Preliminary study on the effect of Tualang honey and its silver nanoparticles on Kainic acid mediated behavioural changes in male rats
Objectives: Excitotoxicity is a key mechanism in neurodegenerative diseases. Kainic acid (KA) is used to induce experimental excitotoxicity. Therefore, the present study investigates the effect of Tualang honey (TH) and its silver nanoparticles (THSN) on KA mediated behavioural changes in rats....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Sains Malaysia
2020
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/81960/ http://irep.iium.edu.my/81960/1/01MJMS27si2020.pdf |
| _version_ | 1848789213706715136 |
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| author | Hasim, Hidani Zulkifli, Nurdarina Ausi Asari, Mohd Asnizam Muthuraju, Sangu Visweswara Rao, Pasupuleti Visweswara Mustapha, Muzaimi Kuttulebbai Naina Mohamed Salam, Sirajudeen |
| author_facet | Hasim, Hidani Zulkifli, Nurdarina Ausi Asari, Mohd Asnizam Muthuraju, Sangu Visweswara Rao, Pasupuleti Visweswara Mustapha, Muzaimi Kuttulebbai Naina Mohamed Salam, Sirajudeen |
| author_sort | Hasim, Hidani |
| building | IIUM Repository |
| collection | Online Access |
| description | Objectives: Excitotoxicity is a key mechanism in
neurodegenerative diseases. Kainic acid (KA) is used to
induce experimental excitotoxicity. Therefore, the present
study investigates the effect of Tualang honey (TH) and its
silver nanoparticles (THSN) on KA mediated behavioural
changes in rats.
Methods: Adult Sprague-Dawley male rats (n = 18)
were randomised into six groups (n = 3/group): i) Control,
ii) THSN, iii) KA, iv) TH + KA, v) THSN + KA and vi)
Topiramate + KA groups which were pre-treated orally with distilled water, THSN (50 mg/kg body weight), Tualang honey (1.0 g/kg body weight) or Topiramate (40 mg/kg body weight), respectively, five times at 12 h intervals. Saline or KA (15 mg/kg body weight) were injected subcutaneously 30 min after last oral treatment. The latency to onset of the first generalised seizures (FGS) was recorded. Behavioural assessments such as open field test (OFT) to assess the locomotor activity and novel object recognition test (NORT) for learning and memory were performed after 24 h of KA administration.
Results: On KA treatment, FGS was observed. But
there is a trend of amelioration of FGS on TH+KA and
THSN+KA groups when compared to KA group. Pretreatment with TH and THSN improved learning and
memory as evidenced by increased recognition index in
NORT when compared to untreated KA group. Interestingly, OFT results of THSN+KA group improved the locomotor activity when compared to untreated KA group.
Conclusion: These findings suggest that pre-treatment
with TH and THSN provides protective effect on KA mediated behavioural changes in male rats. However, further study required to understand the mechanism of action. |
| first_indexed | 2025-11-14T17:53:09Z |
| format | Article |
| id | iium-81960 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:53:09Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-819602021-01-11T04:47:29Z http://irep.iium.edu.my/81960/ Preliminary study on the effect of Tualang honey and its silver nanoparticles on Kainic acid mediated behavioural changes in male rats Hasim, Hidani Zulkifli, Nurdarina Ausi Asari, Mohd Asnizam Muthuraju, Sangu Visweswara Rao, Pasupuleti Visweswara Mustapha, Muzaimi Kuttulebbai Naina Mohamed Salam, Sirajudeen R Medicine (General) Objectives: Excitotoxicity is a key mechanism in neurodegenerative diseases. Kainic acid (KA) is used to induce experimental excitotoxicity. Therefore, the present study investigates the effect of Tualang honey (TH) and its silver nanoparticles (THSN) on KA mediated behavioural changes in rats. Methods: Adult Sprague-Dawley male rats (n = 18) were randomised into six groups (n = 3/group): i) Control, ii) THSN, iii) KA, iv) TH + KA, v) THSN + KA and vi) Topiramate + KA groups which were pre-treated orally with distilled water, THSN (50 mg/kg body weight), Tualang honey (1.0 g/kg body weight) or Topiramate (40 mg/kg body weight), respectively, five times at 12 h intervals. Saline or KA (15 mg/kg body weight) were injected subcutaneously 30 min after last oral treatment. The latency to onset of the first generalised seizures (FGS) was recorded. Behavioural assessments such as open field test (OFT) to assess the locomotor activity and novel object recognition test (NORT) for learning and memory were performed after 24 h of KA administration. Results: On KA treatment, FGS was observed. But there is a trend of amelioration of FGS on TH+KA and THSN+KA groups when compared to KA group. Pretreatment with TH and THSN improved learning and memory as evidenced by increased recognition index in NORT when compared to untreated KA group. Interestingly, OFT results of THSN+KA group improved the locomotor activity when compared to untreated KA group. Conclusion: These findings suggest that pre-treatment with TH and THSN provides protective effect on KA mediated behavioural changes in male rats. However, further study required to understand the mechanism of action. Penerbit Universiti Sains Malaysia 2020-04-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/81960/1/01MJMS27si2020.pdf Hasim, Hidani and Zulkifli, Nurdarina Ausi and Asari, Mohd Asnizam and Muthuraju, Sangu and Visweswara Rao, Pasupuleti Visweswara and Mustapha, Muzaimi and Kuttulebbai Naina Mohamed Salam, Sirajudeen (2020) Preliminary study on the effect of Tualang honey and its silver nanoparticles on Kainic acid mediated behavioural changes in male rats. Malaysian Journal of Medical Sciences, 27 (Special Issue). pp. 18-19. ISSN 1394-195X E-ISSN 2180-4303 http://www.mjms.usm.my/MJMS27si2020/01MJMS27si2020.pdf |
| spellingShingle | R Medicine (General) Hasim, Hidani Zulkifli, Nurdarina Ausi Asari, Mohd Asnizam Muthuraju, Sangu Visweswara Rao, Pasupuleti Visweswara Mustapha, Muzaimi Kuttulebbai Naina Mohamed Salam, Sirajudeen Preliminary study on the effect of Tualang honey and its silver nanoparticles on Kainic acid mediated behavioural changes in male rats |
| title | Preliminary study on the effect of Tualang honey and its silver nanoparticles on Kainic acid mediated behavioural changes in male rats |
| title_full | Preliminary study on the effect of Tualang honey and its silver nanoparticles on Kainic acid mediated behavioural changes in male rats |
| title_fullStr | Preliminary study on the effect of Tualang honey and its silver nanoparticles on Kainic acid mediated behavioural changes in male rats |
| title_full_unstemmed | Preliminary study on the effect of Tualang honey and its silver nanoparticles on Kainic acid mediated behavioural changes in male rats |
| title_short | Preliminary study on the effect of Tualang honey and its silver nanoparticles on Kainic acid mediated behavioural changes in male rats |
| title_sort | preliminary study on the effect of tualang honey and its silver nanoparticles on kainic acid mediated behavioural changes in male rats |
| topic | R Medicine (General) |
| url | http://irep.iium.edu.my/81960/ http://irep.iium.edu.my/81960/ http://irep.iium.edu.my/81960/1/01MJMS27si2020.pdf |