier Transform Spectrometry, Field Emission Scanning Electron Microscopy and bioinformatics (molecular docking and molecular dynamics simulations) were employed to characterise and confirm the attachment of NBR onto the lipid constituents of wet fingerprints. Chromatographic results further confirmed the presence of n-hexadecanoic and octadecanoic acids on fingerprints up to 30 days of immersion. Thus, NBR may potentially be useful as the future state-of-the-art fingerprint visualisation technology. Elsevier Ltd 2020-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82448/1/82448_Characterisation%20and%20computational%20analysis%20of%20a%20novel_ft.pdf application/pdf en http://irep.iium.edu.my/82448/2/82448_Characterisation%20and%20computational%20analysis%20of%20a%20novel_scopus.pdf application/pdf en http://irep.iium.edu.my/82448/3/82448_Characterisation%20and%20computational%20analysis%20of%20a%20novel_wos.pdf Azman, Aida Rasyidah and Mahat, Naji Arafat and Abdul Wahab, Roswanira and Ahmad, Wan Azlina and Mohamed Huri, Mohamad Afiq and Abdul Hamid, Azzmer Azzar and Adamu, Aliyu and Mat Saat, Geshina Ayu (2020) Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides. Process Biochemistry, 96. pp. 102-112. ISSN 1359-5113 E-ISSN 1873-3298 https://www.sciencedirect.com/science/article/abs/pii/S1359511320302154 10.1016/j.procbio.2020.05.033 |
| spellingShingle | Q Science (General) RA Public aspects of medicine Azman, Aida Rasyidah Mahat, Naji Arafat Abdul Wahab, Roswanira Ahmad, Wan Azlina Mohamed Huri, Mohamad Afiq Abdul Hamid, Azzmer Azzar Adamu, Aliyu Mat Saat, Geshina Ayu Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides |
| title | Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides |
| title_full | Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides |
| title_fullStr | Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides |
| title_full_unstemmed | Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides |
| title_short | Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides |
| title_sort | characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides |
| topic | Q Science (General) RA Public aspects of medicine |
| url | http://irep.iium.edu.my/82448/ http://irep.iium.edu.my/82448/ http://irep.iium.edu.my/82448/ http://irep.iium.edu.my/82448/1/82448_Characterisation%20and%20computational%20analysis%20of%20a%20novel_ft.pdf http://irep.iium.edu.my/82448/2/82448_Characterisation%20and%20computational%20analysis%20of%20a%20novel_scopus.pdf http://irep.iium.edu.my/82448/3/82448_Characterisation%20and%20computational%20analysis%20of%20a%20novel_wos.pdf |