Chronic low dose organic arsenic induced liver structural damage

Introduction: Over the decades, organic arsenic has been thought to be less toxic than inorganic arsenic. Monosodium methylarsonate (MSMA) is a potent organoarsenical herbicide that is still being used in most Asian countries. Reported studies on the effects of organic arsenic are mainly to the gast...

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Main Authors: Saharudin, Shahida, Abdullah, Nor Zamzila, A. Talib, Norlelawati, Ab Rahman, Jamalludin, Abd. Fuaat, Azliana, Buyong, Zunariah
Format: Article
Language:English
English
Published: IIUM Press 2020
Subjects:
Online Access:http://irep.iium.edu.my/80022/
http://irep.iium.edu.my/80022/1/IMJM%20Vol%2019%20pg%2037-44%20Chronic%20Low%20Dose%20Organic%20Arsenic%20%26%20Liver.pdf
http://irep.iium.edu.my/80022/7/80022_Chronic%20low%20dose%20organic%20arsenic%20induced_SCOPUS.pdf
_version_ 1848788889860308992
author Saharudin, Shahida
Abdullah, Nor Zamzila
A. Talib, Norlelawati
Ab Rahman, Jamalludin
Abd. Fuaat, Azliana
Buyong, Zunariah
author_facet Saharudin, Shahida
Abdullah, Nor Zamzila
A. Talib, Norlelawati
Ab Rahman, Jamalludin
Abd. Fuaat, Azliana
Buyong, Zunariah
author_sort Saharudin, Shahida
building IIUM Repository
collection Online Access
description Introduction: Over the decades, organic arsenic has been thought to be less toxic than inorganic arsenic. Monosodium methylarsonate (MSMA) is a potent organoarsenical herbicide that is still being used in most Asian countries. Reported studies on the effects of organic arsenic are mainly to the gastrointestinal system, however there are limited research on its impacts to the liver. Therefore, this study aimed to investigate the effect of MSMA exposure on hepatocytes and liver sinusoidal endothelial cells (LSEC). Materials and Methods: Fourteen Sprague Dawley rats (n=14) were divided equally into arsenic-exposed (n=7) and control (n=7) groups. The rats in arsenic-exposed group were given MSMA at 63.20 mg/kg daily for 6 months through oral gavage. While the rats in control group were given distilled water ad libitum. At the end of the duration, they were euthanized and underwent liver perfusion for tissue preservation. Liver tissues were harvested and processed for light microscopy, scanning and transmission electron microscopy. The findings were analysed descriptively. Results: MSMA had caused necrotic and apoptotic changes to the liver. Normal organelles morphology were loss in the hepatocytes while LSEC revealed defenestration. Conclusion: In this study, chronic low dose organic arsenic exposure showed evidence of toxicity to hepatocytes. Interestingly, LSEC demonstrated capillarization changes.
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publishDate 2020
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spelling iium-800222020-11-18T08:55:31Z http://irep.iium.edu.my/80022/ Chronic low dose organic arsenic induced liver structural damage Saharudin, Shahida Abdullah, Nor Zamzila A. Talib, Norlelawati Ab Rahman, Jamalludin Abd. Fuaat, Azliana Buyong, Zunariah RB Pathology Introduction: Over the decades, organic arsenic has been thought to be less toxic than inorganic arsenic. Monosodium methylarsonate (MSMA) is a potent organoarsenical herbicide that is still being used in most Asian countries. Reported studies on the effects of organic arsenic are mainly to the gastrointestinal system, however there are limited research on its impacts to the liver. Therefore, this study aimed to investigate the effect of MSMA exposure on hepatocytes and liver sinusoidal endothelial cells (LSEC). Materials and Methods: Fourteen Sprague Dawley rats (n=14) were divided equally into arsenic-exposed (n=7) and control (n=7) groups. The rats in arsenic-exposed group were given MSMA at 63.20 mg/kg daily for 6 months through oral gavage. While the rats in control group were given distilled water ad libitum. At the end of the duration, they were euthanized and underwent liver perfusion for tissue preservation. Liver tissues were harvested and processed for light microscopy, scanning and transmission electron microscopy. The findings were analysed descriptively. Results: MSMA had caused necrotic and apoptotic changes to the liver. Normal organelles morphology were loss in the hepatocytes while LSEC revealed defenestration. Conclusion: In this study, chronic low dose organic arsenic exposure showed evidence of toxicity to hepatocytes. Interestingly, LSEC demonstrated capillarization changes. IIUM Press 2020-04-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/80022/1/IMJM%20Vol%2019%20pg%2037-44%20Chronic%20Low%20Dose%20Organic%20Arsenic%20%26%20Liver.pdf application/pdf en http://irep.iium.edu.my/80022/7/80022_Chronic%20low%20dose%20organic%20arsenic%20induced_SCOPUS.pdf Saharudin, Shahida and Abdullah, Nor Zamzila and A. Talib, Norlelawati and Ab Rahman, Jamalludin and Abd. Fuaat, Azliana and Buyong, Zunariah (2020) Chronic low dose organic arsenic induced liver structural damage. IIUM Medical Journal Malaysia (IMJM), 19 (1). pp. 37-44. ISSN 1823-4631 https://journals.iium.edu.my/kom/index.php/imjm/article/view/1330
spellingShingle RB Pathology
Saharudin, Shahida
Abdullah, Nor Zamzila
A. Talib, Norlelawati
Ab Rahman, Jamalludin
Abd. Fuaat, Azliana
Buyong, Zunariah
Chronic low dose organic arsenic induced liver structural damage
title Chronic low dose organic arsenic induced liver structural damage
title_full Chronic low dose organic arsenic induced liver structural damage
title_fullStr Chronic low dose organic arsenic induced liver structural damage
title_full_unstemmed Chronic low dose organic arsenic induced liver structural damage
title_short Chronic low dose organic arsenic induced liver structural damage
title_sort chronic low dose organic arsenic induced liver structural damage
topic RB Pathology
url http://irep.iium.edu.my/80022/
http://irep.iium.edu.my/80022/
http://irep.iium.edu.my/80022/1/IMJM%20Vol%2019%20pg%2037-44%20Chronic%20Low%20Dose%20Organic%20Arsenic%20%26%20Liver.pdf
http://irep.iium.edu.my/80022/7/80022_Chronic%20low%20dose%20organic%20arsenic%20induced_SCOPUS.pdf