Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats
This study aimed to identify the bioactive compounds of the ethyl acetate extract of Aspergillus niger SH2-EGY using GC-MS and to evaluate their protective role against aflatoxin B1 (AFB1)-induced oxidative stress, genotoxicity and cytotoxicity in rats. Six groups of male Sprague-Dawley rats were tr...
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Elsevier Ltd
2020
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| Online Access: | http://irep.iium.edu.my/82209/ http://irep.iium.edu.my/82209/2/82209_Bioactive%20compounds%20from%20Aspergillus%20niger%20extract_FULL%20TEXT.pdf http://irep.iium.edu.my/82209/1/82209_Bioactive%20compounds%20from%20Aspergillus%20niger%20extract_SCOPUS.pdf |
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| author | Abdel-Wahhab, Mosaad Attia El-Nekeety, Aziza Abdel Salam Hathout, Amal S. Salman, Asmaa S. Abdel-Aziem, Sekena H. Sabry, Bassem A. Hassan, Nabila Salah Abdel-Aziz, Mohammed S. Aly, Soher E. Jaswir, Irwandi |
| author_facet | Abdel-Wahhab, Mosaad Attia El-Nekeety, Aziza Abdel Salam Hathout, Amal S. Salman, Asmaa S. Abdel-Aziem, Sekena H. Sabry, Bassem A. Hassan, Nabila Salah Abdel-Aziz, Mohammed S. Aly, Soher E. Jaswir, Irwandi |
| author_sort | Abdel-Wahhab, Mosaad Attia |
| building | IIUM Repository |
| collection | Online Access |
| description | This study aimed to identify the bioactive compounds of the ethyl acetate extract of Aspergillus niger SH2-EGY using GC-MS and to evaluate their protective role against aflatoxin B1 (AFB1)-induced oxidative stress, genotoxicity and cytotoxicity in rats. Six groups of male Sprague-Dawley rats were treated orally for 4 weeks included the control group, AFB1-treated group (80 μg/kg b.w); fungal extract (FE)-treated groups at low (140) or high dose (280) mg/kg b.w and the groups treated with AFB1 plus FE at the two tested doses. The GC-MS analysis identified 26 compounds. The major compounds found were 1,2,3,4,6-Penta-trimethylsilyl Glucopyranose, Fmoc-L-3-(2-Naphthyl)-alanine, D-(-)-Fructopyranose, pentakis (trimethylsilyl) ether, bis (2-ethylhexyl) phthalate, trimethylsilyl ether-glucitol, and octadecanamide, N-(2- methylpropyl)-N-nitroso. The in vivo results showed that AFB1 significantly increased serum ALT, AST, creatinine, uric acid, urea, cholesterol, triglycerides, LDL, carcinoembryonic antigen, alpha-fetoprotein, interleukin-6, Malondialdehyde, nitric oxide, Bax, caspase-3 and P53 mRNA expression, chromosomal aberrations and DNA fragmentation. It decreased serum TP, albumin, HDL, Bcl-2 mRNA expression, hepatic and renal TAC, SOD and GPx content and induced histological changes in the liver and kidney. FE prevented these disturbances in a dosage-dependent manner. It could be concluded that A. niger SH2-EGY extract is safe a promising agent for pharmaceutical and food industries. |
| first_indexed | 2025-11-14T17:53:52Z |
| format | Article |
| id | iium-82209 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:53:52Z |
| publishDate | 2020 |
| publisher | Elsevier Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-822092020-10-06T07:30:06Z http://irep.iium.edu.my/82209/ Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats Abdel-Wahhab, Mosaad Attia El-Nekeety, Aziza Abdel Salam Hathout, Amal S. Salman, Asmaa S. Abdel-Aziem, Sekena H. Sabry, Bassem A. Hassan, Nabila Salah Abdel-Aziz, Mohammed S. Aly, Soher E. Jaswir, Irwandi QH301 Biology TP Chemical technology TP368 Food processing and manufacture This study aimed to identify the bioactive compounds of the ethyl acetate extract of Aspergillus niger SH2-EGY using GC-MS and to evaluate their protective role against aflatoxin B1 (AFB1)-induced oxidative stress, genotoxicity and cytotoxicity in rats. Six groups of male Sprague-Dawley rats were treated orally for 4 weeks included the control group, AFB1-treated group (80 μg/kg b.w); fungal extract (FE)-treated groups at low (140) or high dose (280) mg/kg b.w and the groups treated with AFB1 plus FE at the two tested doses. The GC-MS analysis identified 26 compounds. The major compounds found were 1,2,3,4,6-Penta-trimethylsilyl Glucopyranose, Fmoc-L-3-(2-Naphthyl)-alanine, D-(-)-Fructopyranose, pentakis (trimethylsilyl) ether, bis (2-ethylhexyl) phthalate, trimethylsilyl ether-glucitol, and octadecanamide, N-(2- methylpropyl)-N-nitroso. The in vivo results showed that AFB1 significantly increased serum ALT, AST, creatinine, uric acid, urea, cholesterol, triglycerides, LDL, carcinoembryonic antigen, alpha-fetoprotein, interleukin-6, Malondialdehyde, nitric oxide, Bax, caspase-3 and P53 mRNA expression, chromosomal aberrations and DNA fragmentation. It decreased serum TP, albumin, HDL, Bcl-2 mRNA expression, hepatic and renal TAC, SOD and GPx content and induced histological changes in the liver and kidney. FE prevented these disturbances in a dosage-dependent manner. It could be concluded that A. niger SH2-EGY extract is safe a promising agent for pharmaceutical and food industries. Elsevier Ltd 2020-07-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82209/2/82209_Bioactive%20compounds%20from%20Aspergillus%20niger%20extract_FULL%20TEXT.pdf application/pdf en http://irep.iium.edu.my/82209/1/82209_Bioactive%20compounds%20from%20Aspergillus%20niger%20extract_SCOPUS.pdf Abdel-Wahhab, Mosaad Attia and El-Nekeety, Aziza Abdel Salam and Hathout, Amal S. and Salman, Asmaa S. and Abdel-Aziem, Sekena H. and Sabry, Bassem A. and Hassan, Nabila Salah and Abdel-Aziz, Mohammed S. and Aly, Soher E. and Jaswir, Irwandi (2020) Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats. Toxicon, 181. pp. 57-68. ISSN 0041-0101 E-ISSN 1879-3150 https://www.sciencedirect.com/science/article/abs/pii/S004101012030221X 10.1016/j.toxicon.2020.04.103 |
| spellingShingle | QH301 Biology TP Chemical technology TP368 Food processing and manufacture Abdel-Wahhab, Mosaad Attia El-Nekeety, Aziza Abdel Salam Hathout, Amal S. Salman, Asmaa S. Abdel-Aziem, Sekena H. Sabry, Bassem A. Hassan, Nabila Salah Abdel-Aziz, Mohammed S. Aly, Soher E. Jaswir, Irwandi Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats |
| title | Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats |
| title_full | Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats |
| title_fullStr | Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats |
| title_full_unstemmed | Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats |
| title_short | Bioactive compounds from Aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin B1-treated rats |
| title_sort | bioactive compounds from aspergillus niger extract enhance the antioxidant activity and prevent the genotoxicity in aflatoxin b1-treated rats |
| topic | QH301 Biology TP Chemical technology TP368 Food processing and manufacture |
| url | http://irep.iium.edu.my/82209/ http://irep.iium.edu.my/82209/ http://irep.iium.edu.my/82209/ http://irep.iium.edu.my/82209/2/82209_Bioactive%20compounds%20from%20Aspergillus%20niger%20extract_FULL%20TEXT.pdf http://irep.iium.edu.my/82209/1/82209_Bioactive%20compounds%20from%20Aspergillus%20niger%20extract_SCOPUS.pdf |