Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth
Cell suspension culture of Citrus suhuiensis (C. suhuiensis) was established to determine the best growth condition based on the effects of carbon source, plant growth regulators (PGRs) and inoculum size. Friable callus from the cotyledon explant was used to initiate the cell suspension cultures. Mu...
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| Format: | Article |
| Language: | English |
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Journal of the Malaysian Society of Applied Biology
2020
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| Online Access: | http://irep.iium.edu.my/81881/ http://irep.iium.edu.my/81881/1/81881_Effects%20of%20carbon%20sources%2C%20plant%20growth%20regulators%20and%20inoculum%20size.pdf |
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| author | Mohamad Puad, Noor Illi M. Fathil, Nur Alia Amid, Azura Azmi, Azlin Suhaida |
| author_facet | Mohamad Puad, Noor Illi M. Fathil, Nur Alia Amid, Azura Azmi, Azlin Suhaida |
| author_sort | Mohamad Puad, Noor Illi |
| building | IIUM Repository |
| collection | Online Access |
| description | Cell suspension culture of Citrus suhuiensis (C. suhuiensis) was established to determine the best growth condition based on the effects of carbon source, plant growth regulators (PGRs) and inoculum size. Friable callus from the cotyledon explant was used to initiate the cell suspension cultures. Murashige and Skoog (MS) medium supplemented with 50 g/L glucose achieved the highest cell dry weight (CDW) and μmax of 23.2 g/L and 0.47 h-1 respectively compared to glucose at 30 g/L and other carbon sources. For PGRs effects, 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) gave the highest amount of CDW and μmax; 11.91 g/L and 0.32 h-1. Furthermore, the inoculum size at 10% (v/v) gave the maximum CDW and μmax of 15.45 g/L and 0.71 h-1 respectively compared to 20% (v/v) and 30% (v/v) of inoculum. The results achieved can be used for further statistical optimization of C. suhuiensis cell suspension culture growth parameters in which the interactions between
those parameters can be further studied and understood. |
| first_indexed | 2025-11-14T17:52:54Z |
| format | Article |
| id | iium-81881 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:52:54Z |
| publishDate | 2020 |
| publisher | Journal of the Malaysian Society of Applied Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-818812020-08-03T04:44:16Z http://irep.iium.edu.my/81881/ Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth Mohamad Puad, Noor Illi M. Fathil, Nur Alia Amid, Azura Azmi, Azlin Suhaida SB Plant culture TP248.13 Biotechnology Cell suspension culture of Citrus suhuiensis (C. suhuiensis) was established to determine the best growth condition based on the effects of carbon source, plant growth regulators (PGRs) and inoculum size. Friable callus from the cotyledon explant was used to initiate the cell suspension cultures. Murashige and Skoog (MS) medium supplemented with 50 g/L glucose achieved the highest cell dry weight (CDW) and μmax of 23.2 g/L and 0.47 h-1 respectively compared to glucose at 30 g/L and other carbon sources. For PGRs effects, 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) gave the highest amount of CDW and μmax; 11.91 g/L and 0.32 h-1. Furthermore, the inoculum size at 10% (v/v) gave the maximum CDW and μmax of 15.45 g/L and 0.71 h-1 respectively compared to 20% (v/v) and 30% (v/v) of inoculum. The results achieved can be used for further statistical optimization of C. suhuiensis cell suspension culture growth parameters in which the interactions between those parameters can be further studied and understood. Journal of the Malaysian Society of Applied Biology 2020-06-30 Article PeerReviewed application/pdf en http://irep.iium.edu.my/81881/1/81881_Effects%20of%20carbon%20sources%2C%20plant%20growth%20regulators%20and%20inoculum%20size.pdf Mohamad Puad, Noor Illi and M. Fathil, Nur Alia and Amid, Azura and Azmi, Azlin Suhaida (2020) Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth. Malaysia Applied Biology Journal, 49 (1). pp. 87-93. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=981&catid=59:current-view&Itemid=56 |
| spellingShingle | SB Plant culture TP248.13 Biotechnology Mohamad Puad, Noor Illi M. Fathil, Nur Alia Amid, Azura Azmi, Azlin Suhaida Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title | Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title_full | Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title_fullStr | Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title_full_unstemmed | Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title_short | Effects of carbon sources, plant growth regulators and inoculum size on Citrus suhuiensis cell suspension culture growth |
| title_sort | effects of carbon sources, plant growth regulators and inoculum size on citrus suhuiensis cell suspension culture growth |
| topic | SB Plant culture TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/81881/ http://irep.iium.edu.my/81881/ http://irep.iium.edu.my/81881/1/81881_Effects%20of%20carbon%20sources%2C%20plant%20growth%20regulators%20and%20inoculum%20size.pdf |