Amniotic membrane enhance the effect of vascular endothelial growth factor on the angiogenic marker expression of stem cells from human exfoliated deciduous teeth
Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this in vitro work was to study the effect of 2...
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| Format: | Article |
| Language: | English English English |
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Springer
2020
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| Online Access: | http://irep.iium.edu.my/81074/ http://irep.iium.edu.my/81074/19/81074_Amniotic%20membrane%20enhance%20the%20effect_MyRA.pdf http://irep.iium.edu.my/81074/7/81074_Amniotic%20membrane%20enhance%20the%20effect_SCOPUS.pdf http://irep.iium.edu.my/81074/8/81074_Amniotic%20membrane%20enhance%20the%20effect_WOS.pdf |
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| author | Yusof, Muhammad Fuad Hilmi Md Hashim, Siti Nurnasihah Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Thirumulu Ponnuraj, Kannan Sheikh Ab. Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina |
| author_facet | Yusof, Muhammad Fuad Hilmi Md Hashim, Siti Nurnasihah Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Thirumulu Ponnuraj, Kannan Sheikh Ab. Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina |
| author_sort | Yusof, Muhammad Fuad Hilmi |
| building | IIUM Repository |
| collection | Online Access |
| description | Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this
in vitro work was to study the effect of 24-h VEGF induced on SHED endothelial differentiation when seeded on acellular stromal side (SS) of AM matrix. Stemness of
SHED was identified by flow cytometry. Cell attachment and morphological changes towards the matrix was observed by scanning electron microscopy. Protein expression of endothelial marker was examined by Western blot. The expression of stem cells and endothelial-specific gene markers of VEGF-induced SHED cultured on human AM was inspected via reverse transcriptase-polymerase chain reaction. Results showed SHED at both passages retain stemness property. Ang-1 protein was expressed in SHED. Cells treated with VEGF and cultured on AM transformed attached well to AM. VEGF induced SHED expressed both stem cell and endothelial-specific markers throughout the treatments and timeline. Interestingly, prolonged VEGF treatment increased the expression of Cox-2 and VE-Cadherin genes in all treated groups when compared to SHED. It was concluded that the VEGF-induced SHED showed better expression of endothelial-specific markers when cultured on SS of AM, with prolonged VEGF treatment. |
| first_indexed | 2025-11-14T17:50:55Z |
| format | Article |
| id | iium-81074 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:50:55Z |
| publishDate | 2020 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-810742020-07-27T06:55:00Z http://irep.iium.edu.my/81074/ Amniotic membrane enhance the effect of vascular endothelial growth factor on the angiogenic marker expression of stem cells from human exfoliated deciduous teeth Yusof, Muhammad Fuad Hilmi Md Hashim, Siti Nurnasihah Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Thirumulu Ponnuraj, Kannan Sheikh Ab. Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina RK Dentistry Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this in vitro work was to study the effect of 24-h VEGF induced on SHED endothelial differentiation when seeded on acellular stromal side (SS) of AM matrix. Stemness of SHED was identified by flow cytometry. Cell attachment and morphological changes towards the matrix was observed by scanning electron microscopy. Protein expression of endothelial marker was examined by Western blot. The expression of stem cells and endothelial-specific gene markers of VEGF-induced SHED cultured on human AM was inspected via reverse transcriptase-polymerase chain reaction. Results showed SHED at both passages retain stemness property. Ang-1 protein was expressed in SHED. Cells treated with VEGF and cultured on AM transformed attached well to AM. VEGF induced SHED expressed both stem cell and endothelial-specific markers throughout the treatments and timeline. Interestingly, prolonged VEGF treatment increased the expression of Cox-2 and VE-Cadherin genes in all treated groups when compared to SHED. It was concluded that the VEGF-induced SHED showed better expression of endothelial-specific markers when cultured on SS of AM, with prolonged VEGF treatment. Springer 2020-02-25 Article PeerReviewed application/pdf en http://irep.iium.edu.my/81074/19/81074_Amniotic%20membrane%20enhance%20the%20effect_MyRA.pdf application/pdf en http://irep.iium.edu.my/81074/7/81074_Amniotic%20membrane%20enhance%20the%20effect_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/81074/8/81074_Amniotic%20membrane%20enhance%20the%20effect_WOS.pdf Yusof, Muhammad Fuad Hilmi and Md Hashim, Siti Nurnasihah and Zahari, Wafa’ and Chandra, Hamshawagini and Ahmad Amin Noordin, Khairul Bariah and Thirumulu Ponnuraj, Kannan and Sheikh Ab. Hamid, Suzina and Mokhtar@Makhtar, Khairani Idah and Ahmad, Azlina (2020) Amniotic membrane enhance the effect of vascular endothelial growth factor on the angiogenic marker expression of stem cells from human exfoliated deciduous teeth. Applied Biochemistry and Biotechnology, 191. pp. 177-190. ISSN 0273-2289 E-ISSN 1559-0291 https://link.springer.com/article/10.1007/s12010-020-03266-1 https://doi.org/10.1007/s12010-020-03266-1 |
| spellingShingle | RK Dentistry Yusof, Muhammad Fuad Hilmi Md Hashim, Siti Nurnasihah Zahari, Wafa’ Chandra, Hamshawagini Ahmad Amin Noordin, Khairul Bariah Thirumulu Ponnuraj, Kannan Sheikh Ab. Hamid, Suzina Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina Amniotic membrane enhance the effect of vascular endothelial growth factor on the angiogenic marker expression of stem cells from human exfoliated deciduous teeth |
| title | Amniotic membrane enhance the effect of vascular
endothelial growth factor on the angiogenic marker
expression of stem cells from human exfoliated
deciduous teeth |
| title_full | Amniotic membrane enhance the effect of vascular
endothelial growth factor on the angiogenic marker
expression of stem cells from human exfoliated
deciduous teeth |
| title_fullStr | Amniotic membrane enhance the effect of vascular
endothelial growth factor on the angiogenic marker
expression of stem cells from human exfoliated
deciduous teeth |
| title_full_unstemmed | Amniotic membrane enhance the effect of vascular
endothelial growth factor on the angiogenic marker
expression of stem cells from human exfoliated
deciduous teeth |
| title_short | Amniotic membrane enhance the effect of vascular
endothelial growth factor on the angiogenic marker
expression of stem cells from human exfoliated
deciduous teeth |
| title_sort | amniotic membrane enhance the effect of vascular
endothelial growth factor on the angiogenic marker
expression of stem cells from human exfoliated
deciduous teeth |
| topic | RK Dentistry |
| url | http://irep.iium.edu.my/81074/ http://irep.iium.edu.my/81074/ http://irep.iium.edu.my/81074/ http://irep.iium.edu.my/81074/19/81074_Amniotic%20membrane%20enhance%20the%20effect_MyRA.pdf http://irep.iium.edu.my/81074/7/81074_Amniotic%20membrane%20enhance%20the%20effect_SCOPUS.pdf http://irep.iium.edu.my/81074/8/81074_Amniotic%20membrane%20enhance%20the%20effect_WOS.pdf |