Assessment of free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from Tetracera macrophylla leaves
Tetracera macrophylla is a valuable medicinal plant used traditionally for the treatment of various ailments. The crude ethanol extract was analyzed for its total phenolic and flavonoid contents. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-3-ethylbenzothiazine-6-sulfonic acid (ABTS) were...
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Elsevier GmbH
2020
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| Online Access: | http://irep.iium.edu.my/80752/ http://irep.iium.edu.my/80752/19/80752_Assessment%20of%20Free%20radical%20scavenging%20and%20digestive_article_new.pdf http://irep.iium.edu.my/80752/13/80752_Assessment%20of%20Free%20radical%20scavenging%20and%20digestive%20enzyme%20inhibitory_SCOPUS.pdf http://irep.iium.edu.my/80752/20/80752_Assessment%20of%20Free%20radical%20scavenging%20and%20digestive_wos.pdf |
| _version_ | 1848789015382196224 |
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| author | Opeyemi Roheem, Fatimah Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Ali Shah, Syed Adnan Latip, Jalifah Alhassan, Alhassan Muhammad Syed Mohammad, Sharifah Nurul Akilah |
| author_facet | Opeyemi Roheem, Fatimah Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Ali Shah, Syed Adnan Latip, Jalifah Alhassan, Alhassan Muhammad Syed Mohammad, Sharifah Nurul Akilah |
| author_sort | Opeyemi Roheem, Fatimah |
| building | IIUM Repository |
| collection | Online Access |
| description | Tetracera macrophylla is a valuable medicinal plant used traditionally for the treatment of various ailments. The crude ethanol extract was analyzed for its total phenolic and flavonoid contents. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-3-ethylbenzothiazine-6-sulfonic acid (ABTS) were used to examine the free radical scavenging capacity while enzyme inhibitory activity was assessed using α-amylase and α-glucosidase inhibitory methods. The crude extract was found to be rich in both phenolic and flavonoid contents (417.90 and5.86 μg GAE and QUE/mg dry weight, respectively) thus exhibited good radical scavenging activity on DPPH and ABTS radicals (IC50 values of 16.2 and 14.65 μg/mL, respectively). It also showed a promising concentration dependent enzymes inhibitory activity against α-amylase and α-glucosidase with IC50 of 72.17 and 76.16 μg/mL, respectively. Fractionation of active ethanol extract of T. macrophylla leaves through liquid-liquid partitioning yielded fractions Fa, Fb, Fc, and Fd. Among all the fractions, Fb (ethyl acetate fraction) showed the highest phenolic and flavonoid contents (206.34 μg/ GAE and 4.01 μg/ QUE respectively) and was also found to exhibit highest antioxidant and α-glucosidase inhibitory activities. The most active fraction, Fb upon purification yielded five compounds which were characterized and identified through different spectroscopic techniques. FTQ-4 among the compounds exhibited the strongest free radical and enzyme inhibitory activities followed by FTQ-3. All the compounds were isolated from this plant for the first time and found active against free radicals and/or digestive enzymes.These results further suggest the medicinal potential of T. macrophylla leaves as a therapeutic agent against hyperglycemia and free radical associated problems. |
| first_indexed | 2025-11-14T17:50:00Z |
| format | Article |
| id | iium-80752 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:50:00Z |
| publishDate | 2020 |
| publisher | Elsevier GmbH |
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| spelling | iium-807522021-05-06T03:43:42Z http://irep.iium.edu.my/80752/ Assessment of free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from Tetracera macrophylla leaves Opeyemi Roheem, Fatimah Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Ali Shah, Syed Adnan Latip, Jalifah Alhassan, Alhassan Muhammad Syed Mohammad, Sharifah Nurul Akilah QD Chemistry Tetracera macrophylla is a valuable medicinal plant used traditionally for the treatment of various ailments. The crude ethanol extract was analyzed for its total phenolic and flavonoid contents. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-3-ethylbenzothiazine-6-sulfonic acid (ABTS) were used to examine the free radical scavenging capacity while enzyme inhibitory activity was assessed using α-amylase and α-glucosidase inhibitory methods. The crude extract was found to be rich in both phenolic and flavonoid contents (417.90 and5.86 μg GAE and QUE/mg dry weight, respectively) thus exhibited good radical scavenging activity on DPPH and ABTS radicals (IC50 values of 16.2 and 14.65 μg/mL, respectively). It also showed a promising concentration dependent enzymes inhibitory activity against α-amylase and α-glucosidase with IC50 of 72.17 and 76.16 μg/mL, respectively. Fractionation of active ethanol extract of T. macrophylla leaves through liquid-liquid partitioning yielded fractions Fa, Fb, Fc, and Fd. Among all the fractions, Fb (ethyl acetate fraction) showed the highest phenolic and flavonoid contents (206.34 μg/ GAE and 4.01 μg/ QUE respectively) and was also found to exhibit highest antioxidant and α-glucosidase inhibitory activities. The most active fraction, Fb upon purification yielded five compounds which were characterized and identified through different spectroscopic techniques. FTQ-4 among the compounds exhibited the strongest free radical and enzyme inhibitory activities followed by FTQ-3. All the compounds were isolated from this plant for the first time and found active against free radicals and/or digestive enzymes.These results further suggest the medicinal potential of T. macrophylla leaves as a therapeutic agent against hyperglycemia and free radical associated problems. Elsevier GmbH 2020-04-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/80752/19/80752_Assessment%20of%20Free%20radical%20scavenging%20and%20digestive_article_new.pdf application/pdf en http://irep.iium.edu.my/80752/13/80752_Assessment%20of%20Free%20radical%20scavenging%20and%20digestive%20enzyme%20inhibitory_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/80752/20/80752_Assessment%20of%20Free%20radical%20scavenging%20and%20digestive_wos.pdf Opeyemi Roheem, Fatimah and Ahmed, Qamar Uddin and Mat So'ad, Siti Zaiton and Ali Shah, Syed Adnan and Latip, Jalifah and Alhassan, Alhassan Muhammad and Syed Mohammad, Sharifah Nurul Akilah (2020) Assessment of free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from Tetracera macrophylla leaves. Journal of Herbal Medicine, 22. pp. 1-8. ISSN 2210-8033 https://www.sciencedirect.com/science/article/abs/pii/S2210803320300233 10.1016/j.hermed.2020.100351 |
| spellingShingle | QD Chemistry Opeyemi Roheem, Fatimah Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Ali Shah, Syed Adnan Latip, Jalifah Alhassan, Alhassan Muhammad Syed Mohammad, Sharifah Nurul Akilah Assessment of free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from Tetracera macrophylla leaves |
| title | Assessment of free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from Tetracera macrophylla leaves |
| title_full | Assessment of free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from Tetracera macrophylla leaves |
| title_fullStr | Assessment of free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from Tetracera macrophylla leaves |
| title_full_unstemmed | Assessment of free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from Tetracera macrophylla leaves |
| title_short | Assessment of free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from Tetracera macrophylla leaves |
| title_sort | assessment of free radical scavenging and digestive enzyme inhibitory activities of extract, fractions and isolated compounds from tetracera macrophylla leaves |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/80752/ http://irep.iium.edu.my/80752/ http://irep.iium.edu.my/80752/ http://irep.iium.edu.my/80752/19/80752_Assessment%20of%20Free%20radical%20scavenging%20and%20digestive_article_new.pdf http://irep.iium.edu.my/80752/13/80752_Assessment%20of%20Free%20radical%20scavenging%20and%20digestive%20enzyme%20inhibitory_SCOPUS.pdf http://irep.iium.edu.my/80752/20/80752_Assessment%20of%20Free%20radical%20scavenging%20and%20digestive_wos.pdf |