Endophytic bacteria of Anisophyllea disticha (Raja Berangkat) from tropical lake environment in Malaysia

Lake Kenyir is the largest artificial lake in South East Asia and serves as habitats for diverse flora and fauna. Moreover, Lake Kenyir is a rich resource of medicinal plants which has been used by local community surrounding the lake. Endophytic bacteria are able to colonize plants without causing...

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Main Authors: Zainal Abidin, Zaima Azira, Zolkeflia, Nurhasliza, Shahari, Rozilawati, Khan Chowdhury, Ahmed Jalal
Format: Article
Language:English
Published: Desalination Publication 2020
Subjects:
Online Access:http://irep.iium.edu.my/81479/
http://irep.iium.edu.my/81479/1/ENDOPHYTIC%20BACTERIA.pdf
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author Zainal Abidin, Zaima Azira
Zolkeflia, Nurhasliza
Shahari, Rozilawati
Khan Chowdhury, Ahmed Jalal
author_facet Zainal Abidin, Zaima Azira
Zolkeflia, Nurhasliza
Shahari, Rozilawati
Khan Chowdhury, Ahmed Jalal
author_sort Zainal Abidin, Zaima Azira
building IIUM Repository
collection Online Access
description Lake Kenyir is the largest artificial lake in South East Asia and serves as habitats for diverse flora and fauna. Moreover, Lake Kenyir is a rich resource of medicinal plants which has been used by local community surrounding the lake. Endophytic bacteria are able to colonize plants without causing harm and they can be highly beneficial towards their plant hosts. The aim of this study is to isolate and identify endophytic bacteria from the leaves and roots of a medicinal plant, Anisophyllea disticha (Raja Berangkat), and to evaluate their antimicrobial capabilities. Twenty isolates each from the leaves and roots were randomly selected for molecular identification using 16S rRNA gene. Partial 16S rRNA gene sequences revealed that genus Bacillus dominated the endophytic bacteria from the leaves with two isolates belonging to Paenibacillus and Aeribacillus. Five genera namely, Bacillus, Staphylococcus, Lysinibacillus, Paenibacillus, and Mycobacterium were successfully obtained from the roots of A. disticha. Nine endophytic bacteria—five Bacillus species (R3, R11, R13, R26, R37), Lysinibacillus (R9), two Staphylococcus species (R6, R27) and Mycobacterium species (R33) from roots were found to show weak antibacterial and/or antifungal activities. Findings from this study provide an insight to endophytic bacteria inhabiting A. disticha and the role they may play in their plant host.
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institution International Islamic University Malaysia
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spelling iium-814792020-10-28T03:48:26Z http://irep.iium.edu.my/81479/ Endophytic bacteria of Anisophyllea disticha (Raja Berangkat) from tropical lake environment in Malaysia Zainal Abidin, Zaima Azira Zolkeflia, Nurhasliza Shahari, Rozilawati Khan Chowdhury, Ahmed Jalal QR Microbiology Lake Kenyir is the largest artificial lake in South East Asia and serves as habitats for diverse flora and fauna. Moreover, Lake Kenyir is a rich resource of medicinal plants which has been used by local community surrounding the lake. Endophytic bacteria are able to colonize plants without causing harm and they can be highly beneficial towards their plant hosts. The aim of this study is to isolate and identify endophytic bacteria from the leaves and roots of a medicinal plant, Anisophyllea disticha (Raja Berangkat), and to evaluate their antimicrobial capabilities. Twenty isolates each from the leaves and roots were randomly selected for molecular identification using 16S rRNA gene. Partial 16S rRNA gene sequences revealed that genus Bacillus dominated the endophytic bacteria from the leaves with two isolates belonging to Paenibacillus and Aeribacillus. Five genera namely, Bacillus, Staphylococcus, Lysinibacillus, Paenibacillus, and Mycobacterium were successfully obtained from the roots of A. disticha. Nine endophytic bacteria—five Bacillus species (R3, R11, R13, R26, R37), Lysinibacillus (R9), two Staphylococcus species (R6, R27) and Mycobacterium species (R33) from roots were found to show weak antibacterial and/or antifungal activities. Findings from this study provide an insight to endophytic bacteria inhabiting A. disticha and the role they may play in their plant host. Desalination Publication 2020-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/81479/1/ENDOPHYTIC%20BACTERIA.pdf Zainal Abidin, Zaima Azira and Zolkeflia, Nurhasliza and Shahari, Rozilawati and Khan Chowdhury, Ahmed Jalal (2020) Endophytic bacteria of Anisophyllea disticha (Raja Berangkat) from tropical lake environment in Malaysia. Desalination and Water Treatment, 188. pp. 282-287. ISSN 1944-3994 E-ISSN 1944-3986 https://www.deswater.com/home.php
spellingShingle QR Microbiology
Zainal Abidin, Zaima Azira
Zolkeflia, Nurhasliza
Shahari, Rozilawati
Khan Chowdhury, Ahmed Jalal
Endophytic bacteria of Anisophyllea disticha (Raja Berangkat) from tropical lake environment in Malaysia
title Endophytic bacteria of Anisophyllea disticha (Raja Berangkat) from tropical lake environment in Malaysia
title_full Endophytic bacteria of Anisophyllea disticha (Raja Berangkat) from tropical lake environment in Malaysia
title_fullStr Endophytic bacteria of Anisophyllea disticha (Raja Berangkat) from tropical lake environment in Malaysia
title_full_unstemmed Endophytic bacteria of Anisophyllea disticha (Raja Berangkat) from tropical lake environment in Malaysia
title_short Endophytic bacteria of Anisophyllea disticha (Raja Berangkat) from tropical lake environment in Malaysia
title_sort endophytic bacteria of anisophyllea disticha (raja berangkat) from tropical lake environment in malaysia
topic QR Microbiology
url http://irep.iium.edu.my/81479/
http://irep.iium.edu.my/81479/
http://irep.iium.edu.my/81479/1/ENDOPHYTIC%20BACTERIA.pdf