Angiotensin II type 1 receptor (AGTR1) methylation is inversely associated with HbA1c level in non-diabetic young adults
Introduction: The increasing incidence of early onset type 2 diabetes worldwide is worrying. Epigenetics may play a role in the pathophysiology of insulin resistance and glucose intolerance. It is hypothesized that overexpression of angiotensin II type 1 receptor (AGTR1) in pancreas impairs insulin...
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| Format: | Proceeding Paper |
| Language: | English English |
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2019
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| Online Access: | http://irep.iium.edu.my/81675/ http://irep.iium.edu.my/81675/7/poster%20COSS.pdf http://irep.iium.edu.my/81675/12/Biomedical%20Cambridge-Oxford-Sunway%20Symposium%20program%20book.pdf |
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| author | Wan Omar, Wan Fatein Nabeila A.Talib, Norlelawati Mohd. Shah, Aida Nur Sharini Mohd Shah, Azarisman Shah Abdullah, Aszrin |
| author_facet | Wan Omar, Wan Fatein Nabeila A.Talib, Norlelawati Mohd. Shah, Aida Nur Sharini Mohd Shah, Azarisman Shah Abdullah, Aszrin |
| author_sort | Wan Omar, Wan Fatein Nabeila |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: The increasing incidence of early onset type 2 diabetes worldwide is worrying. Epigenetics may play a role in the pathophysiology of insulin resistance and glucose intolerance. It is hypothesized that overexpression of angiotensin II type 1 receptor (AGTR1) in pancreas impairs insulin sensitivity and insulin secretion, explaining the improved glycaemic control in diabetic patients on angiotensin receptor blocker. Methylation changes in pancreas may be parallel with peripheral blood. To date, the association between AGTR1 methylation and glycemic control has never been investigated. Therefore, the aim of this study is to investigate the association between AGTR1 promoter methylation level and glycaemic control parameters in non-diabetic young adults.
Methods: An observational cross-sectional study involving 240 non-diabetic young adults age 18 to 45 years were conducted in Kuantan, Pahang. Subjects were examined for fasting blood glucose (FBG) level, HBA1c level and DNA methylation of AGTR1 promoter of the peripheral blood using MethyLight TaqMan assay. Bivariate and multivariate analysis was conducted to determine the association.
Results: AGTR1 methylation was inversely associated with HBA1c (p=0.012, r=-0.173), but not with FBG (p=0.622). The association between AGTR1 methylation and HBA1c remained significant after adjusting to age and sex (p=0.008, =-2.660).
Conclusions: AGTR methylation is inversely associated with HBA1c level in non-diabetic young adults, hence may be involved in the pathogenesis of insulin resistance. AGTR1 gene expression should follow to determine if AGTR1 hypermethylation is associated with lower AGTR gene expression which could explain the association. |
| first_indexed | 2025-11-14T17:52:23Z |
| format | Proceeding Paper |
| id | iium-81675 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:52:23Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-816752020-08-04T06:07:49Z http://irep.iium.edu.my/81675/ Angiotensin II type 1 receptor (AGTR1) methylation is inversely associated with HbA1c level in non-diabetic young adults Wan Omar, Wan Fatein Nabeila A.Talib, Norlelawati Mohd. Shah, Aida Nur Sharini Mohd Shah, Azarisman Shah Abdullah, Aszrin QH426 Genetics QP Physiology Introduction: The increasing incidence of early onset type 2 diabetes worldwide is worrying. Epigenetics may play a role in the pathophysiology of insulin resistance and glucose intolerance. It is hypothesized that overexpression of angiotensin II type 1 receptor (AGTR1) in pancreas impairs insulin sensitivity and insulin secretion, explaining the improved glycaemic control in diabetic patients on angiotensin receptor blocker. Methylation changes in pancreas may be parallel with peripheral blood. To date, the association between AGTR1 methylation and glycemic control has never been investigated. Therefore, the aim of this study is to investigate the association between AGTR1 promoter methylation level and glycaemic control parameters in non-diabetic young adults. Methods: An observational cross-sectional study involving 240 non-diabetic young adults age 18 to 45 years were conducted in Kuantan, Pahang. Subjects were examined for fasting blood glucose (FBG) level, HBA1c level and DNA methylation of AGTR1 promoter of the peripheral blood using MethyLight TaqMan assay. Bivariate and multivariate analysis was conducted to determine the association. Results: AGTR1 methylation was inversely associated with HBA1c (p=0.012, r=-0.173), but not with FBG (p=0.622). The association between AGTR1 methylation and HBA1c remained significant after adjusting to age and sex (p=0.008, =-2.660). Conclusions: AGTR methylation is inversely associated with HBA1c level in non-diabetic young adults, hence may be involved in the pathogenesis of insulin resistance. AGTR1 gene expression should follow to determine if AGTR1 hypermethylation is associated with lower AGTR gene expression which could explain the association. 2019-03-26 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/81675/7/poster%20COSS.pdf application/pdf en http://irep.iium.edu.my/81675/12/Biomedical%20Cambridge-Oxford-Sunway%20Symposium%20program%20book.pdf Wan Omar, Wan Fatein Nabeila and A.Talib, Norlelawati and Mohd. Shah, Aida Nur Sharini and Mohd Shah, Azarisman Shah and Abdullah, Aszrin (2019) Angiotensin II type 1 receptor (AGTR1) methylation is inversely associated with HbA1c level in non-diabetic young adults. In: 3rd Cambridge Oxford Sunway Biomedical Symposium. Diabetes: Disarming The Silent Killer, 26th till 27 March 2019, Sunway Medical Centre, Subang Jaya, Selangor, Malaysia. (Unpublished) |
| spellingShingle | QH426 Genetics QP Physiology Wan Omar, Wan Fatein Nabeila A.Talib, Norlelawati Mohd. Shah, Aida Nur Sharini Mohd Shah, Azarisman Shah Abdullah, Aszrin Angiotensin II type 1 receptor (AGTR1) methylation is inversely associated with HbA1c level in non-diabetic young adults |
| title | Angiotensin II type 1 receptor (AGTR1) methylation is inversely associated with HbA1c level in non-diabetic young adults |
| title_full | Angiotensin II type 1 receptor (AGTR1) methylation is inversely associated with HbA1c level in non-diabetic young adults |
| title_fullStr | Angiotensin II type 1 receptor (AGTR1) methylation is inversely associated with HbA1c level in non-diabetic young adults |
| title_full_unstemmed | Angiotensin II type 1 receptor (AGTR1) methylation is inversely associated with HbA1c level in non-diabetic young adults |
| title_short | Angiotensin II type 1 receptor (AGTR1) methylation is inversely associated with HbA1c level in non-diabetic young adults |
| title_sort | angiotensin ii type 1 receptor (agtr1) methylation is inversely associated with hba1c level in non-diabetic young adults |
| topic | QH426 Genetics QP Physiology |
| url | http://irep.iium.edu.my/81675/ http://irep.iium.edu.my/81675/7/poster%20COSS.pdf http://irep.iium.edu.my/81675/12/Biomedical%20Cambridge-Oxford-Sunway%20Symposium%20program%20book.pdf |