Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia
Previous study found that microRNA-101 (miR-101) and microRNA-744 (miR-744) were deregulated in head and neck cancers and were implicated in nasopharyngeal carcinoma (NPC) carcinogenesis. Thus, this study aimed to determine the expression of miR-101 and miR-744 in NPC and analyse the utility of t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Walailak University
2019
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| Online Access: | http://irep.iium.edu.my/79822/ http://irep.iium.edu.my/79822/1/79822_Differential%20Expression%20of%20miR-101%20and%20miR-744_article.pdf http://irep.iium.edu.my/79822/2/79822_Differential%20Expression%20of%20miR-101%20and%20miR-744_scopus.pdf |
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| author | Ahmad, Azmir Ab. Wahid, Haziq Leman, Wan Ishlah Othman, Noor Syamila Abd Rahman, Azlina Abdullah, Kahairi Muhammad, Siti Aeshah @ Naznin Kaderi, Mohd Arifin |
| author_facet | Ahmad, Azmir Ab. Wahid, Haziq Leman, Wan Ishlah Othman, Noor Syamila Abd Rahman, Azlina Abdullah, Kahairi Muhammad, Siti Aeshah @ Naznin Kaderi, Mohd Arifin |
| author_sort | Ahmad, Azmir |
| building | IIUM Repository |
| collection | Online Access |
| description | Previous study found that microRNA-101 (miR-101) and microRNA-744 (miR-744) were
deregulated in head and neck cancers and were implicated in nasopharyngeal carcinoma (NPC)
carcinogenesis. Thus, this study aimed to determine the expression of miR-101 and miR-744 in NPC and
analyse the utility of these microRNAs (miRNAs) as diagnostic biomarkers. Total RNA was extracted
from 31 NPC and 7 non-NPC control formalin-fixed paraffin-embedded (FFPE) samples. Complementary
DNA (cDNA) was synthesized from the total RNA and proceeded with quantitative real-time polymerase
chain reaction. Differential expression of miR-101 and miR-744 were calculated from quantification
cycle (Cq) data using 2-ΔΔCq calculation. The performance of these miRNAs were calculated using
receiver operating characteristic (ROC) curve analysis. The differential expression for miR-101 and miR744 were -1.39 (p < 0.05) and 2.48 (p > 0.05), respectively, where the deregulations were consistent with
the previous report. The area under curve for miR-101, miR-744 and combination of miR-101 and miR744 were 0.654 (95 % CI: 0.465 - 0.844), 0.588 (95 % CI: 0.368 - 0.808) and 0.626 (95 % CI: 0.481 -
0.771), respectively. However, re-analysis using balanced sample size between NPC and non-NPC
control group showed the value decreased to 0.653 (95 % CI: 0.347 - 0.959) for miR-101 but increased to
0.827 (95 % CI: 0.601 - 1.000) for miR-744 and 0.758 (95 % CI: 0.576 - 0.939) for the combination of
miR-101 and miR-744, indicating the importance of having a balanced sample size. We have successfully
determined the expression of miR-101 and miR-744 in NPC samples. We also demonstrated statistically
the utility of these miRNAs as diagnostic biomarkers. |
| first_indexed | 2025-11-14T17:47:24Z |
| format | Article |
| id | iium-79822 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:47:24Z |
| publishDate | 2019 |
| publisher | Walailak University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-798222022-06-03T07:48:01Z http://irep.iium.edu.my/79822/ Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia Ahmad, Azmir Ab. Wahid, Haziq Leman, Wan Ishlah Othman, Noor Syamila Abd Rahman, Azlina Abdullah, Kahairi Muhammad, Siti Aeshah @ Naznin Kaderi, Mohd Arifin R Medicine (General) RC Internal medicine Previous study found that microRNA-101 (miR-101) and microRNA-744 (miR-744) were deregulated in head and neck cancers and were implicated in nasopharyngeal carcinoma (NPC) carcinogenesis. Thus, this study aimed to determine the expression of miR-101 and miR-744 in NPC and analyse the utility of these microRNAs (miRNAs) as diagnostic biomarkers. Total RNA was extracted from 31 NPC and 7 non-NPC control formalin-fixed paraffin-embedded (FFPE) samples. Complementary DNA (cDNA) was synthesized from the total RNA and proceeded with quantitative real-time polymerase chain reaction. Differential expression of miR-101 and miR-744 were calculated from quantification cycle (Cq) data using 2-ΔΔCq calculation. The performance of these miRNAs were calculated using receiver operating characteristic (ROC) curve analysis. The differential expression for miR-101 and miR744 were -1.39 (p < 0.05) and 2.48 (p > 0.05), respectively, where the deregulations were consistent with the previous report. The area under curve for miR-101, miR-744 and combination of miR-101 and miR744 were 0.654 (95 % CI: 0.465 - 0.844), 0.588 (95 % CI: 0.368 - 0.808) and 0.626 (95 % CI: 0.481 - 0.771), respectively. However, re-analysis using balanced sample size between NPC and non-NPC control group showed the value decreased to 0.653 (95 % CI: 0.347 - 0.959) for miR-101 but increased to 0.827 (95 % CI: 0.601 - 1.000) for miR-744 and 0.758 (95 % CI: 0.576 - 0.939) for the combination of miR-101 and miR-744, indicating the importance of having a balanced sample size. We have successfully determined the expression of miR-101 and miR-744 in NPC samples. We also demonstrated statistically the utility of these miRNAs as diagnostic biomarkers. Walailak University 2019-05 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/79822/1/79822_Differential%20Expression%20of%20miR-101%20and%20miR-744_article.pdf application/pdf en http://irep.iium.edu.my/79822/2/79822_Differential%20Expression%20of%20miR-101%20and%20miR-744_scopus.pdf Ahmad, Azmir and Ab. Wahid, Haziq and Leman, Wan Ishlah and Othman, Noor Syamila and Abd Rahman, Azlina and Abdullah, Kahairi and Muhammad, Siti Aeshah @ Naznin and Kaderi, Mohd Arifin (2019) Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia. Walailak Journal of Science and Technology, 16 (5). pp. 295-306. ISSN 1686-3933 E-ISSN 2228-835X http://wjst.wu.ac.th/index.php/wjst/article/view/6285 |
| spellingShingle | R Medicine (General) RC Internal medicine Ahmad, Azmir Ab. Wahid, Haziq Leman, Wan Ishlah Othman, Noor Syamila Abd Rahman, Azlina Abdullah, Kahairi Muhammad, Siti Aeshah @ Naznin Kaderi, Mohd Arifin Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia |
| title | Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia |
| title_full | Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia |
| title_fullStr | Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia |
| title_full_unstemmed | Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia |
| title_short | Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia |
| title_sort | differential expression of mir-101 and mir-744 in nasopharyngeal carcinoma in pahang state of malaysia |
| topic | R Medicine (General) RC Internal medicine |
| url | http://irep.iium.edu.my/79822/ http://irep.iium.edu.my/79822/ http://irep.iium.edu.my/79822/1/79822_Differential%20Expression%20of%20miR-101%20and%20miR-744_article.pdf http://irep.iium.edu.my/79822/2/79822_Differential%20Expression%20of%20miR-101%20and%20miR-744_scopus.pdf |