Screening of macromolecular cross-linkers and food-grade additives for enhancement of catalytic performance of MNP-CLEA-lipase of Hevea brasiliensis
Skim latex from Hevea brasiliensis (rubber tree) consist of many useful proteins and enzymes that can be utilized to produce value added products for industrial purposes. Lipase recovered from skim latex serum was immobilized via cross-linked enzyme aggregates (CLEA) technology, while supported by m...
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| Format: | Proceeding Paper |
| Language: | English English English |
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2020
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| Online Access: | http://irep.iium.edu.my/79841/ http://irep.iium.edu.my/79841/41/79841%20acceptance%20letter%20and%20report.pdf http://irep.iium.edu.my/79841/1/1.%20Amalin%20ICSET%202020%20Paper%20for%20Publication.pdf http://irep.iium.edu.my/79841/7/6.%20Amalin%20ICSET%202020%20Slide%20Presentation.pdf |
| _version_ | 1848788854535880704 |
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| author | Ab Aziz Al Safi, Nur Amalin Yusof, Faridah |
| author_facet | Ab Aziz Al Safi, Nur Amalin Yusof, Faridah |
| author_sort | Ab Aziz Al Safi, Nur Amalin |
| building | IIUM Repository |
| collection | Online Access |
| description | Skim latex from Hevea brasiliensis (rubber tree) consist of many useful proteins and enzymes that can be utilized to produce value added products for industrial purposes. Lipase recovered from skim latex serum was immobilized via cross-linked enzyme aggregates (CLEA) technology, while supported by magnetic nanoparticles for properties enhancement, termed ‘Magnetic Nanoparticles CLEA-lipase’ (MNP-CLEA-lipase). MNP-CLEA-lipase was prepared by chemical cross-linking of enzyme aggregates with amino functionalized magnetic nanoparticles. Instead of using glutaraldehyde as cross-linking agent, green, non-toxic and renewable macromolecular cross-linkers (dextran, chitosan, gum Arabic and pectin) were screened and the best alternative based on highest residual activity was chosen for further analysis. Same goes for additives; the traditional use of expensive BSA was replaced with low cost, food grade additives (egg protein, soy protein and milk protein). The optimum condition parameters such as concentration of cross-linker, concentration of additive and concentration of precipitant were determined using one factor at a time (OFAT) method and structural analysis was done by FTIR. The screening results showed that pectin was the best cross-linker (118 % residual activity) and soy protein was the best additive (129 % residual activity) for MNP-CLEA-lipase with optimum conditions of 180 mg/ml pectin, 0.6 % w/v soy protein and 80 % saturation ammonium sulphate. FTIR of MNP-CLEA-lipase showed an Amide I and Amide V bands which originated from silanized magnetic nanoparticles (MNP) and a new band, Amide II was formed prior to the successful CLEA immobilization of lipase enzyme with MNPs |
| first_indexed | 2025-11-14T17:47:26Z |
| format | Proceeding Paper |
| id | iium-79841 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:47:26Z |
| publishDate | 2020 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-798412020-04-20T07:10:37Z http://irep.iium.edu.my/79841/ Screening of macromolecular cross-linkers and food-grade additives for enhancement of catalytic performance of MNP-CLEA-lipase of Hevea brasiliensis Ab Aziz Al Safi, Nur Amalin Yusof, Faridah T Technology (General) TP155 Chemical engineering TP248.13 Biotechnology Skim latex from Hevea brasiliensis (rubber tree) consist of many useful proteins and enzymes that can be utilized to produce value added products for industrial purposes. Lipase recovered from skim latex serum was immobilized via cross-linked enzyme aggregates (CLEA) technology, while supported by magnetic nanoparticles for properties enhancement, termed ‘Magnetic Nanoparticles CLEA-lipase’ (MNP-CLEA-lipase). MNP-CLEA-lipase was prepared by chemical cross-linking of enzyme aggregates with amino functionalized magnetic nanoparticles. Instead of using glutaraldehyde as cross-linking agent, green, non-toxic and renewable macromolecular cross-linkers (dextran, chitosan, gum Arabic and pectin) were screened and the best alternative based on highest residual activity was chosen for further analysis. Same goes for additives; the traditional use of expensive BSA was replaced with low cost, food grade additives (egg protein, soy protein and milk protein). The optimum condition parameters such as concentration of cross-linker, concentration of additive and concentration of precipitant were determined using one factor at a time (OFAT) method and structural analysis was done by FTIR. The screening results showed that pectin was the best cross-linker (118 % residual activity) and soy protein was the best additive (129 % residual activity) for MNP-CLEA-lipase with optimum conditions of 180 mg/ml pectin, 0.6 % w/v soy protein and 80 % saturation ammonium sulphate. FTIR of MNP-CLEA-lipase showed an Amide I and Amide V bands which originated from silanized magnetic nanoparticles (MNP) and a new band, Amide II was formed prior to the successful CLEA immobilization of lipase enzyme with MNPs 2020 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/79841/41/79841%20acceptance%20letter%20and%20report.pdf application/pdf en http://irep.iium.edu.my/79841/1/1.%20Amalin%20ICSET%202020%20Paper%20for%20Publication.pdf application/pdf en http://irep.iium.edu.my/79841/7/6.%20Amalin%20ICSET%202020%20Slide%20Presentation.pdf Ab Aziz Al Safi, Nur Amalin and Yusof, Faridah (2020) Screening of macromolecular cross-linkers and food-grade additives for enhancement of catalytic performance of MNP-CLEA-lipase of Hevea brasiliensis. In: The 1st International Conference of Science, Engineering and Technology (ICSET 2020), 27th February 2020, Pulau Pinang. (Unpublished) |
| spellingShingle | T Technology (General) TP155 Chemical engineering TP248.13 Biotechnology Ab Aziz Al Safi, Nur Amalin Yusof, Faridah Screening of macromolecular cross-linkers and food-grade additives for enhancement of catalytic performance of MNP-CLEA-lipase of Hevea brasiliensis |
| title | Screening of macromolecular cross-linkers and food-grade additives for enhancement of catalytic performance of MNP-CLEA-lipase of Hevea brasiliensis |
| title_full | Screening of macromolecular cross-linkers and food-grade additives for enhancement of catalytic performance of MNP-CLEA-lipase of Hevea brasiliensis |
| title_fullStr | Screening of macromolecular cross-linkers and food-grade additives for enhancement of catalytic performance of MNP-CLEA-lipase of Hevea brasiliensis |
| title_full_unstemmed | Screening of macromolecular cross-linkers and food-grade additives for enhancement of catalytic performance of MNP-CLEA-lipase of Hevea brasiliensis |
| title_short | Screening of macromolecular cross-linkers and food-grade additives for enhancement of catalytic performance of MNP-CLEA-lipase of Hevea brasiliensis |
| title_sort | screening of macromolecular cross-linkers and food-grade additives for enhancement of catalytic performance of mnp-clea-lipase of hevea brasiliensis |
| topic | T Technology (General) TP155 Chemical engineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/79841/ http://irep.iium.edu.my/79841/41/79841%20acceptance%20letter%20and%20report.pdf http://irep.iium.edu.my/79841/1/1.%20Amalin%20ICSET%202020%20Paper%20for%20Publication.pdf http://irep.iium.edu.my/79841/7/6.%20Amalin%20ICSET%202020%20Slide%20Presentation.pdf |