The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis
Background The genetic features indicate a crucial role in nephrolithiasis. The present study was aimed to investigate the role of Glutathione-S-transferase Mu (GSTM1), Glutathione-S- transferase Theta (GSTT1) and endothelial nitric oxide synthase (eNOs) gene polymorphism in nephrolithiasis. Met...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
2019
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| Online Access: | http://irep.iium.edu.my/79829/ http://irep.iium.edu.my/79829/1/79829_The%20endothelial%20nitric%20oxide_MYRA.pdf http://irep.iium.edu.my/79829/2/79829_The%20endothelial%20nitric%20oxide_SCOPUS.pdf http://irep.iium.edu.my/79829/3/79829_The%20endothelial%20nitric%20oxide_WOS.pdf |
| _version_ | 1848788853432778752 |
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| author | Mehdi, Wesen Adel Mehde, Atheer Awad Yusof, Faridah Ahmad Raus, Raha Resen, Ali Khudhair Ghazali, Hamid |
| author_facet | Mehdi, Wesen Adel Mehde, Atheer Awad Yusof, Faridah Ahmad Raus, Raha Resen, Ali Khudhair Ghazali, Hamid |
| author_sort | Mehdi, Wesen Adel |
| building | IIUM Repository |
| collection | Online Access |
| description | Background
The genetic features indicate a crucial role in nephrolithiasis. The present study was aimed to investigate the role of Glutathione-S-transferase Mu (GSTM1), Glutathione-S- transferase Theta (GSTT1) and endothelial nitric oxide synthase (eNOs) gene polymorphism in nephrolithiasis.
Methods
We involved a case-control study in which 480 individuals were divided into 240 healthy control and 240 patients with nephrolithiasis. For each patient and control, we measured biochemical criteria, levels of glutathione S-transferase, eNOs, GSTM1, GSTT1genes and eNOS genes polymorphism by PCR-RFLP.
Results
GSTM1 and GSTT1 null genotypes are not a risk features for nephrolithiasis. The eNOS frequency GG, GT, and TT genotypes by using Ban II enzyme as restriction enzyme were found to be (48.33, 36.67, and 15.00) %. The eNOS frequency TT, GT, and GG genotypes by using the Ban II enzyme as restriction enzyme were found to be 15.84, 25.83, and 58.33%, respectively. The result showed an increase in serum eNOs levels were in the patient's group comparing to control.
Conclusions
This work is the first in the literature to study the relation between eNOs genes polymorphisms and nephrolithiasis. The results conclude that TT genotypes in the eNOs genes are associated with an increase the oxidative stress in patients. |
| first_indexed | 2025-11-14T17:47:25Z |
| format | Article |
| id | iium-79829 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:47:25Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-798292020-03-25T07:13:24Z http://irep.iium.edu.my/79829/ The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis Mehdi, Wesen Adel Mehde, Atheer Awad Yusof, Faridah Ahmad Raus, Raha Resen, Ali Khudhair Ghazali, Hamid R Medicine (General) TP248.13 Biotechnology Background The genetic features indicate a crucial role in nephrolithiasis. The present study was aimed to investigate the role of Glutathione-S-transferase Mu (GSTM1), Glutathione-S- transferase Theta (GSTT1) and endothelial nitric oxide synthase (eNOs) gene polymorphism in nephrolithiasis. Methods We involved a case-control study in which 480 individuals were divided into 240 healthy control and 240 patients with nephrolithiasis. For each patient and control, we measured biochemical criteria, levels of glutathione S-transferase, eNOs, GSTM1, GSTT1genes and eNOS genes polymorphism by PCR-RFLP. Results GSTM1 and GSTT1 null genotypes are not a risk features for nephrolithiasis. The eNOS frequency GG, GT, and TT genotypes by using Ban II enzyme as restriction enzyme were found to be (48.33, 36.67, and 15.00) %. The eNOS frequency TT, GT, and GG genotypes by using the Ban II enzyme as restriction enzyme were found to be 15.84, 25.83, and 58.33%, respectively. The result showed an increase in serum eNOs levels were in the patient's group comparing to control. Conclusions This work is the first in the literature to study the relation between eNOs genes polymorphisms and nephrolithiasis. The results conclude that TT genotypes in the eNOs genes are associated with an increase the oxidative stress in patients. 2019-11-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/79829/1/79829_The%20endothelial%20nitric%20oxide_MYRA.pdf application/pdf en http://irep.iium.edu.my/79829/2/79829_The%20endothelial%20nitric%20oxide_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/79829/3/79829_The%20endothelial%20nitric%20oxide_WOS.pdf Mehdi, Wesen Adel and Mehde, Atheer Awad and Yusof, Faridah and Ahmad Raus, Raha and Resen, Ali Khudhair and Ghazali, Hamid (2019) The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis. International Journal of Biological Macromolecules, 140. pp. 719-726. ISSN 0141-8130 E-ISSN 1879-0003 https://www.sciencedirect.com/science/article/pii/S014181301934735X?via%3Dihub 10.1016/j.ijbiomac.2019.08.184 |
| spellingShingle | R Medicine (General) TP248.13 Biotechnology Mehdi, Wesen Adel Mehde, Atheer Awad Yusof, Faridah Ahmad Raus, Raha Resen, Ali Khudhair Ghazali, Hamid The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis |
| title | The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis |
| title_full | The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis |
| title_fullStr | The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis |
| title_full_unstemmed | The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis |
| title_short | The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis |
| title_sort | endothelial nitric oxide synthase gene g894t, glutathione s-transferase (gstm1 and gstt1) polymorphisms as a risk factor in the patient with nephrolithiasis |
| topic | R Medicine (General) TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/79829/ http://irep.iium.edu.my/79829/ http://irep.iium.edu.my/79829/ http://irep.iium.edu.my/79829/1/79829_The%20endothelial%20nitric%20oxide_MYRA.pdf http://irep.iium.edu.my/79829/2/79829_The%20endothelial%20nitric%20oxide_SCOPUS.pdf http://irep.iium.edu.my/79829/3/79829_The%20endothelial%20nitric%20oxide_WOS.pdf |