Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics
Fruit of salak (Salacca zalacca) is tradition ally used and commercialized as an an tidiabetic agent. How ever, the scientific evidence to prove this traditional use is lack ing. This re search was aimed to evaluate the metabolic changes of obese-diabetic (OBDC) rats treated with S. zalacca fruit...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English English English |
| Published: |
Elseviers
2020
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/81676/ http://irep.iium.edu.my/81676/7/evidence%20email-jamshed.pdf http://irep.iium.edu.my/81676/13/1-s2.0-S096399692030572X-main.pdf http://irep.iium.edu.my/81676/19/81676_Modulation%20of%20metabolic%20alterations%20of%20obese_scopus.pdf http://irep.iium.edu.my/81676/25/81676_Modulation%20of%20metabolic%20alterations%20of%20obese%20diabetic%20rats_WoS.pdf |
| Summary: | Fruit of salak (Salacca zalacca) is tradition ally used and commercialized as an an tidiabetic agent. How ever,
the scientific evidence to prove this traditional use is lack ing. This re search was aimed to evaluate the
metabolic changes of obese-diabetic (OBDC) rats treated with S. zalacca fruit using proton-nuclear magnetic
resonance ( H NMR)-based metabolomics approach. This re search presents the first report on the in vitro
antidiabetic effect of S. zalacca fruits ex tract us ing this approach. The obtained results in dicated that the
administration of 400 mg/ kg bw of 60% ethanolic S. zalacca extract for 6 weeks significantly de creased the
blood glucose level and normalized the blood lipid pro file of the OBDC rats. The potential biomarkers in
urine were 2-oxoglutarate, alanine, leucine, succinate 3-hydroxy butyrate, taurine, betaine, allantoin, acetate, dimethylamine, creatine, creatinine, glucose, phenyl-acetyl glycine, and hippurate. Based on the data
obtained, the metabolite pro files of the urine of treated rats by the 60% ethanolic extract could not be fully
improved the metabolic complications of diabetic rats. The ex tract of S. zalacca fruit was able to de crease
the ketones bodies as 3-hydroxy butyrate and acetoacetate. It has also improved energy metabolism, involving glucose, acetate, lactate, 2-hydroxy butyrate, 2-oxoglutarate, citrate, and succinate. More over, it decreased metabolites from gut microflora, including choline. This extract had significant effect on amino acid metabolism, metabolites from gut microflora, bile acid metabolism and creatine. The result can further
support the traditional claims of S. zalacca fruits in management of diabetes. This finding might bevaluable
in understanding the molecular mechanism and pharmacological properties of this medicinal plant for
managing diabetes mellitus. |
|---|