Genome comparison of Actinomyces naeslundii with known probiotics
Introduction: Actinomyces naeslundii is an oral microbiome that has been suggested to involve in actinomycosis particularly in immunocompromised patients. However, studies have also shown that the bacterium can inhibit the colonisation of Candida albicans and cariogenic bacterium, Streptococcus muta...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IIUM Press
2019
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/80870/ http://irep.iium.edu.my/80870/1/637-Manuscript-1800-1-10-20200116.pdf |
| _version_ | 1848789036213207040 |
|---|---|
| author | Wan Mohd Kamaluddin, Wan Nur Fatihah Ismail, Ahmad Faisal Mohammad Aidid, Edre Othman, Noratikah Arzmi, Mohd Hafiz |
| author_facet | Wan Mohd Kamaluddin, Wan Nur Fatihah Ismail, Ahmad Faisal Mohammad Aidid, Edre Othman, Noratikah Arzmi, Mohd Hafiz |
| author_sort | Wan Mohd Kamaluddin, Wan Nur Fatihah |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Actinomyces naeslundii is an oral microbiome that has been suggested to involve in actinomycosis particularly in immunocompromised patients. However, studies have also shown that the bacterium can inhibit the colonisation of Candida albicans and cariogenic bacterium, Streptococcus mutans. Even though various studies have been conducted to characterise A. naeslundii, however, the role of the bacterium in the oral cavity either to induce pathogenicity or to improve oral health remain unclear. The objective of this study is to determine the role of A. naeslundii in the oral cavity using bioinformatic tools with the hypothesis that A. naeslundii possesses genome similarity to oral probiotics. Materials and method: The study was conducted by data mining of the genomic sequences of A. naeslundii NCTC10301 from GenBank. Following that, genomic comparison was performed with 25 species of well-known probiotics. MAUVE version 2.3.1 was used to find similarities between A. naeslundii with the known probiotics through identification of potential relevance probiotic properties in A. naeslundii. Phylogenetic tree was constructed using PHYLIP package to identify evolutionary relationship of A. naeslundii with the known probiotics. The 16s rRNA sequences of A. naeslundii and known probiotics were harvested from GenBank using BLAST. Finally, the functions of all A. naeslundii genes that shared similarity with known probiotics were identified using INTERPRO. Result: Genome comparison analysis of the present study showed that A. naeslundii genome exhibited high similarity to Bifidobacterium species such as B. animalis subsp. lactis, B. bifidum, B. longum and B. breve. In addition, phylogenetic tree analysis showed that Bacillus species, B. subtilis and B. cereus , appeared to clade together with A. naeslundii, with bootstrap value of 98%. Conclusion: A. naeslundii had high similarity with probiotic Bifidobacterium species thus supported the hypothesis of the present study that A. naeslundii possesses genome similarity to oral probiotic. |
| first_indexed | 2025-11-14T17:50:20Z |
| format | Article |
| id | iium-80870 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:50:20Z |
| publishDate | 2019 |
| publisher | IIUM Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-808702020-07-01T04:07:29Z http://irep.iium.edu.my/80870/ Genome comparison of Actinomyces naeslundii with known probiotics Wan Mohd Kamaluddin, Wan Nur Fatihah Ismail, Ahmad Faisal Mohammad Aidid, Edre Othman, Noratikah Arzmi, Mohd Hafiz QR Microbiology RK Dentistry Introduction: Actinomyces naeslundii is an oral microbiome that has been suggested to involve in actinomycosis particularly in immunocompromised patients. However, studies have also shown that the bacterium can inhibit the colonisation of Candida albicans and cariogenic bacterium, Streptococcus mutans. Even though various studies have been conducted to characterise A. naeslundii, however, the role of the bacterium in the oral cavity either to induce pathogenicity or to improve oral health remain unclear. The objective of this study is to determine the role of A. naeslundii in the oral cavity using bioinformatic tools with the hypothesis that A. naeslundii possesses genome similarity to oral probiotics. Materials and method: The study was conducted by data mining of the genomic sequences of A. naeslundii NCTC10301 from GenBank. Following that, genomic comparison was performed with 25 species of well-known probiotics. MAUVE version 2.3.1 was used to find similarities between A. naeslundii with the known probiotics through identification of potential relevance probiotic properties in A. naeslundii. Phylogenetic tree was constructed using PHYLIP package to identify evolutionary relationship of A. naeslundii with the known probiotics. The 16s rRNA sequences of A. naeslundii and known probiotics were harvested from GenBank using BLAST. Finally, the functions of all A. naeslundii genes that shared similarity with known probiotics were identified using INTERPRO. Result: Genome comparison analysis of the present study showed that A. naeslundii genome exhibited high similarity to Bifidobacterium species such as B. animalis subsp. lactis, B. bifidum, B. longum and B. breve. In addition, phylogenetic tree analysis showed that Bacillus species, B. subtilis and B. cereus , appeared to clade together with A. naeslundii, with bootstrap value of 98%. Conclusion: A. naeslundii had high similarity with probiotic Bifidobacterium species thus supported the hypothesis of the present study that A. naeslundii possesses genome similarity to oral probiotic. IIUM Press 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/80870/1/637-Manuscript-1800-1-10-20200116.pdf Wan Mohd Kamaluddin, Wan Nur Fatihah and Ismail, Ahmad Faisal and Mohammad Aidid, Edre and Othman, Noratikah and Arzmi, Mohd Hafiz (2019) Genome comparison of Actinomyces naeslundii with known probiotics. IIUM Medical Journal of Malaysia, 18 (Supplementary Issue 2). p. 58. E-ISSN 1823-4631 https://journals.iium.edu.my/kom/index.php/imjm/article/view/637/396 |
| spellingShingle | QR Microbiology RK Dentistry Wan Mohd Kamaluddin, Wan Nur Fatihah Ismail, Ahmad Faisal Mohammad Aidid, Edre Othman, Noratikah Arzmi, Mohd Hafiz Genome comparison of Actinomyces naeslundii with known probiotics |
| title | Genome comparison of Actinomyces naeslundii with known probiotics |
| title_full | Genome comparison of Actinomyces naeslundii with known probiotics |
| title_fullStr | Genome comparison of Actinomyces naeslundii with known probiotics |
| title_full_unstemmed | Genome comparison of Actinomyces naeslundii with known probiotics |
| title_short | Genome comparison of Actinomyces naeslundii with known probiotics |
| title_sort | genome comparison of actinomyces naeslundii with known probiotics |
| topic | QR Microbiology RK Dentistry |
| url | http://irep.iium.edu.my/80870/ http://irep.iium.edu.my/80870/ http://irep.iium.edu.my/80870/1/637-Manuscript-1800-1-10-20200116.pdf |