Membrane retention potential of Tachypleus gigas during early embryogenesis
Investigation on metal ion (Cd2+ and Pb2+) retention within the embryonic shell of Tachypleus gigas embryos was investigated. Inductively coupled plasma optical emission spectrometry was used for metal observations whereas developmental abnormalities were observed using a scanning electron micros...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Desalination Publications
2020
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/80800/ http://irep.iium.edu.my/80800/1/80800_Membrane%20retention%20potential%20of%20Tachypleus.pdf http://irep.iium.edu.my/80800/7/80800_Membrane%20retention%20potential%20of%20Tachypleus%20gigas_SCOPUS.pdf |
| Summary: | Investigation on metal ion (Cd2+ and Pb2+) retention within the embryonic shell of Tachypleus gigas
embryos was investigated. Inductively coupled plasma optical emission spectrometry was used for
metal observations whereas developmental abnormalities were observed using a scanning electron
microscope and histology via Harrison-Eosin staining. Metal ions penetrate and regulate between the
extra- and embryonic shell environments to cause delayed embryogenesis only in early embryonic
stages (Stages-E and DE). Ionic regulation was studied and findings showed that the embryonic shell
(ES) was capable of selecting required ions whereas the extra-embryonic shell (EES) having higher
retention potential. Throughout the 34 d of exposure, Se2+ was accumulated in the yolk mass (YM).
Ionic regulation of embryos was influenced by stocking density and concentration of metabolites.
Bioaccumulation factor revealed accumulation potential as EES > ES > YM and thus EES is suitable
for biological membrane development. The mechanism of ionic regulation in the ES membrane can be
used as a model to design a biological membrane capable of removing metals from polluted waters. |
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