New lipase producing Betaproteobacteria strains of Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian hotsprings
Microbial lipolytic enzymes have attracted considerable attention owing to their biotechnological potential. In this study, slightly thermophilic bacteria producing lipase were isolated from Bentong and Sungai Lembing hot springs, Pahang, Malaysia. Out of 25 colonies isolated, 14 samples showed t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Malayan Springer Nature Society
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/81879/ http://irep.iium.edu.my/81879/1/MalayanNatureJ_hotspring_Azim.pdf |
| Summary: | Microbial lipolytic enzymes have attracted considerable attention owing to their
biotechnological potential. In this study, slightly thermophilic bacteria producing lipase were
isolated from Bentong and Sungai Lembing hot springs, Pahang, Malaysia. Out of 25 colonies
isolated, 14 samples showed to produce clear zones surrounding the growth on tributyrin
agar plates. All 14 isolates showed Gram-negative bacteria with short rod morphology. PCR
amplification of 16S ribosomal DNA gene showed that these isolates were clustered with subclass
Betaproteobacteria consisting of slightly thermophilic bacteria that produce lipase. Phylogenetic
analysis was carried out with the highly similar species and four isolates (SglA1, BtnC1, BtnC2
and BtnC3) are related to genus Caldimonas and 10 isolates (SglB1, SglB2, SglB3, SglB4, BtnB1,
BtnB2 BtnD1, BtnD2, BtnD3 and BtnD4) belonged to Tepidimonas. These results indicated that
novel lipase-producing thermophilic Betaproteobacteria could be isolated from these hot springs. |
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