MEMS biosensor for monitoring water toxicity based on quartz crystal microbalance

This paper presents the use of a commercial quartz crystal microbalance (QCM) to investigate live-cell activity in water-based toxic solutions. The QCM used in this research has a resonant frequency of 10 MHz and consists of an AT-cut quartz crystal with gold electrodes on both sides. This QCM was t...

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Main Authors: Kun-Lin, Lee, Ng, Simon, Li, Fang, Nordin, Anis Nurashikin, Voiculescu, Ioana
Format: Article
Language:English
English
English
Published: American Institute of Physics Inc. 2020
Subjects:
Online Access:http://irep.iium.edu.my/82225/
http://irep.iium.edu.my/82225/19/82225_MEMS%20biosensor%20for%20monitoring%20water.pdf
http://irep.iium.edu.my/82225/2/82225_MEMS%20biosensor%20for%20monitoring_SCOPUS.pdf
http://irep.iium.edu.my/82225/3/82225_MEMS%20biosensor%20for%20monitoring_WOS.pdf
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author Kun-Lin, Lee
Ng, Simon
Li, Fang
Nordin, Anis Nurashikin
Voiculescu, Ioana
author_facet Kun-Lin, Lee
Ng, Simon
Li, Fang
Nordin, Anis Nurashikin
Voiculescu, Ioana
author_sort Kun-Lin, Lee
building IIUM Repository
collection Online Access
description This paper presents the use of a commercial quartz crystal microbalance (QCM) to investigate live-cell activity in water-based toxic solutions. The QCM used in this research has a resonant frequency of 10 MHz and consists of an AT-cut quartz crystal with gold electrodes on both sides. This QCM was transformed into a functional biosensor by integrating with polydimethylsiloxane culturing chambers. Rainbow trout gill epithelial cells were cultured on the resonators as a sensorial layer. The fluctuation of the resonant frequency, due to the change of cell morphology and adhesion, is an indicator of water toxicity. The shift in the resonant frequency provides information about the viability of the cells after exposure to toxicants. The toxicity result shows distinct responses after exposing cells to 0.526 μM of pentachlorophenol (PCP) solution, which is the Military Exposure Guidelines concentration. This research demonstrated that the QCM is sensitive to a low concentration of PCP and no further modification of the QCM surface was required.
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format Article
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institution International Islamic University Malaysia
institution_category Local University
language English
English
English
last_indexed 2025-11-14T17:53:56Z
publishDate 2020
publisher American Institute of Physics Inc.
recordtype eprints
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spelling iium-822252020-08-24T09:07:04Z http://irep.iium.edu.my/82225/ MEMS biosensor for monitoring water toxicity based on quartz crystal microbalance Kun-Lin, Lee Ng, Simon Li, Fang Nordin, Anis Nurashikin Voiculescu, Ioana TK Electrical engineering. Electronics Nuclear engineering TK4001 Applications of electric power This paper presents the use of a commercial quartz crystal microbalance (QCM) to investigate live-cell activity in water-based toxic solutions. The QCM used in this research has a resonant frequency of 10 MHz and consists of an AT-cut quartz crystal with gold electrodes on both sides. This QCM was transformed into a functional biosensor by integrating with polydimethylsiloxane culturing chambers. Rainbow trout gill epithelial cells were cultured on the resonators as a sensorial layer. The fluctuation of the resonant frequency, due to the change of cell morphology and adhesion, is an indicator of water toxicity. The shift in the resonant frequency provides information about the viability of the cells after exposure to toxicants. The toxicity result shows distinct responses after exposing cells to 0.526 μM of pentachlorophenol (PCP) solution, which is the Military Exposure Guidelines concentration. This research demonstrated that the QCM is sensitive to a low concentration of PCP and no further modification of the QCM surface was required. American Institute of Physics Inc. 2020-03-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82225/19/82225_MEMS%20biosensor%20for%20monitoring%20water.pdf application/pdf en http://irep.iium.edu.my/82225/2/82225_MEMS%20biosensor%20for%20monitoring_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/82225/3/82225_MEMS%20biosensor%20for%20monitoring_WOS.pdf Kun-Lin, Lee and Ng, Simon and Li, Fang and Nordin, Anis Nurashikin and Voiculescu, Ioana (2020) MEMS biosensor for monitoring water toxicity based on quartz crystal microbalance. Biointerphases, 15 (2). pp. 1-3. ISSN 1934-8630 E-ISSN 1559-4106 https://avs.scitation.org/doi/10.1116/1.5142722 10.1116/1.5142722
spellingShingle TK Electrical engineering. Electronics Nuclear engineering
TK4001 Applications of electric power
Kun-Lin, Lee
Ng, Simon
Li, Fang
Nordin, Anis Nurashikin
Voiculescu, Ioana
MEMS biosensor for monitoring water toxicity based on quartz crystal microbalance
title MEMS biosensor for monitoring water toxicity based on quartz crystal microbalance
title_full MEMS biosensor for monitoring water toxicity based on quartz crystal microbalance
title_fullStr MEMS biosensor for monitoring water toxicity based on quartz crystal microbalance
title_full_unstemmed MEMS biosensor for monitoring water toxicity based on quartz crystal microbalance
title_short MEMS biosensor for monitoring water toxicity based on quartz crystal microbalance
title_sort mems biosensor for monitoring water toxicity based on quartz crystal microbalance
topic TK Electrical engineering. Electronics Nuclear engineering
TK4001 Applications of electric power
url http://irep.iium.edu.my/82225/
http://irep.iium.edu.my/82225/
http://irep.iium.edu.my/82225/
http://irep.iium.edu.my/82225/19/82225_MEMS%20biosensor%20for%20monitoring%20water.pdf
http://irep.iium.edu.my/82225/2/82225_MEMS%20biosensor%20for%20monitoring_SCOPUS.pdf
http://irep.iium.edu.my/82225/3/82225_MEMS%20biosensor%20for%20monitoring_WOS.pdf