Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study
Introduction: Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Humans are exposed to arsenic through drinking water and anthropogenic activities. Exposure to inorganic arsenic has been linked with multiple health problems. However, studies focusing on c...
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| Language: | English |
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Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
2019
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| Online Access: | http://irep.iium.edu.my/80024/ http://irep.iium.edu.my/80024/1/616-Manuscript-1764-1-10-20200115.pdf |
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| author | Wan Salleh, Wan Muhamad Salahudin A. Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah |
| author_facet | Wan Salleh, Wan Muhamad Salahudin A. Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah |
| author_sort | Wan Salleh, Wan Muhamad Salahudin |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Humans are exposed to arsenic through drinking water and anthropogenic activities. Exposure to inorganic arsenic has been linked with multiple health problems. However, studies focusing on chronic organic arsenic exposure and its adverse effects on kidney were limited. The purpose of current study was to determine the effects of chronic organic arsenic exposure in rats kidney by light and electron microscopy. Materials and Method: Thirty-six male SpragueDawley rats were divided into six groups (n=6); three control and three treatment group respectively. All the control group was given distilled water via oral gavage. The treatment group was given oral gavage of MSMA at 42.10 mg/kg body weight (BW) which is equivalent 1/30 LD50 of MSMA. The control and treatment groups were sacrificed at two month, four month and six months interval. Both kidneys harvested for light microscopy and electron microscopy study. Results: Showed progressive changes. The changes initially focal and became diffused involving glomerular; such as glomerular hypercellularity, glomerular shrinkage and dilated Bowman's space. Meanwhile, in proximal tubules, showed diminished brush borders, detachment of nucleus and basement membrane thickening. Electron microscopy showed flattened cell bodies of podocytes, effacement and fusion of podocytes foot processes, thickening of glomerular basement membrane, and discontinuity of brush border. The control and two-months treated group appeared to be normal. Conclusion: Chronic organic arsenic (MSMA) exposure induced chronic kidney injury. |
| first_indexed | 2025-11-14T17:48:01Z |
| format | Article |
| id | iium-80024 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:48:01Z |
| publishDate | 2019 |
| publisher | Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-800242020-04-09T06:27:53Z http://irep.iium.edu.my/80024/ Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study Wan Salleh, Wan Muhamad Salahudin A. Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah RB Pathology Introduction: Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Humans are exposed to arsenic through drinking water and anthropogenic activities. Exposure to inorganic arsenic has been linked with multiple health problems. However, studies focusing on chronic organic arsenic exposure and its adverse effects on kidney were limited. The purpose of current study was to determine the effects of chronic organic arsenic exposure in rats kidney by light and electron microscopy. Materials and Method: Thirty-six male SpragueDawley rats were divided into six groups (n=6); three control and three treatment group respectively. All the control group was given distilled water via oral gavage. The treatment group was given oral gavage of MSMA at 42.10 mg/kg body weight (BW) which is equivalent 1/30 LD50 of MSMA. The control and treatment groups were sacrificed at two month, four month and six months interval. Both kidneys harvested for light microscopy and electron microscopy study. Results: Showed progressive changes. The changes initially focal and became diffused involving glomerular; such as glomerular hypercellularity, glomerular shrinkage and dilated Bowman's space. Meanwhile, in proximal tubules, showed diminished brush borders, detachment of nucleus and basement membrane thickening. Electron microscopy showed flattened cell bodies of podocytes, effacement and fusion of podocytes foot processes, thickening of glomerular basement membrane, and discontinuity of brush border. The control and two-months treated group appeared to be normal. Conclusion: Chronic organic arsenic (MSMA) exposure induced chronic kidney injury. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2019-12-31 Article PeerReviewed application/pdf en http://irep.iium.edu.my/80024/1/616-Manuscript-1764-1-10-20200115.pdf Wan Salleh, Wan Muhamad Salahudin and A. Talib, Norlelawati and Abdullah, Nor Zamzila and Aung, Sanda and Buyong, Zunariah (2019) Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study. IIUM Medical Journal Malaysia (IMJM), 18 (2 (Supplementary)). p. 41. E-ISSN 1823-4631 https://journals.iium.edu.my/kom/index.php/imjm/article/view/616 |
| spellingShingle | RB Pathology Wan Salleh, Wan Muhamad Salahudin A. Talib, Norlelawati Abdullah, Nor Zamzila Aung, Sanda Buyong, Zunariah Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study |
| title | Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study |
| title_full | Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study |
| title_fullStr | Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study |
| title_full_unstemmed | Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study |
| title_short | Effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study |
| title_sort | effects of chronic organic arsenic exposure on rats kidney - light microscopy and electron microscopy study |
| topic | RB Pathology |
| url | http://irep.iium.edu.my/80024/ http://irep.iium.edu.my/80024/ http://irep.iium.edu.my/80024/1/616-Manuscript-1764-1-10-20200115.pdf |