Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities

The stem bark of Calophyllum depressinervosum and Calophyllum buxifolium were extracted and examined for their antioxidant activities, together with cytotoxicity towards human cancer cells. The methanol extract of C. depressinervosum exhibited good DPPH and NO scavenging effects. The strongest BCB...

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Main Authors: Zamakshshari, Nor Hisam, Ee, Gwendoline Cheng Lian, Ismail, Intan Safinar, Ibrahim, Zalikha, Mah, Siau Hui
Format: Article
Language:English
English
English
Published: Elsevier Ltd 2019
Subjects:
Online Access:http://irep.iium.edu.my/79802/
http://irep.iium.edu.my/79802/1/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_article.pdf
http://irep.iium.edu.my/79802/2/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_scopus.pdf
http://irep.iium.edu.my/79802/3/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_wos.pdf
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author Zamakshshari, Nor Hisam
Ee, Gwendoline Cheng Lian
Ismail, Intan Safinar
Ibrahim, Zalikha
Mah, Siau Hui
author_facet Zamakshshari, Nor Hisam
Ee, Gwendoline Cheng Lian
Ismail, Intan Safinar
Ibrahim, Zalikha
Mah, Siau Hui
author_sort Zamakshshari, Nor Hisam
building IIUM Repository
collection Online Access
description The stem bark of Calophyllum depressinervosum and Calophyllum buxifolium were extracted and examined for their antioxidant activities, together with cytotoxicity towards human cancer cells. The methanol extract of C. depressinervosum exhibited good DPPH and NO scavenging effects. The strongest BCB inhibition and FIC effects were shown by dichloromethane and ethyl acetate extracts of both species. Overall, DPPH, FRAP and FIC assays showed strong correlation with TPC. For cytotoxicity, hexane extract of C. depressinervosum possessed the strongest anti-proliferative activities towards SNU-1 cells while the hexane extract of C. buxifolium showed the strongest activity towards LS-174T and K562 cells with the IC values ranging from 7 to 17 μg/mL. The purification of plant extracts afforded eight xanthones, ananixanthone (1), caloxanthone B (2), caloxanthone I (3), caloxanthone J (4) xanthochymone B (5), thwaitesixanthone (6), 1,3,5,6-tetrahydroxyxanthone (7) and dombakinaxanthone (8). All the xanthones, except 1 were reported for the first time from both Calophyllum species. The xanthones were examined for their cytotoxic effect against K562 leukemic cells. Compounds 1 and 2 showed strong cytotoxicity with the IC values of 2.96 and 1.23 μg/mL, respectively. The molecular binding interaction of 2 was further investigated by performing molecular docking study with promising protein receptor Src kinase.
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spelling iium-798022020-06-24T09:03:15Z http://irep.iium.edu.my/79802/ Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities Zamakshshari, Nor Hisam Ee, Gwendoline Cheng Lian Ismail, Intan Safinar Ibrahim, Zalikha Mah, Siau Hui QR Microbiology RC Internal medicine SB Plant culture The stem bark of Calophyllum depressinervosum and Calophyllum buxifolium were extracted and examined for their antioxidant activities, together with cytotoxicity towards human cancer cells. The methanol extract of C. depressinervosum exhibited good DPPH and NO scavenging effects. The strongest BCB inhibition and FIC effects were shown by dichloromethane and ethyl acetate extracts of both species. Overall, DPPH, FRAP and FIC assays showed strong correlation with TPC. For cytotoxicity, hexane extract of C. depressinervosum possessed the strongest anti-proliferative activities towards SNU-1 cells while the hexane extract of C. buxifolium showed the strongest activity towards LS-174T and K562 cells with the IC values ranging from 7 to 17 μg/mL. The purification of plant extracts afforded eight xanthones, ananixanthone (1), caloxanthone B (2), caloxanthone I (3), caloxanthone J (4) xanthochymone B (5), thwaitesixanthone (6), 1,3,5,6-tetrahydroxyxanthone (7) and dombakinaxanthone (8). All the xanthones, except 1 were reported for the first time from both Calophyllum species. The xanthones were examined for their cytotoxic effect against K562 leukemic cells. Compounds 1 and 2 showed strong cytotoxicity with the IC values of 2.96 and 1.23 μg/mL, respectively. The molecular binding interaction of 2 was further investigated by performing molecular docking study with promising protein receptor Src kinase. Elsevier Ltd 2019-11 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/79802/1/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_article.pdf application/pdf en http://irep.iium.edu.my/79802/2/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_scopus.pdf application/pdf en http://irep.iium.edu.my/79802/3/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_wos.pdf Zamakshshari, Nor Hisam and Ee, Gwendoline Cheng Lian and Ismail, Intan Safinar and Ibrahim, Zalikha and Mah, Siau Hui (2019) Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities. Food and Chemical Toxicology, 133. ISSN 0278-6915 E-ISSN 873-6351 https://www.sciencedirect.com/science/article/pii/S0278691519305903 10.1016/j.fct.2019.110800
spellingShingle QR Microbiology
RC Internal medicine
SB Plant culture
Zamakshshari, Nor Hisam
Ee, Gwendoline Cheng Lian
Ismail, Intan Safinar
Ibrahim, Zalikha
Mah, Siau Hui
Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities
title Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities
title_full Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities
title_fullStr Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities
title_full_unstemmed Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities
title_short Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities
title_sort cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities
topic QR Microbiology
RC Internal medicine
SB Plant culture
url http://irep.iium.edu.my/79802/
http://irep.iium.edu.my/79802/
http://irep.iium.edu.my/79802/
http://irep.iium.edu.my/79802/1/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_article.pdf
http://irep.iium.edu.my/79802/2/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_scopus.pdf
http://irep.iium.edu.my/79802/3/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_wos.pdf