Production of mitotic gynogenesis of Masu salmon in Japan using pressure shock
Chromosome manipulation techniques have been adopted in aquaculture to improve breeding selection and performance. One of the approaches in chromosome manipulation is gynogenesis. This study aims to identify the time duration of pressure shock applied to the eggs after fertilisation. UV irradiated s...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kulliyyah of Allied Health Sciences, International Islamic University Malaysia.
2019
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/82159/ http://irep.iium.edu.my/82159/1/2019_%20324-Article%20Text-1827-1-10-20200107.pdf |
| _version_ | 1848789247697354752 |
|---|---|
| author | Yusof, Fitri Nakajima, Masamichi Kawada, Gyo |
| author_facet | Yusof, Fitri Nakajima, Masamichi Kawada, Gyo |
| author_sort | Yusof, Fitri |
| building | IIUM Repository |
| collection | Online Access |
| description | Chromosome manipulation techniques have been adopted in aquaculture to improve breeding selection and performance. One of the approaches in chromosome manipulation is gynogenesis. This study aims to identify the time duration of pressure shock applied to the eggs after fertilisation. UV irradiated sperm was used to fertilised eggs of masu salmon (Oncorhynchus masou). Pressure shock of 650 kg/cm2 for 6 minutes 30 seconds was given on 56, 58, 60, 62 and 64 minutes after fertilisation (AF). Intact Control (IC) and Gynogenetic Control (GC) were produced by fertilising eggs with fertile sperm and inducing retention of second polar body (haploid) respectively. Ploidy level was verified using microsatellite markers while the percentage of hatching rate, swim-up rate and normal fries were analysed using One Way ANOVA. Results showed 100% success in producing diploid mitotic gynogen for all different time duration. Yet, hatching rate, swim-up rate and the normal rate of all treatment significantly low in comparison to normal control (IC). This study suggests the effectiveness of pressure shock in producing diploid mitotic gynogen and its verification using microsatellite markers. However, further study is needed to improve the success rate. |
| first_indexed | 2025-11-14T17:53:41Z |
| format | Article |
| id | iium-82159 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:53:41Z |
| publishDate | 2019 |
| publisher | Kulliyyah of Allied Health Sciences, International Islamic University Malaysia. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-821592020-09-15T01:19:31Z http://irep.iium.edu.my/82159/ Production of mitotic gynogenesis of Masu salmon in Japan using pressure shock Yusof, Fitri Nakajima, Masamichi Kawada, Gyo QH301 Biology QH426 Genetics QH471 Reproduction SH151 Aquaculture - Fish Culture Chromosome manipulation techniques have been adopted in aquaculture to improve breeding selection and performance. One of the approaches in chromosome manipulation is gynogenesis. This study aims to identify the time duration of pressure shock applied to the eggs after fertilisation. UV irradiated sperm was used to fertilised eggs of masu salmon (Oncorhynchus masou). Pressure shock of 650 kg/cm2 for 6 minutes 30 seconds was given on 56, 58, 60, 62 and 64 minutes after fertilisation (AF). Intact Control (IC) and Gynogenetic Control (GC) were produced by fertilising eggs with fertile sperm and inducing retention of second polar body (haploid) respectively. Ploidy level was verified using microsatellite markers while the percentage of hatching rate, swim-up rate and normal fries were analysed using One Way ANOVA. Results showed 100% success in producing diploid mitotic gynogen for all different time duration. Yet, hatching rate, swim-up rate and the normal rate of all treatment significantly low in comparison to normal control (IC). This study suggests the effectiveness of pressure shock in producing diploid mitotic gynogen and its verification using microsatellite markers. However, further study is needed to improve the success rate. Kulliyyah of Allied Health Sciences, International Islamic University Malaysia. 2019-12-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82159/1/2019_%20324-Article%20Text-1827-1-10-20200107.pdf Yusof, Fitri and Nakajima, Masamichi and Kawada, Gyo (2019) Production of mitotic gynogenesis of Masu salmon in Japan using pressure shock. International Journal of Allied Health Sciences, 3 (3 Special Issue: Research Towards Sustainable Development Goals). p. 764. E-ISSN 2600-8491 https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/324/316 |
| spellingShingle | QH301 Biology QH426 Genetics QH471 Reproduction SH151 Aquaculture - Fish Culture Yusof, Fitri Nakajima, Masamichi Kawada, Gyo Production of mitotic gynogenesis of Masu salmon in Japan using pressure shock |
| title | Production of mitotic gynogenesis of Masu salmon in Japan using pressure shock |
| title_full | Production of mitotic gynogenesis of Masu salmon in Japan using pressure shock |
| title_fullStr | Production of mitotic gynogenesis of Masu salmon in Japan using pressure shock |
| title_full_unstemmed | Production of mitotic gynogenesis of Masu salmon in Japan using pressure shock |
| title_short | Production of mitotic gynogenesis of Masu salmon in Japan using pressure shock |
| title_sort | production of mitotic gynogenesis of masu salmon in japan using pressure shock |
| topic | QH301 Biology QH426 Genetics QH471 Reproduction SH151 Aquaculture - Fish Culture |
| url | http://irep.iium.edu.my/82159/ http://irep.iium.edu.my/82159/ http://irep.iium.edu.my/82159/1/2019_%20324-Article%20Text-1827-1-10-20200107.pdf |