Isolation and preliminary screening of endophytic fungi from Ficus carica for biocontrol and phosphate solubilization

Fungal endophytes are a special group of symbionts that invade the internal environment of the host organism without causing any pathogenic symptoms. They increase the vigor of the plant while protecting them from several biotic and abiotic stress conditions such as herbivory, disease, and drought....

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Main Authors: Mohd Rosli, Norsalsabila, Abu Hasan Ashari, Khairul Izzah, Ahmad Azmi, Nur Sabrina
Format: Article
Language:English
Published: Zibeline International Publishing 2020
Subjects:
Online Access:http://irep.iium.edu.my/80863/
http://irep.iium.edu.my/80863/1/80863_Isolation%20and%20preliminary%20screening_article.pdf
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author Mohd Rosli, Norsalsabila
Abu Hasan Ashari, Khairul Izzah
Ahmad Azmi, Nur Sabrina
author_facet Mohd Rosli, Norsalsabila
Abu Hasan Ashari, Khairul Izzah
Ahmad Azmi, Nur Sabrina
author_sort Mohd Rosli, Norsalsabila
building IIUM Repository
collection Online Access
description Fungal endophytes are a special group of symbionts that invade the internal environment of the host organism without causing any pathogenic symptoms. They increase the vigor of the plant while protecting them from several biotic and abiotic stress conditions such as herbivory, disease, and drought. In this study, endophytic fungi were isolated from fig (Ficus carica), a beneficial plant belongs to the family of Moraceae. This study was conducted to determine the potential of fungal endophytes of F.carica as biocontrol agents against selected phytopathogens Ganoderma boninense, Magnaporthe oryzae, and Fusarium verticillioides. Besides, endophytic fungi isolates were also screened to assess their ability as a phosphate-solubilizing agent. Results obtained revealed that a total of 11 fungal endophytes was successfully isolated from leaf, root, and stem samples of F.carica. The cultural-morphological identifications were carried out on the unknown fungal isolates. For the antagonistic test, S2-1 and R3-4 show a promising potential against those phytopathogens with more than 20% Percentage Inhibition Radial Growth (PIRG). Endophyte S2-1 exhibits the competition mechanism, while R3-4 expresses the antibiosis mechanism in suppressing the mycelium growth of phytopathogens. As for phosphate solubilization, 8 of 10 isolates show positive results as phosphate solubilizer with the highest PSI value 3.02±0.05 (S2-4).
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institution International Islamic University Malaysia
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spelling iium-808632020-09-25T07:48:08Z http://irep.iium.edu.my/80863/ Isolation and preliminary screening of endophytic fungi from Ficus carica for biocontrol and phosphate solubilization Mohd Rosli, Norsalsabila Abu Hasan Ashari, Khairul Izzah Ahmad Azmi, Nur Sabrina S Agriculture (General) Fungal endophytes are a special group of symbionts that invade the internal environment of the host organism without causing any pathogenic symptoms. They increase the vigor of the plant while protecting them from several biotic and abiotic stress conditions such as herbivory, disease, and drought. In this study, endophytic fungi were isolated from fig (Ficus carica), a beneficial plant belongs to the family of Moraceae. This study was conducted to determine the potential of fungal endophytes of F.carica as biocontrol agents against selected phytopathogens Ganoderma boninense, Magnaporthe oryzae, and Fusarium verticillioides. Besides, endophytic fungi isolates were also screened to assess their ability as a phosphate-solubilizing agent. Results obtained revealed that a total of 11 fungal endophytes was successfully isolated from leaf, root, and stem samples of F.carica. The cultural-morphological identifications were carried out on the unknown fungal isolates. For the antagonistic test, S2-1 and R3-4 show a promising potential against those phytopathogens with more than 20% Percentage Inhibition Radial Growth (PIRG). Endophyte S2-1 exhibits the competition mechanism, while R3-4 expresses the antibiosis mechanism in suppressing the mycelium growth of phytopathogens. As for phosphate solubilization, 8 of 10 isolates show positive results as phosphate solubilizer with the highest PSI value 3.02±0.05 (S2-4). Zibeline International Publishing 2020-06-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/80863/1/80863_Isolation%20and%20preliminary%20screening_article.pdf Mohd Rosli, Norsalsabila and Abu Hasan Ashari, Khairul Izzah and Ahmad Azmi, Nur Sabrina (2020) Isolation and preliminary screening of endophytic fungi from Ficus carica for biocontrol and phosphate solubilization. Environment and Ecosystem Science, 4 (2). pp. 65-72. ISSN 2521-0882 E-ISSN 2521-0483 https://environecosystem.com/ees-02-2020-65-72/ 10.26480/ees.02.2020.65.72
spellingShingle S Agriculture (General)
Mohd Rosli, Norsalsabila
Abu Hasan Ashari, Khairul Izzah
Ahmad Azmi, Nur Sabrina
Isolation and preliminary screening of endophytic fungi from Ficus carica for biocontrol and phosphate solubilization
title Isolation and preliminary screening of endophytic fungi from Ficus carica for biocontrol and phosphate solubilization
title_full Isolation and preliminary screening of endophytic fungi from Ficus carica for biocontrol and phosphate solubilization
title_fullStr Isolation and preliminary screening of endophytic fungi from Ficus carica for biocontrol and phosphate solubilization
title_full_unstemmed Isolation and preliminary screening of endophytic fungi from Ficus carica for biocontrol and phosphate solubilization
title_short Isolation and preliminary screening of endophytic fungi from Ficus carica for biocontrol and phosphate solubilization
title_sort isolation and preliminary screening of endophytic fungi from ficus carica for biocontrol and phosphate solubilization
topic S Agriculture (General)
url http://irep.iium.edu.my/80863/
http://irep.iium.edu.my/80863/
http://irep.iium.edu.my/80863/
http://irep.iium.edu.my/80863/1/80863_Isolation%20and%20preliminary%20screening_article.pdf