Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits
Oxidative stress and reactive oxygen species (ROS) constitute a major pathogenic mechanism for the development of atherosclerosis. In the present work, the antioxidant potential of Trihoney was investigated in hypercholesterolaemic rabbits. Thirty-six male New Zealand white (NZW) rabbits were groupe...
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| Format: | Article |
| Language: | English English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2020
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| Online Access: | http://irep.iium.edu.my/82073/ http://irep.iium.edu.my/82073/8/82073%20Trihoney%20reduces%20lipid%20peroxidation.pdf http://irep.iium.edu.my/82073/7/82073%20Trihoney%20reduces%20lipid%20peroxidation%20SCOPUS.pdf |
| _version_ | 1848789231784165376 |
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| author | Hamad Alfarisi, Hamad Abdulsalam Ibrahim, Muhammad Hamad Mohamed, Zenab Hamdan, Asmah Hanim Che Mohamad, Che Anuar |
| author_facet | Hamad Alfarisi, Hamad Abdulsalam Ibrahim, Muhammad Hamad Mohamed, Zenab Hamdan, Asmah Hanim Che Mohamad, Che Anuar |
| author_sort | Hamad Alfarisi, Hamad Abdulsalam |
| building | IIUM Repository |
| collection | Online Access |
| description | Oxidative stress and reactive oxygen species (ROS) constitute a major pathogenic mechanism for the development of atherosclerosis. In the present work, the antioxidant potential of Trihoney was investigated in hypercholesterolaemic rabbits. Thirty-six male New Zealand white (NZW) rabbits were grouped into: normal diet (C), normal diet with 0.6 g/kg/day of Trihoney (C+H), 1% cholesterol diet (HCD), 1% cholesterol diet with 0.3 g/kg/day of Trihoney (HCD+H1), 1% cholesterol diet with 0.6 g/kg/day of Trihoney (HCD+H2), and 1% cholesterol diet with 2 mg/kg/day of atorvastatin (HCD+At.). Animals were sacrificed following 12 weeks of treatment, and their serum was analysed for oxidised-low density lipoprotein (Ox-LDL). Serum and aortic tissue homogenate were assayed for superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA). Hypercholesterolemia caused a significant (p < 0.05) elevation in serum Ox-LDL and a significant (p < 0.05) reduction of antioxidant enzyme activities in serum of the HCD group. Trihoney induced a significant (p < 0.05) increase in antioxidant enzyme activities in serum as compared to the HCD group. The high cholesterol diet suppressed both antioxidant enzymes in aortic homogenate. Trihoney significantly (p < 0.05) enhanced both antioxidant enzymes in aortic homogenate. Hypercholesterolemia induced a significant (p < 0.05) elevation of serum lipid peroxidation in the HCD group. Trihoney caused a significant (p < 0.05) reduction of lipid peroxidation in aortic homogenate. These results demonstrated that Trihoney has the potential to ameliorate oxidative stress systemically, as well as locally in the atherosclerotic aorta. |
| first_indexed | 2025-11-14T17:53:26Z |
| format | Article |
| id | iium-82073 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:53:26Z |
| publishDate | 2020 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-820732023-11-21T01:24:38Z http://irep.iium.edu.my/82073/ Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits Hamad Alfarisi, Hamad Abdulsalam Ibrahim, Muhammad Hamad Mohamed, Zenab Hamdan, Asmah Hanim Che Mohamad, Che Anuar Q Science (General) Oxidative stress and reactive oxygen species (ROS) constitute a major pathogenic mechanism for the development of atherosclerosis. In the present work, the antioxidant potential of Trihoney was investigated in hypercholesterolaemic rabbits. Thirty-six male New Zealand white (NZW) rabbits were grouped into: normal diet (C), normal diet with 0.6 g/kg/day of Trihoney (C+H), 1% cholesterol diet (HCD), 1% cholesterol diet with 0.3 g/kg/day of Trihoney (HCD+H1), 1% cholesterol diet with 0.6 g/kg/day of Trihoney (HCD+H2), and 1% cholesterol diet with 2 mg/kg/day of atorvastatin (HCD+At.). Animals were sacrificed following 12 weeks of treatment, and their serum was analysed for oxidised-low density lipoprotein (Ox-LDL). Serum and aortic tissue homogenate were assayed for superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA). Hypercholesterolemia caused a significant (p < 0.05) elevation in serum Ox-LDL and a significant (p < 0.05) reduction of antioxidant enzyme activities in serum of the HCD group. Trihoney induced a significant (p < 0.05) increase in antioxidant enzyme activities in serum as compared to the HCD group. The high cholesterol diet suppressed both antioxidant enzymes in aortic homogenate. Trihoney significantly (p < 0.05) enhanced both antioxidant enzymes in aortic homogenate. Hypercholesterolemia induced a significant (p < 0.05) elevation of serum lipid peroxidation in the HCD group. Trihoney caused a significant (p < 0.05) reduction of lipid peroxidation in aortic homogenate. These results demonstrated that Trihoney has the potential to ameliorate oxidative stress systemically, as well as locally in the atherosclerotic aorta. Faculty of Food Science and Technology, Universiti Putra Malaysia 2020-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/82073/8/82073%20Trihoney%20reduces%20lipid%20peroxidation.pdf application/pdf en http://irep.iium.edu.my/82073/7/82073%20Trihoney%20reduces%20lipid%20peroxidation%20SCOPUS.pdf Hamad Alfarisi, Hamad Abdulsalam and Ibrahim, Muhammad and Hamad Mohamed, Zenab and Hamdan, Asmah Hanim and Che Mohamad, Che Anuar (2020) Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits. International Food Research Journal, 27 (3). pp. 568-575. E-ISSN 2231-7546 http://www.ifrj.upm.edu.my/27%20(03)%202020/DONE%20-%2018%20-%20IFRJ19853.R1.pdf |
| spellingShingle | Q Science (General) Hamad Alfarisi, Hamad Abdulsalam Ibrahim, Muhammad Hamad Mohamed, Zenab Hamdan, Asmah Hanim Che Mohamad, Che Anuar Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits |
| title | Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits |
| title_full | Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits |
| title_fullStr | Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits |
| title_full_unstemmed | Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits |
| title_short | Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits |
| title_sort | trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/82073/ http://irep.iium.edu.my/82073/ http://irep.iium.edu.my/82073/8/82073%20Trihoney%20reduces%20lipid%20peroxidation.pdf http://irep.iium.edu.my/82073/7/82073%20Trihoney%20reduces%20lipid%20peroxidation%20SCOPUS.pdf |