Supplements of an aqueous combination of Justicia adhatoda and Ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemia

Background: Justicia adhatoda and Ocimum tenuiflorum, locally known as bashok and tulsi, are two ethnomedicinally important herbs that have been using as dietary supplements for several therapeutic applications. This study evaluated the combined effect of both the herbs as an antioxidative and antih...

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Main Authors: Chowdhury, Ifrahim Iqbal, Rahman, Md Atiar, A. Hashem, Mohammad, Hossain Bhuiyan, M. Mosharef, Hajjar, Dina, Alelwani, Walla, A. Makki, Arwa, Haque, Md. Areeful, Tangpong, Jitbanjong, Bakhtiar, M. Taher
Format: Article
Language:English
Published: 2020
Subjects:
Online Access:http://irep.iium.edu.my/80303/
http://irep.iium.edu.my/80303/1/ame2.12115.pdf
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author Chowdhury, Ifrahim Iqbal
Rahman, Md Atiar
A. Hashem, Mohammad
Hossain Bhuiyan, M. Mosharef
Hajjar, Dina
Alelwani, Walla
A. Makki, Arwa
Haque, Md. Areeful
Tangpong, Jitbanjong
Bakhtiar, M. Taher
author_facet Chowdhury, Ifrahim Iqbal
Rahman, Md Atiar
A. Hashem, Mohammad
Hossain Bhuiyan, M. Mosharef
Hajjar, Dina
Alelwani, Walla
A. Makki, Arwa
Haque, Md. Areeful
Tangpong, Jitbanjong
Bakhtiar, M. Taher
author_sort Chowdhury, Ifrahim Iqbal
building IIUM Repository
collection Online Access
description Background: Justicia adhatoda and Ocimum tenuiflorum, locally known as bashok and tulsi, are two ethnomedicinally important herbs that have been using as dietary supplements for several therapeutic applications. This study evaluated the combined effect of both the herbs as an antioxidative and antihyperlipidemic agent. Methods: Antihyperlipidemic effect was assessed in a high-fat diet-induced hyperlipidemic model in Wistar albino rats. The rats were treated orally with extracts of bashok (J adhatoda, 200 mg/kg bw), tulshi (O tenuiflorum, 200 mg/kg bw), and a combination of bashok and tulshi (50:50), as well as with a reference drug, atorvastatin (10 mg/kg/day), with or without high-fat diet for 14 days. The antioxidative effect was studied using established in vitro models. The studies were supported by experimentally testing the effects of the extracts on membrane stabilization and inhibition of protein denaturation. Results: The results showed that the serum lipid profile was significantly decreased in the different treatment groups, with bashok having the greatest effect. Body weights, total serum protein, LDH, and relative liver and adipose tissue weights were markedly restored towards baseline values, the lowest atherogenic index being achieved with the combined extract. The combination treatment significantly enhanced total phenolic content and antioxidative capacity and greatly potentiated membrane stabilization, but inhibition of protein denaturation was not significantly affected. Conclusion: The data demonstrate that a combination of Justicia adhatoda and Ocimum tenuiflorum could be developed as a food supplement with antioxidative and antihyperlipidemic benefits.
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spelling iium-803032020-05-01T05:00:24Z http://irep.iium.edu.my/80303/ Supplements of an aqueous combination of Justicia adhatoda and Ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemia Chowdhury, Ifrahim Iqbal Rahman, Md Atiar A. Hashem, Mohammad Hossain Bhuiyan, M. Mosharef Hajjar, Dina Alelwani, Walla A. Makki, Arwa Haque, Md. Areeful Tangpong, Jitbanjong Bakhtiar, M. Taher RS Pharmacy and materia medica Background: Justicia adhatoda and Ocimum tenuiflorum, locally known as bashok and tulsi, are two ethnomedicinally important herbs that have been using as dietary supplements for several therapeutic applications. This study evaluated the combined effect of both the herbs as an antioxidative and antihyperlipidemic agent. Methods: Antihyperlipidemic effect was assessed in a high-fat diet-induced hyperlipidemic model in Wistar albino rats. The rats were treated orally with extracts of bashok (J adhatoda, 200 mg/kg bw), tulshi (O tenuiflorum, 200 mg/kg bw), and a combination of bashok and tulshi (50:50), as well as with a reference drug, atorvastatin (10 mg/kg/day), with or without high-fat diet for 14 days. The antioxidative effect was studied using established in vitro models. The studies were supported by experimentally testing the effects of the extracts on membrane stabilization and inhibition of protein denaturation. Results: The results showed that the serum lipid profile was significantly decreased in the different treatment groups, with bashok having the greatest effect. Body weights, total serum protein, LDH, and relative liver and adipose tissue weights were markedly restored towards baseline values, the lowest atherogenic index being achieved with the combined extract. The combination treatment significantly enhanced total phenolic content and antioxidative capacity and greatly potentiated membrane stabilization, but inhibition of protein denaturation was not significantly affected. Conclusion: The data demonstrate that a combination of Justicia adhatoda and Ocimum tenuiflorum could be developed as a food supplement with antioxidative and antihyperlipidemic benefits. 2020-04-30 Article PeerReviewed application/pdf en http://irep.iium.edu.my/80303/1/ame2.12115.pdf Chowdhury, Ifrahim Iqbal and Rahman, Md Atiar and A. Hashem, Mohammad and Hossain Bhuiyan, M. Mosharef and Hajjar, Dina and Alelwani, Walla and A. Makki, Arwa and Haque, Md. Areeful and Tangpong, Jitbanjong and Bakhtiar, M. Taher (2020) Supplements of an aqueous combination of Justicia adhatoda and Ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemia. Animal Models and Experimental Medicine. pp. 1-12. ISSN 2576-2095 (In Press) https://onlinelibrary.wiley.com/doi/10.1002/ame2.12115 https://doi.org/10.1002/ame2.12115
spellingShingle RS Pharmacy and materia medica
Chowdhury, Ifrahim Iqbal
Rahman, Md Atiar
A. Hashem, Mohammad
Hossain Bhuiyan, M. Mosharef
Hajjar, Dina
Alelwani, Walla
A. Makki, Arwa
Haque, Md. Areeful
Tangpong, Jitbanjong
Bakhtiar, M. Taher
Supplements of an aqueous combination of Justicia adhatoda and Ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemia
title Supplements of an aqueous combination of Justicia adhatoda and Ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemia
title_full Supplements of an aqueous combination of Justicia adhatoda and Ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemia
title_fullStr Supplements of an aqueous combination of Justicia adhatoda and Ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemia
title_full_unstemmed Supplements of an aqueous combination of Justicia adhatoda and Ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemia
title_short Supplements of an aqueous combination of Justicia adhatoda and Ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemia
title_sort supplements of an aqueous combination of justicia adhatoda and ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemia
topic RS Pharmacy and materia medica
url http://irep.iium.edu.my/80303/
http://irep.iium.edu.my/80303/
http://irep.iium.edu.my/80303/
http://irep.iium.edu.my/80303/1/ame2.12115.pdf