Lactococcus lactis strains from intestinal organ of black tips shark Carcharhinus limbatus producing nisin-like bacteriocin active against Shrimp and fish pathogens (vibrio parahaemolyticus and vibrio alginolyticus)
Shark can be a potential source for host-associated probiotics destined to be used in aquaculture. In this study, intestinal tissues of Black tip shark (Carcharhinus limbatus) was used as the source sample for inhibition screening based on spot-on-lawn and agar well diffusion methods. Out of the 8...
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| Format: | Article |
| Language: | English English |
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Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture
2020
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| Online Access: | http://irep.iium.edu.my/81603/ http://irep.iium.edu.my/81603/1/81603_Lactococcus%20lactis%20strains%20from%20intestinal%20organ.pdf http://irep.iium.edu.my/81603/2/81603_Lactococcus%20lactis%20strains%20from%20intestinal%20organ_SCOPUS.pdf |
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| author | Tengku Abdul Hamid, Tengku Haziyamin Ahmad Zahli, Khairil Ikhwan Mohammed, Alotaibi |
| author_facet | Tengku Abdul Hamid, Tengku Haziyamin Ahmad Zahli, Khairil Ikhwan Mohammed, Alotaibi |
| author_sort | Tengku Abdul Hamid, Tengku Haziyamin |
| building | IIUM Repository |
| collection | Online Access |
| description | Shark can be a potential source for host-associated probiotics destined to be used in aquaculture. In this study, intestinal tissues of Black tip
shark (Carcharhinus limbatus) was used as the source sample for inhibition screening based on spot-on-lawn and agar well diffusion methods.
Out of the 80 isolates, only four showed antagonistic activities against selected indicator strains. All of the 4 isolates FA1, FA2, FA3 and FA4
were found to be Gram positive coccus, non-spore former, oxidase and catalase negatives, as well as lactose fermenters. The isolate FA1,
FA2, FA3 and FA4 were found to demonstrate broad range of inhibitory spectrum on gram-positive indicator bacterium (Staphylococcus
aureus and Bacillus cereus) and the gram-negative bacterium (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Vibrio
alginolyticus and Vibrio parahaemolyticus); with the highest inhibition zone at 20.0±0.1 mm, recorded on V. parahaemolyticus. The genotype
of the isolates was characterized using 16S rRNA sequencing. Each sequence with a given GenBank (NCBI) accession number (MN975529
for FA1; MN982712 for FA2; MN982711 for FA3; and MN982710 for FA4) showed at least 99% similarity with Lactococcus lactis
subspecies. The crude cell free supernatant (CFS) of the isolates potentially contained a putative bacteriocin displaying nisin-like properties.
The pH and catalase treatments showed that neither organic acid nor hydrogen peroxide (H2O2) was the inhibitory component. An almost 95%
inactivation of the CFS’s antimicrobial activity was observed following treatment with trypsin suggested a presence of proteinaceous agent.
Growth and inhibition studies on all isolated strains demonstrated that the inhibition properties were growth associated, with maximum
inhibition achieved at ~20.0 ± 0.1 mm (or ~168 ± 17 AU/ml) at 17 hours by FA2 strain against V. parahaemolyticus. A study on the mode of
inhibition demonstrated a bactericidal killing against V. parahaemolyticus. The isolation of Lactococcus lactis strain from shark is rather
unique since it was commonly reported to be isolated from human and animals. These strains show bacteriocinogenic properties, broad
spectrum inhibition, and more importantly, they are able to antagonise some pathogens implicated in fish or shrimp diseases. Therefore, these
strains have potential for use as probiotics in aquaculture. |
| first_indexed | 2025-11-14T17:52:15Z |
| format | Article |
| id | iium-81603 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:52:15Z |
| publishDate | 2020 |
| publisher | Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-816032021-12-15T00:25:53Z http://irep.iium.edu.my/81603/ Lactococcus lactis strains from intestinal organ of black tips shark Carcharhinus limbatus producing nisin-like bacteriocin active against Shrimp and fish pathogens (vibrio parahaemolyticus and vibrio alginolyticus) Tengku Abdul Hamid, Tengku Haziyamin Ahmad Zahli, Khairil Ikhwan Mohammed, Alotaibi Q Science (General) QR Microbiology Shark can be a potential source for host-associated probiotics destined to be used in aquaculture. In this study, intestinal tissues of Black tip shark (Carcharhinus limbatus) was used as the source sample for inhibition screening based on spot-on-lawn and agar well diffusion methods. Out of the 80 isolates, only four showed antagonistic activities against selected indicator strains. All of the 4 isolates FA1, FA2, FA3 and FA4 were found to be Gram positive coccus, non-spore former, oxidase and catalase negatives, as well as lactose fermenters. The isolate FA1, FA2, FA3 and FA4 were found to demonstrate broad range of inhibitory spectrum on gram-positive indicator bacterium (Staphylococcus aureus and Bacillus cereus) and the gram-negative bacterium (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Vibrio alginolyticus and Vibrio parahaemolyticus); with the highest inhibition zone at 20.0±0.1 mm, recorded on V. parahaemolyticus. The genotype of the isolates was characterized using 16S rRNA sequencing. Each sequence with a given GenBank (NCBI) accession number (MN975529 for FA1; MN982712 for FA2; MN982711 for FA3; and MN982710 for FA4) showed at least 99% similarity with Lactococcus lactis subspecies. The crude cell free supernatant (CFS) of the isolates potentially contained a putative bacteriocin displaying nisin-like properties. The pH and catalase treatments showed that neither organic acid nor hydrogen peroxide (H2O2) was the inhibitory component. An almost 95% inactivation of the CFS’s antimicrobial activity was observed following treatment with trypsin suggested a presence of proteinaceous agent. Growth and inhibition studies on all isolated strains demonstrated that the inhibition properties were growth associated, with maximum inhibition achieved at ~20.0 ± 0.1 mm (or ~168 ± 17 AU/ml) at 17 hours by FA2 strain against V. parahaemolyticus. A study on the mode of inhibition demonstrated a bactericidal killing against V. parahaemolyticus. The isolation of Lactococcus lactis strain from shark is rather unique since it was commonly reported to be isolated from human and animals. These strains show bacteriocinogenic properties, broad spectrum inhibition, and more importantly, they are able to antagonise some pathogens implicated in fish or shrimp diseases. Therefore, these strains have potential for use as probiotics in aquaculture. Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture 2020-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/81603/1/81603_Lactococcus%20lactis%20strains%20from%20intestinal%20organ.pdf application/pdf en http://irep.iium.edu.my/81603/2/81603_Lactococcus%20lactis%20strains%20from%20intestinal%20organ_SCOPUS.pdf Tengku Abdul Hamid, Tengku Haziyamin and Ahmad Zahli, Khairil Ikhwan and Mohammed, Alotaibi (2020) Lactococcus lactis strains from intestinal organ of black tips shark Carcharhinus limbatus producing nisin-like bacteriocin active against Shrimp and fish pathogens (vibrio parahaemolyticus and vibrio alginolyticus). Journal of Microbiology, Biotechnology & Food Science, 10 (3). ISSN 1338-5178 https://office2.jmbfs.org/index.php/JMBFS/article/view/3055/83 doi:10.15414/jmbfs.2020.10.3.354-360 |
| spellingShingle | Q Science (General) QR Microbiology Tengku Abdul Hamid, Tengku Haziyamin Ahmad Zahli, Khairil Ikhwan Mohammed, Alotaibi Lactococcus lactis strains from intestinal organ of black tips shark Carcharhinus limbatus producing nisin-like bacteriocin active against Shrimp and fish pathogens (vibrio parahaemolyticus and vibrio alginolyticus) |
| title | Lactococcus lactis strains from intestinal organ of black tips shark Carcharhinus limbatus producing nisin-like bacteriocin active against
Shrimp and fish pathogens (vibrio parahaemolyticus and vibrio alginolyticus) |
| title_full | Lactococcus lactis strains from intestinal organ of black tips shark Carcharhinus limbatus producing nisin-like bacteriocin active against
Shrimp and fish pathogens (vibrio parahaemolyticus and vibrio alginolyticus) |
| title_fullStr | Lactococcus lactis strains from intestinal organ of black tips shark Carcharhinus limbatus producing nisin-like bacteriocin active against
Shrimp and fish pathogens (vibrio parahaemolyticus and vibrio alginolyticus) |
| title_full_unstemmed | Lactococcus lactis strains from intestinal organ of black tips shark Carcharhinus limbatus producing nisin-like bacteriocin active against
Shrimp and fish pathogens (vibrio parahaemolyticus and vibrio alginolyticus) |
| title_short | Lactococcus lactis strains from intestinal organ of black tips shark Carcharhinus limbatus producing nisin-like bacteriocin active against
Shrimp and fish pathogens (vibrio parahaemolyticus and vibrio alginolyticus) |
| title_sort | lactococcus lactis strains from intestinal organ of black tips shark carcharhinus limbatus producing nisin-like bacteriocin active against
shrimp and fish pathogens (vibrio parahaemolyticus and vibrio alginolyticus) |
| topic | Q Science (General) QR Microbiology |
| url | http://irep.iium.edu.my/81603/ http://irep.iium.edu.my/81603/ http://irep.iium.edu.my/81603/ http://irep.iium.edu.my/81603/1/81603_Lactococcus%20lactis%20strains%20from%20intestinal%20organ.pdf http://irep.iium.edu.my/81603/2/81603_Lactococcus%20lactis%20strains%20from%20intestinal%20organ_SCOPUS.pdf |