Anti-inflammatory effects of trihoney in hypercholesterolemic atherosclerotic rabbits: a comparative study with atorvastatin
Introduction: Hypercholesterolemia has been proven as a main pathogenic trigger for pathogenesis of atherosclero- sis. Atherosclerosis characterised by chronic inflammatory process and increased expression of inflammatory mark- ers. In this study; Trihoney (a combination of three types of natural ho...
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Universiti Putra Malaysia
2020
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| Online Access: | http://irep.iium.edu.my/80335/ http://irep.iium.edu.my/80335/1/80335_Anti-inflammatory%20Effects%20of%20Trihoney.pdf http://irep.iium.edu.my/80335/7/80335_Anti-inflammatory%20effects%20of%20trihoney%20in%20hypercholesterolemic_scopus.pdf |
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| author | Hamad Alfarisi, Hamad Abdulsalam Ibrahim, Muhammad Azahari, Nuraniza Hamad Mohamed, Zenab Hamdan, Asmah Hanim Che Mohamad, Che Anuar |
| author_facet | Hamad Alfarisi, Hamad Abdulsalam Ibrahim, Muhammad Azahari, Nuraniza Hamad Mohamed, Zenab Hamdan, Asmah Hanim Che Mohamad, Che Anuar |
| author_sort | Hamad Alfarisi, Hamad Abdulsalam |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Hypercholesterolemia has been proven as a main pathogenic trigger for pathogenesis of atherosclero- sis. Atherosclerosis characterised by chronic inflammatory process and increased expression of inflammatory mark- ers. In this study; Trihoney (a combination of three types of natural honey namely: Trigona, mellifera, and Dorsata) was investigated for its anti-inflammatory effect in hypercholesterolemic atherosclerotic rabbits. Methods: Thirty male New Zealand white rabbits (NZW) were grouped into: normal diet (C), normal diet with 0.6g/kg/day of Tri- honey (C+H), 1% cholesterol diet (HCD), 1% cholesterol diet with 0.6g/kg/day of Trihoney (HCD+H), and 1% cholesterol diet with 2mg/kg/day of atorvastatin (HCD+At.). After 12 weeks of starting the experiment, animals were sacrificed and serum analysed for homocysteine and pro-atherogenic inflammatory markers such as: interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumour necrosis factor-α (TNF-α). Fasting serum glucose was analysed to assess gly- caemic status. Results: Trihoney treated group showed significantly lower (p<0.05) serum IL-1β and IL-6 compared to the HCD group. Trihoney supplementation resulted in significant (p<0.001) reduction of serum TNF-α compared to HCD group. Experimental group HCD had serum homocysteine level comparable to that of the control groups with- out any significant difference despite little increase in the mean value. Trihoney treated group had serum homocys- teine comparable to the controls. All experimental groups showed fasting serum glucose comparable to the control. Conclusion: This study showed that Trihoney has an anti-inflammatory function and may be used as an adjuvant to statins for management of atherosclerotic cardiovascular diseases even in diabetic subjects.
Keywords: Atherosclerosis, Atorvastatin, Inflammatory markers, Trihoney |
| first_indexed | 2025-11-14T17:48:47Z |
| format | Article |
| id | iium-80335 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:48:47Z |
| publishDate | 2020 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
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| spelling | iium-803352023-11-21T01:29:41Z http://irep.iium.edu.my/80335/ Anti-inflammatory effects of trihoney in hypercholesterolemic atherosclerotic rabbits: a comparative study with atorvastatin Hamad Alfarisi, Hamad Abdulsalam Ibrahim, Muhammad Azahari, Nuraniza Hamad Mohamed, Zenab Hamdan, Asmah Hanim Che Mohamad, Che Anuar Q128 Islam and Science Q300 Cybernetics Introduction: Hypercholesterolemia has been proven as a main pathogenic trigger for pathogenesis of atherosclero- sis. Atherosclerosis characterised by chronic inflammatory process and increased expression of inflammatory mark- ers. In this study; Trihoney (a combination of three types of natural honey namely: Trigona, mellifera, and Dorsata) was investigated for its anti-inflammatory effect in hypercholesterolemic atherosclerotic rabbits. Methods: Thirty male New Zealand white rabbits (NZW) were grouped into: normal diet (C), normal diet with 0.6g/kg/day of Tri- honey (C+H), 1% cholesterol diet (HCD), 1% cholesterol diet with 0.6g/kg/day of Trihoney (HCD+H), and 1% cholesterol diet with 2mg/kg/day of atorvastatin (HCD+At.). After 12 weeks of starting the experiment, animals were sacrificed and serum analysed for homocysteine and pro-atherogenic inflammatory markers such as: interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumour necrosis factor-α (TNF-α). Fasting serum glucose was analysed to assess gly- caemic status. Results: Trihoney treated group showed significantly lower (p<0.05) serum IL-1β and IL-6 compared to the HCD group. Trihoney supplementation resulted in significant (p<0.001) reduction of serum TNF-α compared to HCD group. Experimental group HCD had serum homocysteine level comparable to that of the control groups with- out any significant difference despite little increase in the mean value. Trihoney treated group had serum homocys- teine comparable to the controls. All experimental groups showed fasting serum glucose comparable to the control. Conclusion: This study showed that Trihoney has an anti-inflammatory function and may be used as an adjuvant to statins for management of atherosclerotic cardiovascular diseases even in diabetic subjects. Keywords: Atherosclerosis, Atorvastatin, Inflammatory markers, Trihoney Universiti Putra Malaysia 2020-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/80335/1/80335_Anti-inflammatory%20Effects%20of%20Trihoney.pdf application/pdf en http://irep.iium.edu.my/80335/7/80335_Anti-inflammatory%20effects%20of%20trihoney%20in%20hypercholesterolemic_scopus.pdf Hamad Alfarisi, Hamad Abdulsalam and Ibrahim, Muhammad and Azahari, Nuraniza and Hamad Mohamed, Zenab and Hamdan, Asmah Hanim and Che Mohamad, Che Anuar (2020) Anti-inflammatory effects of trihoney in hypercholesterolemic atherosclerotic rabbits: a comparative study with atorvastatin. Malaysian Journal of Medicine and Health Sciences, 16 (2). pp. 230-236. ISSN 1675-8544 E-ISSN 2636-9346 https://medic.upm.edu.my/upload/dokumen/2020042010341232_MJMHS_0591.pdf |
| spellingShingle | Q128 Islam and Science Q300 Cybernetics Hamad Alfarisi, Hamad Abdulsalam Ibrahim, Muhammad Azahari, Nuraniza Hamad Mohamed, Zenab Hamdan, Asmah Hanim Che Mohamad, Che Anuar Anti-inflammatory effects of trihoney in hypercholesterolemic atherosclerotic rabbits: a comparative study with atorvastatin |
| title | Anti-inflammatory effects of trihoney in hypercholesterolemic
atherosclerotic rabbits: a comparative study with atorvastatin |
| title_full | Anti-inflammatory effects of trihoney in hypercholesterolemic
atherosclerotic rabbits: a comparative study with atorvastatin |
| title_fullStr | Anti-inflammatory effects of trihoney in hypercholesterolemic
atherosclerotic rabbits: a comparative study with atorvastatin |
| title_full_unstemmed | Anti-inflammatory effects of trihoney in hypercholesterolemic
atherosclerotic rabbits: a comparative study with atorvastatin |
| title_short | Anti-inflammatory effects of trihoney in hypercholesterolemic
atherosclerotic rabbits: a comparative study with atorvastatin |
| title_sort | anti-inflammatory effects of trihoney in hypercholesterolemic
atherosclerotic rabbits: a comparative study with atorvastatin |
| topic | Q128 Islam and Science Q300 Cybernetics |
| url | http://irep.iium.edu.my/80335/ http://irep.iium.edu.my/80335/ http://irep.iium.edu.my/80335/1/80335_Anti-inflammatory%20Effects%20of%20Trihoney.pdf http://irep.iium.edu.my/80335/7/80335_Anti-inflammatory%20effects%20of%20trihoney%20in%20hypercholesterolemic_scopus.pdf |