GC–MS metabolomics revealed protocatechuic acid as a cytotoxic and apoptosis-inducing compound from black rice brans
GC–MS metabolomics was used to discriminate the phytochemicals profile of Indonesian white, red, and black rice brans, and Japanese white rice brans. This technique was used for the first time to identify compounds in rice brans having cytotoxic activity against WiDr colon cancer cells. Orthogonal P...
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The Korean Society of Food Science and Technology
2020
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| Online Access: | http://irep.iium.edu.my/82261/ http://irep.iium.edu.my/82261/15/82261_GC%E2%80%93MS%20metabolomics%20revealed.pdf http://irep.iium.edu.my/82261/2/82261_GC%E2%80%93MS%20metabolomics%20revealed_SCOPUS.pdf http://irep.iium.edu.my/82261/3/82261_GC%E2%80%93MS%20metabolomics%20revealed_WOS.pdf |
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| author | Yuliana, Nancy Dewi Tuarita, Mirna Zena Khatib, Alfi Laila, Farida Sukarno, Sukarno |
| author_facet | Yuliana, Nancy Dewi Tuarita, Mirna Zena Khatib, Alfi Laila, Farida Sukarno, Sukarno |
| author_sort | Yuliana, Nancy Dewi |
| building | IIUM Repository |
| collection | Online Access |
| description | GC–MS metabolomics was used to discriminate the phytochemicals profile of Indonesian white, red, and black rice brans, and Japanese white rice brans. This technique was used for the first time to identify compounds in rice brans having cytotoxic activity against WiDr colon cancer cells. Orthogonal Projection to the Latent Structure (OPLS) analysis showed that protocatechuic acid (PA) was a discriminating factor found in black rice brans which strongly correlated with its cytotoxicity (IC50 8.53 ± 0.26 µM). Real time-PCR data demonstrated that PA cytotoxicity at different concentrations (1, 5, 10, 25 and 50 µg/mL) was mediated through different pathways. Bcl-2 expression was downregulated at all tested concentrations indicating apoptosis stimulation. At 1–10 ppm concentration, PA activated both intrinsic and extrinsic apoptosis pathways since the expression of p53, Bax, caspase-8, and caspase-9 were upregulated. At a higher dose (25 and 50 µg/mL), PA possibly involved in pyroptosis-mediated pro-inflammatory cell death by upregulating the expression of caspase-1 and caspase-7. |
| first_indexed | 2025-11-14T17:54:02Z |
| format | Article |
| id | iium-82261 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:54:02Z |
| publishDate | 2020 |
| publisher | The Korean Society of Food Science and Technology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-822612020-08-18T07:11:54Z http://irep.iium.edu.my/82261/ GC–MS metabolomics revealed protocatechuic acid as a cytotoxic and apoptosis-inducing compound from black rice brans Yuliana, Nancy Dewi Tuarita, Mirna Zena Khatib, Alfi Laila, Farida Sukarno, Sukarno Q Science (General) QK Botany GC–MS metabolomics was used to discriminate the phytochemicals profile of Indonesian white, red, and black rice brans, and Japanese white rice brans. This technique was used for the first time to identify compounds in rice brans having cytotoxic activity against WiDr colon cancer cells. Orthogonal Projection to the Latent Structure (OPLS) analysis showed that protocatechuic acid (PA) was a discriminating factor found in black rice brans which strongly correlated with its cytotoxicity (IC50 8.53 ± 0.26 µM). Real time-PCR data demonstrated that PA cytotoxicity at different concentrations (1, 5, 10, 25 and 50 µg/mL) was mediated through different pathways. Bcl-2 expression was downregulated at all tested concentrations indicating apoptosis stimulation. At 1–10 ppm concentration, PA activated both intrinsic and extrinsic apoptosis pathways since the expression of p53, Bax, caspase-8, and caspase-9 were upregulated. At a higher dose (25 and 50 µg/mL), PA possibly involved in pyroptosis-mediated pro-inflammatory cell death by upregulating the expression of caspase-1 and caspase-7. The Korean Society of Food Science and Technology 2020-06-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82261/15/82261_GC%E2%80%93MS%20metabolomics%20revealed.pdf application/pdf en http://irep.iium.edu.my/82261/2/82261_GC%E2%80%93MS%20metabolomics%20revealed_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/82261/3/82261_GC%E2%80%93MS%20metabolomics%20revealed_WOS.pdf Yuliana, Nancy Dewi and Tuarita, Mirna Zena and Khatib, Alfi and Laila, Farida and Sukarno, Sukarno (2020) GC–MS metabolomics revealed protocatechuic acid as a cytotoxic and apoptosis-inducing compound from black rice brans. Food Science and Biotechnology, 29 (6). pp. 825-835. ISSN 1226-7708 E-ISSN 2092-6456 https://link.springer.com/article/10.1007/s10068-019-00725-2 10.1007/s10068-019-00725-2 |
| spellingShingle | Q Science (General) QK Botany Yuliana, Nancy Dewi Tuarita, Mirna Zena Khatib, Alfi Laila, Farida Sukarno, Sukarno GC–MS metabolomics revealed protocatechuic acid as a cytotoxic and apoptosis-inducing compound from black rice brans |
| title | GC–MS metabolomics revealed protocatechuic acid as a cytotoxic and apoptosis-inducing compound from black rice brans |
| title_full | GC–MS metabolomics revealed protocatechuic acid as a cytotoxic and apoptosis-inducing compound from black rice brans |
| title_fullStr | GC–MS metabolomics revealed protocatechuic acid as a cytotoxic and apoptosis-inducing compound from black rice brans |
| title_full_unstemmed | GC–MS metabolomics revealed protocatechuic acid as a cytotoxic and apoptosis-inducing compound from black rice brans |
| title_short | GC–MS metabolomics revealed protocatechuic acid as a cytotoxic and apoptosis-inducing compound from black rice brans |
| title_sort | gc–ms metabolomics revealed protocatechuic acid as a cytotoxic and apoptosis-inducing compound from black rice brans |
| topic | Q Science (General) QK Botany |
| url | http://irep.iium.edu.my/82261/ http://irep.iium.edu.my/82261/ http://irep.iium.edu.my/82261/ http://irep.iium.edu.my/82261/15/82261_GC%E2%80%93MS%20metabolomics%20revealed.pdf http://irep.iium.edu.my/82261/2/82261_GC%E2%80%93MS%20metabolomics%20revealed_SCOPUS.pdf http://irep.iium.edu.my/82261/3/82261_GC%E2%80%93MS%20metabolomics%20revealed_WOS.pdf |