Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits
Oxidative stress and reactive oxygen species (ROS) constitute a major pathogenic mechanism for the development of atherosclerosis. In the present work, the antioxidant potential of Trihoney was investigated in hypercholesterolaemic rabbits. Thirty-six male New Zealand white (NZW) rabbits were groupe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2020
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/82073/ http://irep.iium.edu.my/82073/8/82073%20Trihoney%20reduces%20lipid%20peroxidation.pdf http://irep.iium.edu.my/82073/7/82073%20Trihoney%20reduces%20lipid%20peroxidation%20SCOPUS.pdf |
| Summary: | Oxidative stress and reactive oxygen species (ROS) constitute a major pathogenic mechanism for the development of atherosclerosis. In the present work, the antioxidant potential of Trihoney was investigated in hypercholesterolaemic rabbits. Thirty-six male New Zealand white (NZW) rabbits were grouped into: normal diet (C), normal diet with 0.6 g/kg/day of Trihoney (C+H), 1% cholesterol diet (HCD), 1% cholesterol diet with 0.3 g/kg/day of Trihoney (HCD+H1), 1% cholesterol diet with 0.6 g/kg/day of Trihoney (HCD+H2), and 1% cholesterol diet with 2 mg/kg/day of atorvastatin (HCD+At.). Animals were sacrificed following 12 weeks of treatment, and their serum was analysed for oxidised-low density lipoprotein (Ox-LDL). Serum and aortic tissue homogenate were assayed for superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA). Hypercholesterolemia caused a significant (p < 0.05) elevation in serum Ox-LDL and a significant (p < 0.05) reduction of antioxidant enzyme activities in serum of the HCD group. Trihoney induced a significant (p < 0.05) increase in antioxidant enzyme activities in serum as compared to the HCD group. The high cholesterol diet suppressed both antioxidant enzymes in aortic homogenate. Trihoney significantly (p < 0.05) enhanced both antioxidant enzymes in aortic homogenate. Hypercholesterolemia induced a significant (p < 0.05) elevation of serum lipid peroxidation in the HCD group. Trihoney caused a significant (p < 0.05) reduction of lipid peroxidation in aortic homogenate. These results demonstrated that Trihoney has the potential to ameliorate oxidative stress systemically, as well as locally in the atherosclerotic aorta. |
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