Polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation
Dendrimers are highly branched synthetic macromolecules that offer promising prospects for utilizing them in broad applications. This study investigates the role of polyamidoamine (PAMAM) dendrimer for lipase-catalyzed reaction in aqueous solution. The hydrolysis of para-nitrophenyl palmitate (pNPP)...
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Elsevier Ltd. All rights
2020
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| Online Access: | http://irep.iium.edu.my/81987/ http://irep.iium.edu.my/81987/1/81987_Polyamidoamine%20dendrimers%20-%20in%20press.pdf http://irep.iium.edu.my/81987/7/81987_Polyamidoamine%20dendrimers_SCOPUS.pdf |
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| author | Elgharbawy, Amal A.M. Syed Putra, Sharifah Shahira Hayyan, Maan Hayyan, Adeeb Basirun, Wan Jefrey Mirghani, Mohamed Elwathig Saeed Wan Nawawi, Wan Mohd Fazli Majrashi, Abdulrahman Abdulaziz Alias, Yatimah Mohd. Salleh, Hamzah Tok, Tugba Taskin |
| author_facet | Elgharbawy, Amal A.M. Syed Putra, Sharifah Shahira Hayyan, Maan Hayyan, Adeeb Basirun, Wan Jefrey Mirghani, Mohamed Elwathig Saeed Wan Nawawi, Wan Mohd Fazli Majrashi, Abdulrahman Abdulaziz Alias, Yatimah Mohd. Salleh, Hamzah Tok, Tugba Taskin |
| author_sort | Elgharbawy, Amal A.M. |
| building | IIUM Repository |
| collection | Online Access |
| description | Dendrimers are highly branched synthetic macromolecules that offer promising prospects for utilizing them in broad applications. This study investigates the role of polyamidoamine (PAMAM) dendrimer for lipase-catalyzed reaction in aqueous solution. The hydrolysis of para-nitrophenyl palmitate (pNPP) by four different lipases was tested with PAMAM dendrimer with different generations (i.e., D0.0, D2.5, D3.0, and D4.0). The results showed that the activity of Rhizopus niveus lipase (RNL) was enhanced up to 375% with PAMAM dendrimer (D0.0). The optimum incubation conditions such as the temperature and coupling time of the lipase with D0.0 were 40 °C and 2 h, respectively. The behavior of lipases at different pH showed that RNL was mostly active at pH 7.0. Most lipases with D0.0 were enhanced at least two-fold compared to the control samples. Kinetic study showed that the RNL affinity was enhanced in the presence of PAMAM. FTIR spectra strongly suggest that hydrogen bonding and electrostatic interactions were the main types of bonding in the RNL and PAMAM system. Additionally, the molecular docking simulation of the system was exerted to evaluate and support the related compound, (i.e., D0.0) as a ligand interacted effectively with RNL based on the experimental activity. The cytotoxicity study revealed mild toxicity of the system which could serve as a potential target in nanomedicine for many applications such as cancer therapeutics. |
| first_indexed | 2025-11-14T17:53:13Z |
| format | Article |
| id | iium-81987 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:53:13Z |
| publishDate | 2020 |
| publisher | Elsevier Ltd. All rights |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-819872020-12-29T08:08:57Z http://irep.iium.edu.my/81987/ Polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation Elgharbawy, Amal A.M. Syed Putra, Sharifah Shahira Hayyan, Maan Hayyan, Adeeb Basirun, Wan Jefrey Mirghani, Mohamed Elwathig Saeed Wan Nawawi, Wan Mohd Fazli Majrashi, Abdulrahman Abdulaziz Alias, Yatimah Mohd. Salleh, Hamzah Tok, Tugba Taskin Q Science (General) T Technology (General) Dendrimers are highly branched synthetic macromolecules that offer promising prospects for utilizing them in broad applications. This study investigates the role of polyamidoamine (PAMAM) dendrimer for lipase-catalyzed reaction in aqueous solution. The hydrolysis of para-nitrophenyl palmitate (pNPP) by four different lipases was tested with PAMAM dendrimer with different generations (i.e., D0.0, D2.5, D3.0, and D4.0). The results showed that the activity of Rhizopus niveus lipase (RNL) was enhanced up to 375% with PAMAM dendrimer (D0.0). The optimum incubation conditions such as the temperature and coupling time of the lipase with D0.0 were 40 °C and 2 h, respectively. The behavior of lipases at different pH showed that RNL was mostly active at pH 7.0. Most lipases with D0.0 were enhanced at least two-fold compared to the control samples. Kinetic study showed that the RNL affinity was enhanced in the presence of PAMAM. FTIR spectra strongly suggest that hydrogen bonding and electrostatic interactions were the main types of bonding in the RNL and PAMAM system. Additionally, the molecular docking simulation of the system was exerted to evaluate and support the related compound, (i.e., D0.0) as a ligand interacted effectively with RNL based on the experimental activity. The cytotoxicity study revealed mild toxicity of the system which could serve as a potential target in nanomedicine for many applications such as cancer therapeutics. Elsevier Ltd. All rights 2020-07-28 Article PeerReviewed application/pdf en http://irep.iium.edu.my/81987/1/81987_Polyamidoamine%20dendrimers%20-%20in%20press.pdf application/pdf en http://irep.iium.edu.my/81987/7/81987_Polyamidoamine%20dendrimers_SCOPUS.pdf Elgharbawy, Amal A.M. and Syed Putra, Sharifah Shahira and Hayyan, Maan and Hayyan, Adeeb and Basirun, Wan Jefrey and Mirghani, Mohamed Elwathig Saeed and Wan Nawawi, Wan Mohd Fazli and Majrashi, Abdulrahman Abdulaziz and Alias, Yatimah and Mohd. Salleh, Hamzah and Tok, Tugba Taskin (2020) Polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation. Materials Today Communications, 26. p. 101492. ISSN 2352-4928 https://www.sciencedirect.com/journal/materials-today-communications https://doi.org/10.1016/j.mtcomm.2020.101492 |
| spellingShingle | Q Science (General) T Technology (General) Elgharbawy, Amal A.M. Syed Putra, Sharifah Shahira Hayyan, Maan Hayyan, Adeeb Basirun, Wan Jefrey Mirghani, Mohamed Elwathig Saeed Wan Nawawi, Wan Mohd Fazli Majrashi, Abdulrahman Abdulaziz Alias, Yatimah Mohd. Salleh, Hamzah Tok, Tugba Taskin Polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation |
| title | Polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation |
| title_full | Polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation |
| title_fullStr | Polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation |
| title_full_unstemmed | Polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation |
| title_short | Polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation |
| title_sort | polyamidoamine dendrimers: favorable polymeric nanomaterials for lipase activation |
| topic | Q Science (General) T Technology (General) |
| url | http://irep.iium.edu.my/81987/ http://irep.iium.edu.my/81987/ http://irep.iium.edu.my/81987/ http://irep.iium.edu.my/81987/1/81987_Polyamidoamine%20dendrimers%20-%20in%20press.pdf http://irep.iium.edu.my/81987/7/81987_Polyamidoamine%20dendrimers_SCOPUS.pdf |